FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922445
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC47480.5147190347391986No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT31870.3454948533516904No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCGCGAAATTGTTGCTGCCTGCATATGAG31770.3444107778783559No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCGCGAAATTGTTG28110.3047336155543149No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCACCATCTCCTG27850.30191501932364534No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26730.28977337402229947No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25750.2791494343836218No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25050.2715609060702806No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23670.25660066453826513No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC22940.24868691358292364No Hit
GAATATTACTGCAGCTCATATGCAGGCAGCAACAATTTCGCGGTATTCGG21350.2314501135569058No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20800.22548769845356634No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT19990.21670668711955726No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19390.21020223427955054No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19290.20911815880621606No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18990.20586593238621273No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18650.2021800757768756No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG18320.19860262671487192No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18150.19675969841020333No Hit
CCCTGAGTGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGAATATTACTGC17490.18960480028619592No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17430.18895435500219526No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17070.18505168329819122No Hit
CTTCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT17020.184509645561524No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCA16630.18028175121551962No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCAC16580.1797397134788524No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16320.17692111724818282No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16240.17605385686951525No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16190.17551181913284802No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15780.17106710969217676No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC15460.1675980681775065No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC15320.1660803625148383No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14820.160659985148166No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCGCGAAATTGTTGC14320.15523960778149373No Hit
GTAATATTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACACTCAGGGAGG13690.1484099322994867No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13530.14667541154215158No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13040.14136344172281273No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12800.13876166058681005No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12200.13225720774680333No Hit
GTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGA11750.1273788681167983No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG11630.12607797754879696No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11490.12456027188612871No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCAGGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA11460.12423504924412838No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11410.12369301150746115No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG11370.12325938131812737No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11310.12260893603412669No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT11290.1223921209394598No Hit
CACTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGAGCAGGGACCCCTGAGGGCCGCTTATTGACCT11250.12195849075012602No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT11210.12152486056079224No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11170.12109123037145847No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11100.12033237754012435No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11020.11946511716145677No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10980.119031486972123No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGCTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGTGT10680.11577926055211965No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCAC10500.11382792470011763No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10500.11382792470011763No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC10470.11350270205811729No Hit
GTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC10370.11241862658478283No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCA10060.10905799261744602No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAAC9990.1082991397861119No Hit
GGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTCAATAAG9870.10699824921811057No Hit
CCTCAGGGGTCCCTGCTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGTGTCTCTGGGCTCCAGG9760.10580576619744267No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA9670.10483009827144166No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9580.10385443034544066No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCACCATCT9570.1037460227981072No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG9450.10244513223010587No Hit
GGATGAGGCTGAATATTACTGCAGCTCATATGCAGGCAGCAACAATTTCG9380.10168627939877174No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9300.10081901902010418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTC303.7403952E-451.14666768
TACGGAC200.007791345551.0073213
GGTATCA7800.046.2217861
CGTATGC1300.042.5746641
TCGTATG1350.040.9210940
GTATCAA20950.038.1521721
GCCGTCT1500.037.15006646
GTATGCC1500.036.9530742
GTAGTTT1109.5789965E-636.2357270
TATACAC1700.036.0051653
TATCTCG1350.035.5826436
ACGGACT1500.034.0436427
GACGGAC1500.034.03255526
ATGCCGT1650.033.6774144
ATCTCGT1450.033.15950437
GAAATCG450.003569107732.3300165
CTAATTG450.003983274631.61319257
ATCAACG25450.031.5330123
TCAACGC25700.031.49094
CAACGCA25750.031.4297525
Produced by FastQC (version 0.11.5)