FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672093
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49570.2964548024541697No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC48040.2873045937038191No Hit
CTATTATTGTCACCAATATGCAACCCCACCGTGGACGTTCGGCCAGGGGA45870.27432684665266827No Hit
GTCTTTGTCTGCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGC44750.2676286546262678No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGTA37200.2224756637340148No Hit
CAATAATAGACTGCAGAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGATTCACTGGCA36670.2193059835786646No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35170.210335190686164No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG30780.18408067015411225No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30270.18103060057066203No Hit
GTCTCCAGGCGCCCTGTCTTTGTCTGCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTTTTACCGG30080.17989430013761196No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29710.17768150455746182No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA29450.1761265671227617No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29040.17367455039881155No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27470.16428512050466093No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27270.16308901478566085No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26510.15854381305346055No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26480.15836439719561052No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26010.15555354875596034No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25430.15208484217086013No Hit
GTGATATACGGCCGTGTCCTCGGCTCTCAGGAAGTTCATGTGAAGAAAAA25160.15047009945021No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24700.14771905629650983No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC24130.1443101549973596No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23900.1429346334205095No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23830.14251599641885948No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23710.14179833298745942No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC23250.13904728983375925No Hit
CTCCTGTGCAGCCTCTGAGTTCATCGTCAGTTACAACTACATGAGTTGGGTCCGCCAGCCTCCAGGGAAGGGA23190.13868845811805922No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23090.13809040525855917No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22730.13593741496435904No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC22460.13432267224370895No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT22260.13312656652470886No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21950.13127260266025872No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21910.1310333815164587No Hit
GTATATCACTGCGTCTACGATGTTTGGACTGGCCCAAGCCTCTGGGGCCA21640.12941863879580862No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21630.1293588335098586No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT21390.1279235066470585No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT21330.12756467493135848No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTCTGCAGTCTATTATTGTCACCAATATGCAACCCCA21270.12720584321565845No Hit
GTCTATTATTGTCACCAATATGCAACCCCACCGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAA21230.12696662207185844No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21170.12660779035615843No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21130.1263685692123584No Hit
CTCCTGCAGGGCCAGTCAGCGTTTTACCGGCAGCCACTTAGCCTGGTTGC21020.12571071106690837No Hit
CTCCCAGACTCCTCATCTTTGGTGGTTACACCAGGGCCCCTGGCATCCCA20940.12523226877930832No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20880.1248734370636083No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20430.12218219919585814No Hit
ATAATAGACTGCAGAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGATTCACTGGCA19950.11931154547025795No Hit
ACACAAACCTGGCCAGGCTCCCAGACTCCTCATCTTTGGTGGTTACACCAGGGCCCCTGGCATCCCAGACAGGTT19910.11907232432645791No Hit
GAGTGAAGTCTGTCCCAGATTCACTGGCACTGAACCTGTCTGGGATGCCA19530.11679972346035777No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19370.1158428388851577No Hit
ATGTTTGGACTGGCCCAAGCCTCTGGGGCCAGGGAACCCTGCTCACCGTC19300.11542420188350767No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18850.1127329640157575No Hit
GTCCAAACATCGTAGACGCAGTGATATACGGCCGTGTCCTCGGCTCTCAG18550.11093880543725737No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18350.10974269971825729No Hit
GCCGTATATCACTGCGTCTACGATGTTTGGACTGGCCCAAGCCTCTGGGGCCAGGGAACCCTGCTCACCGTCTC18230.10902503628685725No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC18120.10836717814140721No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18040.10788873585380718No Hit
CACTTAGCCTGGTTGCAACACAAACCTGGCCAGGCTCCCAGACTCCTCATCTTTGGTGGTTACACCAGGGCCC18030.10782893056785717No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17960.10741029356620714No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17860.1068122407067071No Hit
CATTTATGTATTCCAATGATCACGCCGACTACGCAGACTCCGTGAGGGGCCGATTCACCGTCTCCAGAGACAATT17620.105376913843907No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17460.10442002926870694No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17370.10388178169515691No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17360.1038219764092069No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC17100.10226703897450681No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16880.10095132268360671No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16820.10059249096790669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)