FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164717
Sequences flagged as poor quality0
Sequence length20-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41822.5389000528178634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.4407559632581943No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.43043523133617057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.3849025905037124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.3296563196269966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4870.29565861447209457No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.2452691586175076No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3990.24223364922867704No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3790.2300916116733549No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3560.21612826848473443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.21248565721813778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.20520043468494448No Hit
GAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGT3260.19791521215175117No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.19548680464068677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3160.19184419337409012No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3090.18759448022972736No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.18577317459642903No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.1833447670853646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2950.17909505394100184No Hit
ACCTAACGCCATGTACCCTTTCGGGGATGCGGAAATCTTTCGATCCCCCGTACTCTGCGTTGATAC2880.17484534079663908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2730.16573881263014745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2700.16391750699684915No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.16209620136355082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.15845359009695417No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGGGTACATGGCGTTAGGT2600.15784648821918806No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.15541808070812363No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2480.15056126568599476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.1450973487860998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.14327604315280146No Hit
GTATCAACGCAGAGTACGGGGGATCGAAAGATTTCCGCATCCCCGAAAGG2290.13902633000843873No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTT2280.1384192281306726No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2260.1372050243751404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.13599082061960818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.13295531123077764No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1980.12020617179768937No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1970.11959906991992326No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1900.11534935677556052No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1830.11109964363119776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1820.11049254175343165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.10684993048683501No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1730.10502862485353666No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.10199311546470614No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1680.10199311546470614No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.10077891170917391No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGTA156.754885E-494.44906662
GAAGCTC250.003578023862.04771867
ACTGTAC150.00449752158.5859643
TAGCCTC150.004508353358.5503925
TGTGACA200.007877639550.84314737
CCACTAC250.008478573549.90038753
ACCTAAC2350.046.1769751
CTAACGC2450.041.847123
CCTAACG2500.041.022642
TTATTGT500.001191672540.39057566
CGGGAGA300.001412670139.0335966
CATCCCG455.7277066E-639.03359611
TAACGCC2650.038.6653564
AACGCCA2900.035.3321345
CGGGACC601.043627E-634.1647717
GTACGGA350.0029835733.518421
TAGAACA350.003010363833.457374
TAGTCTC350.003010363833.457377
CTGTATC452.5923643E-432.5873531
ACGCCAT3200.032.019756
Produced by FastQC (version 0.11.5)