FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672093
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49030.2932253170128695No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47400.2834770554030188No Hit
GTCTTTGTCTGCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGC44380.26541585904611764No Hit
CTATTATTGTCACCAATATGCAACCCCACCGTGGACGTTCGGCCAGGGGA43010.2572225348709671No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40580.24268985038511615No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37010.2213393633009647No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAGTA35260.21087343825971402No Hit
CAATAATAGACTGCAGAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGATTCACTGGCA33510.2004075132184633No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC29610.17708345169796177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29310.17528929311946165No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29060.17379416097071154No Hit
GTCTCCAGGCGCCCTGTCTTTGTCTGCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGCGTTTTACCGG27630.16524200507986098No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27570.16488317336416097No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27110.16213213021046077No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27050.16177329849476077No Hit
GTGATATACGGCCGTGTCCTCGGCTCTCAGGAAGTTCATGTGAAGAAAAA26620.15920167119891057No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26270.15710848619066045No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26240.15692907033281045No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25920.15501530118241033No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24430.14610431357585973No Hit
CTCCTGTGCAGCCTCTGAGTTCATCGTCAGTTACAACTACATGAGTTGGGTCCGCCAGCCTCCAGGGAAGGGA23900.1429346334205095No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23550.14084144841225935No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23530.14072183784035935No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA22970.13737274182715914No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22730.13593741496435904No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22720.13587760967840903No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22350.1336648140982589No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTTGGCTGAGCTGGGTTTTC21980.13145201851810875No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21610.1292392229379586No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTCTGCAGTCTATTATTGTCACCAATATGCAACCCCA21220.12690681678590846No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21200.12678720621400844No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC21180.12666759564210842No Hit
GTCTATTATTGTCACCAATATGCAACCCCACCGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAA21060.12594993221070838No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20790.12433518949005826No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20790.12433518949005826No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20590.12313908377105819No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC20330.12158414633635808No Hit
CTCCCAGACTCCTCATCTTTGGTGGTTACACCAGGGCCCCTGGCATCCCA19810.11847427146695788No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19530.11679972346035777No Hit
ACACAAACCTGGCCAGGCTCCCAGACTCCTCATCTTTGGTGGTTACACCAGGGCCCCTGGCATCCCAGACAGGTT19400.11602225474300772No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19370.1158428388851577No Hit
GAGTGAAGTCTGTCCCAGATTCACTGGCACTGAACCTGTCTGGGATGCCA19110.1142879014504576No Hit
GTATATCACTGCGTCTACGATGTTTGGACTGGCCCAAGCCTCTGGGGCCA19020.11374965387690755No Hit
ATGTTTGGACTGGCCCAAGCCTCTGGGGCCAGGGAACCCTGCTCACCGTC18910.11309179573145753No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18740.11207510587030745No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18730.11201530058435746No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18690.11177607944055742No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18310.10950347857445729No Hit
ATAATAGACTGCAGAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGAAGTCTGTCCCAGATTCACTGGCA18290.10938386800255727No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18240.10908484157280726No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18220.10896523100090724No Hit
CTCCTGCAGGGCCAGTCAGCGTTTTACCGGCAGCCACTTAGCCTGGTTGC17970.10747009885215715No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17820.10657301956290709No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC17800.10645340899100707No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTC17730.10603477198935704No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17700.10585535613150704No Hit
GCCGTATATCACTGCGTCTACGATGTTTGGACTGGCCCAAGCCTCTGGGGCCAGGGAACCCTGCTCACCGTCTC17680.10573574555960703No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17480.10453963984060695No Hit
GTCCAAACATCGTAGACGCAGTGATATACGGCCGTGTCCTCGGCTCTCAG17410.10412100283895692No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT17010.10172879140095677No Hit
GCCCAGCACTGGGATTCCGAGGTGTTTCCATTCGGTGATCAGCACTGAAC16940.10131015439930674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)