FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961506
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCGACAATAAGAACTACTTAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGCTACT53350.5548587320307934No Hit
ATATTATACTAGTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT44580.4636476527447567No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36760.38231690701878096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35290.36702839087847605No Hit
CAATAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATA25990.2703051255010369No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25810.2684330623001832No Hit
GTATAATATTGCTGACAGTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCC25250.2626088656753052No Hit
GTTTATTACTGTCAGCAATATTATACTAGTTGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA25080.26084080598561005No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA23050.23972809322042712No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22590.23494393170713446No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22080.2296397526380491No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21920.22797569645951246No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21360.22215149983463445No Hit
GTATTATTGTGCGAGAGTTCTAGGTAATGGCTGGTACGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTC21000.2184073734329271No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG20360.21175114871878073No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20120.2092550644509759No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19390.2016628081364027No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19100.19864670631280515No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18600.19344653075487828No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18430.19167847106518315No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17130.17815801461457337No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16920.1759739408802441No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16420.17077376532231728No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16030.16671762838713436No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15130.15735731238286604No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15090.1569412983382319No Hit
AACCAGGACAGCCTCCTAAGCTACTCTTTTACTGGGCATCTACCCGGGAA14920.15517323864853677No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC14510.15090909469103678No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14080.1464369437112197No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC14030.145916926155427No Hit
CTCCTAAGCTACTCTTTTACTGGGCATCTACCCGGGAATCCGGGGTCCCC13950.1450848980661587No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATA13800.14352484539878066No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT13780.14331683837646358No Hit
GTGTATTCCTGGCGTTGTCTCTGGAGATGGTGAATCGGCCCCTCACAGAGTCCACATAGAATTTGTCACTTCCAT13760.1431088313541465No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13650.1419647927314026No Hit
GTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGCCCCAGACCCGCTGCCA13140.13666061366231724No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13140.13666061366231724No Hit
CTATTGGATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGGCTGGAGCGGG13070.13593258908420747No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12930.13447653992798797No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC12900.13416452939451234No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12720.1322924661936587No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12420.1291723608589026No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCGA12370.12865234330310993No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC12180.1266762765910977No Hit
GTCCCCGACCGATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCAC11960.1243881993456099No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11860.12334816423402455No Hit
GTAAAAGAGTAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA11640.12106008698853674No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11630.1209560834773782No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11370.11825199218725624No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11360.1181479886760977No Hit
ATTCAGTGGCAGCGGGTCTGGGGCAGATTTCACTCTCACCATCAGCAGCC11090.11533989387481722No Hit
ATAATACAGAGCCGTGTCCTCGGCTCTCAGGCTGTTGATTTGCAGATACA11010.11450786578554892No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC10870.11305181662932941No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10820.11253179907353672No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10490.109099683205305No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT10380.1079556445825611No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10340.10753963053792695No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10320.1073316235156099No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10270.10681160595981722No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT10190.1059795778705489No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10130.10535555680359768No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT9950.10348349360274403No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGCGGCAGTTTATTACTGTCAGCAATATTATACTAGTTGGTGGACGTT9900.10296347604695136No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTATTGTCGGAGCTGTATAAAACACT9880.10275546902463427No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9830.10223545146884158No Hit
CCATAAAGGAAGATGGAAGTGACAAATTCTATGTGGACTCTGTGAGGGGCCGATTCACCATCTCCAGAGACAACG9810.1020274444465245No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG9790.10181943742420745No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9780.10171543391304891No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC9700.10088340582378062No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT9690.10077940231262209No Hit
GGAATACACTGTATCTGCAAATCAACAGCCTGAGAGCCGAGGACACGGCT9680.10067539880146353No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9670.100571395290305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)