FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567016
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGACCGACAGGCTGTTCATTTGCAGATACA96970.6188194632345809No Hit
GTATATTACTGTGTGAGGGGACTTTTTTTTCTTTATAGGCCTGGTTTGTA59050.37683086835105706No Hit
CTATACAGACTCCGTGAAGGGCCGATTTACCATCTCCAGCGACACTTCCAAGGGCACGCTGTATCTGCAAATGAA54510.34785860514506556No Hit
GAGTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGGAAGTCCCTAAACTCCTCATCTTTGAGGCGT46980.299805490180062No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45910.29297722550376004No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44240.28232002736411116No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA36690.23413928128366268No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34850.22239721866273224No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG31570.20146571572976918No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT31010.19789204449731207No Hit
CAGTAATATACAGCCGTGTCCTCGACCGACAGGCTGTTCATTTGCAGATA30690.19584994665019376No Hit
CTCCTGTGCAGCCTCTGCATCCACCCTCTCCAGTTATGTCATGCACTGGG30080.1919571976291244No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30070.19189338207140197No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29060.18544801074143466No Hit
ATATAATGTTTATCCGTGGACGTTCGGCCTAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28840.1840440684715408No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC27810.17747106602612864No Hit
GTCTGTATAGTATGCAGTCTTGCCATCATGCCACATAAGTGCCACCCACT27190.17351450144733685No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26670.17019609244576955No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26560.16949412131082262No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC26550.16943030575310014No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26480.16898359684904302No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26320.16796254792548385No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG25990.16585663452064306No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25080.16004941876790027No Hit
GAATTCACTCTCACCATCAGCAGCCTTCTACCTGATGATTTTGCAACTTATTACTGTCAACAATATAATGTTTAT24880.1587731076134513No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24620.15711390311266762No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23980.15302970741843097No Hit
ATACTATACAGACTCCGTGAAGGGCCGATTTACCATCTCCAGCGACACTT23790.15181721182170443No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC23680.1511152406867575No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23510.15003037620547588No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23410.14939222062825142No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23020.14690341387707592No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA23000.14677578276163103No Hit
CCTTCCACCCTGTCTGCATATGTGGGCGACAGCGTCACCCTCACTTGCCG22960.14652052053074124No Hit
ACATTATATTGTTGACAGTAATAAGTTGCAAAATCATCAGGTAGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC22730.14505276270312492No Hit
GTATAGTATGCAGTCTTGCCATCATGCCACATAAGTGCCACCCACTCCGGCCCCTTGCCTGGAGCCTGGCGGACC22350.1426277715096719No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22320.14243632483650454No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22310.1423725092787821No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT22190.1416067225861127No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC21930.13994751808532904No Hit
GTTTAGGGACTTCCCCTGGTTTCTGCTGATACCAGGCCAACCACTTACTA21920.13988370252760662No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21860.13950080918127192No Hit
GTCCCTAAACTCCTCATCTTTGAGGCGTCTGAGTTAGTAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGAT21430.13675674019920664No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG21170.13509753569842298No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20950.13369359342852913No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20680.13197057337002302No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT20600.13146004890824345No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20510.1308857088887414No Hit
GGTTTGTACCCTTTGGCCCTCTGGGGCCAGGGAACCCAGGTCACCGTCTC20470.13063044665785162No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC20290.12948176661884755No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGAGGGCCAAAGGGTACAAAC20230.12909887327251285No Hit
ATATTACTGTGTGAGGGGACTTTTTTTTCTTTATAGGCCTGGTTTGTACCCTTTGGCCCTCTGGGGCCAGGGAAC20230.12909887327251285No Hit
GCCTAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC20230.12909887327251285No Hit
GTCTGCATATGTGGGCGACAGCGTCACCCTCACTTGCCGGGCCAGTCAGA20210.12897124215706796No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT20090.12820545546439857No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20050.12795019323350879No Hit
TCTTTGAGGCGTCTGAGTTAGTAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT19310.1232278419620476No Hit
GAGTTAGTAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC19310.1232278419620476No Hit
GTACAAACCAGGCCTATAAAGAAAAAAAAGTCCCCTCACACAGTAATATACAGCCGTGTCCTCGACCGACAGG19180.1223982397116558No Hit
GTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGGAAGTCCCT18940.12086666632631703No Hit
GCCTTCTACCTGATGATTTTGCAACTTATTACTGTCAACAATATAATGTTTATCCGTGGACGTTCGGCCTAGGGA18860.12035614186453744No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC18830.12016469519137009No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGT18800.11997324851820276No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18670.11914364626781093No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18610.11876075292147623No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT18300.11678247063208033No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18210.1162081306125783No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18030.11505945057357424No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17970.11467655722723955No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG17800.11359169274595793No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAAATGACCCAGTCTCCTTCCACC17800.11359169274595793No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17640.11257064382239874No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17350.11071999264844776No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT17110.10918841926310899No Hit
CTTCCAAGGGCACGCTGTATCTGCAAATGAACAGCCTGTCGGTCGAGGAC17070.1089331570322192No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACA16930.10803973922410492No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT16870.10765684587777025No Hit
CTCCAGTTATGTCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCCGG16740.10682724362737842No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16700.1065719813964886No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGG16650.10625290360787637No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16450.1049765924534274No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16440.10491277689570494No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC16430.10484896133798251No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC16420.10478514578026006No Hit
ACTTATTACTGTCAACAATATAATGTTTATCCGTGGACGTTCGGCCTAGGGACCAAGGTGGAAATCAAACGAA16310.10408317464531314No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTAGGCCGAACGTC16200.1033812035103662No Hit
GCACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA15850.10114765899008052No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT15790.10076476564374581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAACG252.6521282E-454.8386652
ATCTCGT2350.049.898737
CGTATGC2600.049.24629241
TCGTATG2750.047.78617540
ATGCCGT2700.047.53263544
GCCGTCT2750.046.7408746
ATCTCCG2950.046.46751410
AATCTCG2550.045.95850836
CGAGACT3000.045.70034823
ACGAGAC3000.045.69743322
TCTCCGA3000.044.5507311
CTAATCT2800.041.80944434
CTTATAC3400.041.3424871
CCCACGA3300.040.50066819
TACACAT3650.040.3777855
CCGTCTT3200.040.1978447
CACGAGA3500.040.1471821
GCTAATC3000.040.14154433
GTGGTAT2500.039.769461
TTATACA3600.039.0344732
Produced by FastQC (version 0.11.5)