FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences668636
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20420.30539785473710657No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAAAACATGGATGCCAGTGCCCCAGGTCTGA17540.2623250916791797No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17170.2567914380918766No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15310.22897361195029883No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14400.21536381528963444No Hit
GACCTAGGACGGTCAGCGTGGTCCCTCCGCCGAATAAAACATGGATGCCAGTGCCCCAGGTCTGACAGTAATAGT13800.20639032298589965No Hit
GTAATATACAGCCGTGTCTGCGGCGGTGACAGAGTTCAGCTTCAGGGAGA13750.2056425319605884No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC13630.20384783349984148No Hit
GTATATTACTGTGCGAGACAAGCAGCTAGGGATAGTAGTGGTTATTACTACGTTCCTTTTGGCGGTATTGACTT12590.18829378017336787No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12530.1873964309429944No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT12180.1821618937658158No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG12050.1802176371000066No Hit
CACCTGCACTCTGAGCAGTGGGCACAGCAACTACGCCATCGCATGGCATC11800.17647868197345043No Hit
ACGTTAACAGTGATGGCAGCCACAGCAAGGGGGACGGGATCCCTGATCGC11760.17588044915320145No Hit
CTGTTAACGTTGATCAAGAATCGAGGGCCCTTCTCTGGCTGCTGTTGATGCCATGCGATGGCGTAGTTGCTGTGC11570.17303884325701877No Hit
GGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCACGCTCAC11560.17288928505195653No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG11530.17244061043676978No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11330.16944944633552486No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCTC11280.16870165531021364No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG11090.16586004941403096No Hit
CCTGCACTCTGAGCAGTGGGCACAGCAACTACGCCATCGCATGGCATCAA10940.16361667633809726No Hit
GACTATTACTGTCAGACCTGGGGCACTGGCATCCATGTTTTATTCGGCGGAGGGACCACGCTGACCGTCCTAGGT10480.15673699890523396No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10200.15254936916349104No Hit
GCTCTGGGGAGTCTGCACCATGGCTTGGACCCCACTCCTCTTCCTCACCCTCCTCCTCCACTGCACAGGGTCTCT10190.1523998109584288No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA9680.14477234250025425No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAAATGGTGAGGTAGC9450.1413325037838226No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA9410.1407342709635736No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG9290.13893957250282665No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT9250.13834133968257767No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC9190.1374439904522042No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC9020.134901500966146No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG8900.13310680250539905No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT8770.13116254583958986No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG8680.12981652199402965No Hit
GCCCCAGAGCTGGAGCCTGAGAAGCGATCAGGGATCCCGTCCCCCTTGCT8660.12951740558390515No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8440.12622712507253572No Hit
GCACAGGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGTCACG7960.11904833122954792No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC7820.11695451635867647No Hit
CCTCCTCCACTGCACAGGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGC7760.116057167128303No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7680.11486070148780503No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7550.11291644482199584No Hit
CCACTGCACAGGGTCTCTCTCCCAACTTGTACTGACTCAATCGCCCTCTGCCTCTGCCTCCCTGGGAGCCTCGGT7540.11276688661693357No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC7490.11201909559162235No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA7450.11142086277137336No Hit
CTATTACTGTCAGACCTGGGGCACTGGCATCCATGTTTTATTCGGCGGAG7430.11112174636124887No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7360.11007483892581316No Hit
GCTCTGGGGCTGAGCGCTACCTCACCATTTCCAGCCTCCAGTCTGAGGATGAGGCTGACTATTACTGTCAGAC7360.11007483892581316No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT7330.10962616431062641No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGGCTGGAAATGGTGAGGTAGCGCTCAGCCCCAGAGCTGGAGC7270.10872881508025294No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6720.1005031138018294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)