FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922445
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44300.4802454346871629No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCGCGAAATTGTTGCTGCCTGCATATGAG31950.3463621137303579No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT29630.3212115627489986No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCACCATCTCCTG27780.3011561664923112No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27330.29627782686230614No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25330.2745963173956171No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23930.25941926076893473No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCGCGAAATTGTTG23250.2520475475502605No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22880.24803646829892295No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22820.2473860230149223No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC20810.22559610600089977No Hit
GAATATTACTGCAGCTCATATGCAGGCAGCAACAATTTCGCGGTATTCGG20210.21909165316089307No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19370.20998541918488367No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19350.20976860409021675No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19120.20727523050154753No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCAC18450.20001192483020666No Hit
CCCTGAGTGTCTCTGGGCTCCAGGCTGAGGATGAGGCTGAATATTACTGC18060.1957840304842023No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG17440.1890627625495287No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17110.185485313487525No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17020.184509645561524No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16830.18244990216218854No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16750.181582641783521No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA16120.1747529663015139No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16030.1737772983755129No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC15970.17312685309151224No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15920.17258481535484502No Hit
CTTCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTT15870.17204277761817777No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAAGTATGTCTCCTGGTACCAACAGCA15350.16640558515683862No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15250.16532150968350418No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14840.1608768002428329No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14650.15881705684349745No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14120.15307145683482484No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14070.15252941909815762No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13340.1446156681428161No Hit
GTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGA13210.14320637002748132No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12570.1362682869981408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12550.13605147190347391No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12460.13507580397747293No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCGCGAAATTGTTGC12300.13334128322013777No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC12140.13160676246280265No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12000.1300890568001344No Hit
GTAATATTCAGCCTCATCCTCAGCCTGGAGCCCAGAGACACTCAGGGAGG11970.12976383415813408No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11830.12824612849546585No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11740.12727046056946484No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11640.1261863850961304No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11580.1255359398121297No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11570.12542753226479628No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT11210.12152486056079224No Hit
CCCCTGAGGGCCGCTTATTGACCTCATAAATCAGGAGTTTGGGGGCTTTGCCTGGGTGCTGTTGGTACCAGGAGA11170.12109123037145847No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT10840.11751378130945475No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10640.11534563036278585No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10490.11371951715278418No Hit
GTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC10160.11014206809078049No Hit
GGTTATAAGTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTCAATAAG10110.10960003035411325No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCAC10040.10884117752277914No Hit
CACTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGAGCAGGGACCCCTGAGGGCCGCTTATTGACCT10000.10840754733344535No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCA9760.10580576619744267No Hit
CCTCAGGGGTCCCTGCTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGTGTCTCTGGGCTCCAGG9730.10548054355544233No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9560.10363761525077377No Hit
GGATGAGGCTGAATATTACTGCAGCTCATATGCAGGCAGCAACAATTTCG9390.10179468694610518No Hit
GTCAATAAGCGGCCCTCAGGGGTCCCTGCTCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGTGT9360.10146946430410486No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTTCTGGACAGTCAGTCACCATCT9320.10103583411477106No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9240.10016857373610351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTACG200.00780137350.9907261
GGTATCA9050.044.3234251
CTAATCC350.001454647238.8205954
GTATCAA22050.034.3792341
TGGTATC2600.033.980912
GTACGTA550.00411415531.40433169
TATCAAC24450.030.9928152
TCCGAGA2701.0913936E-1130.07965770
ATCAACG25350.029.7503663
TCAACGC25250.029.7304234
AACGCAG25500.029.5721576
GTGGTAT3350.029.427481
CAACGCA25750.029.1531355
ACGCAGA27450.027.7188937
CGCAGAG28000.027.05318
AGAGTAC28150.027.02961311
TGGTACG550.00867834826.95567567
AATGGTA2805.838956E-1026.58826870
CAGAGTA28850.026.37378110
GCAGAGT29050.026.0752779
Produced by FastQC (version 0.11.5)