FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences958091
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTAGGCTGTGTCTGTAGATGTGTCCTCGGT39830.4157225148759356No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA28920.30185024178287867No Hit
GTATATTACTGTGCAACAGGAAGTTTTGTAGTACTGCCAACTTCTGTACCTTTTGACTCCTGGGGCCAGGGAACC28390.2963184081679089No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT27930.2915171940869917No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21510.2245089453924523No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA21220.22148209303709146No Hit
GTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCAGCTACTTAGTCTGGTACC19790.20655657969858815No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19250.200920371864468No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC17140.1788974116237393No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16870.17607930770667923No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16720.17451369441942363No Hit
GTCTGGGACAGACTTCACTCTCACTATCAGCAGACTGGAGCCTGAAGATT16470.1719043389406643No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16150.16856436392785237No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15510.1618844139022285No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15380.16052754905327363No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15340.16011005217667215No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15320.15990130373837141No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14480.15113386932974007No Hit
GTCTCCAGGCATGTTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTGTCTTGCAGGGCCAGTCAGAGTGTTAGCAG14020.1463326552488229No Hit
GTATGGTGGCTCACCTCGATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT13470.14059207319555242No Hit
GTACAGAAGTTGGCAGTACTACAAAACTTCCTGTTGCACAGTAATATACGGCCGTGTCCTCAGATCTCAGGCTG13380.13965270522319906No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG13190.13766959505934195No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13110.13683460130613898No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12970.13537336223803376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12970.13537336223803376No Hit
TTATTACTGTCAGCAGTATGGTGGCTCACCTCGATACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA12880.1344339942656804No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGGT12730.13286838097842482No Hit
CTCCAGGCATGTTGTCTTTGTCTCCAGGGGAGAGAGCCACCCTGTCTTGC12580.13130276769116922No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA12510.1305721481571166No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC12450.12994590284221436No Hit
CCATAGATGAGGAGCCTGGGAGGCTGGCCAAATTGCTGCTGGTACCAGACTAAGTAGCTGCTGCTAACACTCTGA12420.12963278018476324No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12150.12681467626770318No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG11930.12451844344639497No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA11900.12420532078894385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11800.12316157859744012No Hit
CAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTGCTGATAGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11780.12295283015913937No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11760.12274408172083863No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11440.1194041067080267No Hit
CATCTACAGACACAGCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGAC11410.11909098405057557No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG11410.11909098405057557No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11380.11877786139312446No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11370.11867348717397408No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11270.11762974498247035No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11260.11752537076331997No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11220.11710787388671848No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGTATCGAGGTGAGCCACCATACTG10990.11470726684625991No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC10890.11366352465475618No Hit
GAGTTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTACTGTGCAAC10860.11335040199730506No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10740.11209791136750058No Hit
GTCTGGTACCAGCAGCAATTTGGCCAGCCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGCATC10730.1119935371483502No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10690.11157604027174871No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10690.11157604027174871No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAG10630.11094979495684648No Hit
GTCAGAGTGTTAGCAGCAGCTACTTAGTCTGGTACCAGCAGCAATTTGGC10550.11011480120364349No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10450.10907105901213976No Hit
GGCCAAAAGTGTATCGAGGTGAGCCACCATACTGCTGACAGTAATAAAGTGCAAAATCTTCAGGCTCCAGTCTG10320.10771419416318491No Hit
CTTCTGTACCTTTTGACTCCTGGGGCCAGGGAACCCTGGTCACCGTCTCC10150.10593983243762858No Hit
GGGTTCCCTGGCCCCAGGAGTCAAAAGGTACAGAAGTTGGCAGTACTACAAAACTTCCTGTTGCACAGTAATATA10020.10458296758867373No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10010.10447859336952334No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9960.10395672227377149No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG9890.10322610273971887No Hit
GCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGGTGCTGT9890.10322610273971887No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT9820.10249548320566627No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGACACGGCCGTATATTACTGTGCAACAGGAAGTTTTGTAGTA9600.10019925038435805No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9590.1000948761652077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)