FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164717
Sequences flagged as poor quality0
Sequence length20-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT8080.4905383172350152No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.40008013744786514No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.3921878130369057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.348476477837746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.3369415421601899No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.2513401773951687No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4130.25073307551740254No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT3820.2319129173066532No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3770.22887740791782268No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.22402059289569382No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3450.2094501478293072No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.18880868398525957No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.18820158210749344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.1845589708408968No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT2950.17909505394100184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2940.17848795206323573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.16513171075238137No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC2630.1596677938524864No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2590.15723938634142196No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.15663228446365585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.15663228446365585No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.1542038769525914No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.1523825713192931No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.14873996005269643No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG2450.14873996005269643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.1450973487860998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.14206183939726927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2340.14206183939726927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2270.1378121262529065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.1372050243751404No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2220.13477661686407597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.13356241310854375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.12688429245311655No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2080.12627719057535045No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.11777776428662495No Hit
GTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTA1880.11413515302002829No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1870.11352805114226219No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1820.11049254175343165No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.10988543987566553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1800.10927833799789942No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.10624282860906889No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1750.10624282860906889No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.10320731922023835No Hit
GTATCAACGCAGAGTACGGGGATCGAAAGATTTCCGCATCCCCGAAAGGG1690.10260021734247224No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)