FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067754
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT107960.5221123982833549No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT90940.4398008660604694No Hit
GTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAACT76570.3703051716983742No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC69850.3378061413495029No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC68650.3320027430729187No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC63850.3087891499665821No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGTATAATAACTGG60500.2925879964444513No Hit
CTGGTACCCCTGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACT58770.28422143059570915No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATT55630.26903587177198063No Hit
GTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTA55410.2679719154212735No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG55030.26613417263368855No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT53320.2578643300895561No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC53020.25641348052041No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT51230.2477567447578387No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT50820.2457739170133391No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC49030.23711718125076775No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCAGGGGTACCAGTGGCCCTGCT46930.22696123426674547No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA46590.22531693808837994No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT45980.222366877297783No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG43830.21196912205223636No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG43820.21192076039993152No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAG43130.20858380639089563No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG42360.2048599591634208No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT41760.20195826002512873No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41460.2005074104559827No Hit
GTCAGAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGTTG40560.19615486174854457No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT39130.18923914546894843No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA39080.1889973372074241No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC38890.1880784658136316No Hit
GAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGTTGCATC38080.1841611719769373No Hit
GGGTACCAGTGGCCCTGCTGGATGCAACAAAAATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG38080.1841611719769373No Hit
GAGTGAACTCTGTCCCAGACCCACTGCCACTGAACCTGGCAGGGGTACCA37920.1833873855400594No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC36110.1746339264728783No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC35500.17168386568228136No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGAGTTCACTCTCACCATCAGCAGCC35470.17153878072536674No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34320.16597719071030692No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT33620.1625918750489662No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATTTTTGTTGCATCCAGCAGGGCCACTGGTACCCCTGCCAGGTT33290.16099594052290553No Hit
CTCCCAGGCTCCTCATTTTTGTTGCATCCAGCAGGGCCACTGGTACCCCT30860.14924405901282262No Hit
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCCGCTCAGCTCCATGTAGG30610.14803501770520092No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT30300.14653580648375No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29940.14479478700077475No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29930.14474642534846988No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT29110.14078076985947072No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT28870.13962009020415386No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28870.13962009020415386No Hit
CTGCTACTCTGGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTC28320.13696019932738615No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28050.1356544347151547No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA28020.1355093497582401No Hit
GTTTATTTCTGTCAGCAGTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA27760.13425194679831354No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26930.13023792965700948No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC26750.12936741991552186No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA26680.1290288883493878No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA26660.12893216504477806No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCGGAGGCCAGTT26290.12714278390949793No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT26120.1263206358203152No Hit
CCCTGTCTGTGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGC25410.12288695850666954No Hit
CCTTGGTCCCTTGGCCGAACGTCGGAGGCCAGTTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGC25330.12250006528823061No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC25300.12235498033131602No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25150.12162955554674298No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24970.12075904580525537No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC24850.12017870597759694No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTTGCTGGTA24780.11984017441146286No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA24750.11969508945454826No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG24360.11780898501465842No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC24110.11659994370703672No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA24040.11626141214090265No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC23780.11500400918097607No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT23670.11447203100562252No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23630.11427858439640305No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTCTC23250.11244084160881808No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGGTAACACTCTGACTGGCCCT23230.11234411830420835No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC23130.11186050178115965No Hit
GTCTATTACTGTGCGAGATGTGATACAGCTGTGGCCACGCTCTTTGACTA22790.11021620560279415No Hit
GAAATAAACTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTCCCAGACCCACTGCCA22720.10987767403666006No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC22580.1092006109043919No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22470.10866863272903837No Hit
GTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCG21790.10538004037230733No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC21760.10523495541539274No Hit
GGCTCCCAGAGACCAGTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCGGGGGAAAGAG21680.1048480621969538No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT21250.10276851114784447No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA21200.10252670288632014No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTA21000.10155946984022277No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG20950.10131766157869843No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC20920.10117257662178383No Hit
TTATTATACTGCTGACAGAAATAAACTGCAAAATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAACTCTGTC20920.10117257662178383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)