FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958091
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT39930.4167662570674393No Hit
GTATATTACTGTGCAACAGGAAGTTTTGTAGTACTGCCAACTTCTGTACCTTTTGACTCCTGGGGCCAGGGAACC30270.315940761368179No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28360.2960052855104578No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA27530.2873422253209768No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25900.27032922759946604No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA21940.22899703681591835No Hit
GTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGTCTGGTACC21170.2209602219413396No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18990.19820664216655828No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18920.19747602263250566No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17680.18453361945785943No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16890.17628805614497997No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16690.17420057176197248No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATT16330.17044309987255907No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15680.16365877562778483No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC15260.15927505842346917No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15230.15896193576601805No Hit
GTCTCCAGGCATGTTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTGTCTTGCAGGGCCAGTCAGAGTGTTAGCAG14870.1552044638766046No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14820.15468259278085275No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14760.15405634746595054No Hit
TTATTACTGTCAGCAGTATGGTGGCTCACCTCGATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14680.15322135371274753No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG14380.15009012713823633No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14090.14706327478287554No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAAATTGCTGCTGGTACCAGACTAAGTAGCTGCTGCTAACACTCTGA14040.14654140368712368No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT13860.14466266774241696No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13690.14288830601686062No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13630.14226206070195838No Hit
GTATGGTGGCTCACCTCGATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13240.13819146615509384No Hit
CTCCAGGCATGTTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTGTCTTGC13060.13631273021038712No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13050.13620835599123673No Hit
GTACAGAAGTTGGCAGTACTACAAAACTTCCTGTTGCACAGTAATATACGGCCGTGTCCTCAGATCTCAGGCTG12830.13391212316992854No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT12790.13349462629332703No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12780.13339025207417665No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12700.13255525832097367No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12670.13224213566352258No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12650.13203338722522184No Hit
CAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12600.13151151612946996No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG12380.12921528330816176No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12370.12911090908901138No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12360.12900653486986102No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12340.12879778643156026No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12230.12764967002090616No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12220.1275452958017558No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11970.12493594032299646No Hit
CATCTACAGACACAGCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGAC11740.12253533328253788No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11640.12149159109103415No Hit
GAGTTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTACTGTGCAAC11120.11606413169521475No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG11090.11575100903776364No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC11030.1151247637228614No Hit
GGCCAAAAGTGTATCGAGGTGAGCCACCATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTG10920.11397664731220729No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10760.11230665980580133No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10690.11157604027174871No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10670.11136729183344797No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTACTGTGCAACAGGAAGTTTTGTAGTA10610.11074104651854573No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGTCTGGTACCAGCAGCAATTTGGC10590.11053229808024498No Hit
CTTCTGTACCTTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCC10560.11021917542279387No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT10500.10959293010789165No Hit
GTCTGGTACCAGCAGCAATTTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATC10470.10927980745044051No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTATCGAGGTGAGCCACCATACTG10470.10927980745044051No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT10270.10719232306743305No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10180.10625295509507969No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10090.10531358712272634No Hit
CTGCTACTCTGGCTCCCAGAGACCACCGGAGAAATTGTGTTGACGCAGTC10040.10479171602697447No Hit
GTGCAGGTGAAGGTCTCCCCATGGTTCCATGGCTGGGCACAGCCAGGCAGGACACTGGACACGCTGTAGCAGCCA9940.10374797383547074No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC9880.10312172852056851No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC9720.10145174101416254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)