FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668636
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22360.33441214651918233No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAAAACATGGATGCCAGTGCCCCAGGTCTGA19030.2846092642334544No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16880.25245425014507145No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAAAACATGGATGCCAGTGCCCCAGGTCTGACAGTAATAGT15690.23465682374266417No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15490.23166565964141927No Hit
GTAATATACAGCCGTGTCTGCGGCGGTGACAGAGTTCAGCTTCAGGGAGA15290.22867449554017433No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG13770.2059416483707129No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13510.20205313503909453No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT13320.19921152914291182No Hit
GTATATTACTGTGCGAGACAAGCAGCTAGGGATAGTAGTGGTTATTACTACGTTCCTTTTGGCGGTATTGACTT13210.19756638888722713No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC12560.18784510555818112No Hit
ACGTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGC12430.18590084889237193No Hit
CTGTTAACGTTGATCAAGAATCGAGGGCCCTTCTCTGGCTGCTGTTGATGCCATGCGATGGCGTAGTTGCTGTGC12030.17991852068988207No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12010.1796194042797576No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAACTACGCCATCGCATGGCATCAA12000.17946984607469535No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAACTACGCCATCGCATGGCATC11730.1754317745380147No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11410.17064591197602283No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAAATGGTGAGGTAGC10940.16361667633809726No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG10880.16271932710772377No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10790.16137330326216356No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATCCATGTTTTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGT10690.15987772121154112No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10620.1588308137761054No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG10470.1565874407001717No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT10040.1501564378824951No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9810.14671659916606344No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT9790.14641748275593897No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9730.14552013352556548No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9650.1443236678850675No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT9600.14357587685975629No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG9550.14282808583444506No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9470.14163162019394707No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG9370.14013603814332462No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9240.1381917814775154No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9230.1380422232724532No Hit
GGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCACGCTCAC9110.1362475248117062No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC8580.12832093994340718No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC8290.12398375199660205No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC8040.12024479687004587No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATCCATGTTTTATTCGGCGGAG7980.11934744763967241No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAAATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC7950.11889877302448566No Hit
GCACAGGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCACG7820.11695451635867647No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7570.11321556123212032No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATTTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC7530.11261732841187135No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7510.11231821200174683No Hit
ACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAAAACATGGATGCCAG7130.10663500020938149No Hit
TCCTCAGACTGGAGGCTGGAAATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATC7120.10648544200431924No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7060.10558809277394576No Hit
CCACTGCACAGGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT6950.10394295251826106No Hit
CAGCAAGGGGGACGGGATCCCTGATCGCTTCTCAGGCTCCAGCTCTGGGGCTGAGCGCTACCTCACCATTTCCAG6930.10364383610813657No Hit
GGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGT6810.10184913764738962No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC6810.10184913764738962No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA6770.10125090482714062No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT6740.10080223021195389No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT4250.071.1591970
TACGTAT400.00890263549.3086770
CTACGGA4150.047.7511469
GGTATCA7600.047.4161451
CGTATGC803.274181E-1143.2199841
TCTCGTA651.1734301E-842.31035638
TCGTATG901.2187229E-1038.3389940
TCTACGG4650.038.19812868
GACGTGT4400.037.69668262
TCGTAGT700.001707391537.56851670
GTATCAA18400.036.95271
TTGGACG4500.036.5055459
ACGTGTC4600.036.14374563
GTCTACG4750.035.99891367
ATCTCCG1052.0008883E-1135.5993810
ATCTCGT807.4003765E-834.3304137
TGGACGT4850.033.99402260
CGAACTA400.002793665533.9863124
AAGGCGC502.0329119E-433.9837723
AGGGGGG1756.2918843E-933.81166570
Produced by FastQC (version 0.11.5)