FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567016
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCGACCGACAGGCTGTTCATTTGCAGATACA88000.5615769079575448No Hit
GTATATTACTGTGTGAGGGGACTTTTTTTTCTTTATAGGCCTGGTTTGTA55160.3520066163970247No Hit
CTATACAGACTCCGTGAAGGGCCGATTTACCATCTCCAGCGACACTTCCAAGGGCACGCTGTATCTGCAAATGAA49760.3175462152269026No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT46570.2971890523134416No Hit
GAGTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGGAAGTCCCTAAACTCCTCATCTTTGAGGCGT42160.2690463913578419No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41570.26528127345221747No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36380.23216099899426684No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33760.21544132287098536No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA30230.1929144309949611No Hit
CAGTAATATACAGCCGTGTCCTCGACCGACAGGCTGTTCATTTGCAGATA29320.18710721524221832No Hit
CTCCTGTGCAGCCTCTGCATCCACCCTCTCCAGTTATGTCATGCACTGGG29300.18697958412677343No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT29110.18576708853004692No Hit
ATATAATGTTTATCCGTGGACGTTCGGCCTAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT28720.18327828177887143No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27740.17702435712207149No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27070.17274871475466747No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26100.16655860565559No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26010.16598426563608795No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25940.1655375567320308No Hit
GTCTGTATAGTATGCAGTCTTGCCATCATGCCACATAAGTGCCACCCACT25750.1643250611353043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25460.1624744099613533No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC24660.15736916534355744No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24140.1540507563419901No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA24140.1540507563419901No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24060.15354023188021054No Hit
ACATTATATTGTTGACAGTAATAAGTTGCAAAATCATCAGGTAGAAGGCTGCTGATGGTGAGAGTGAATTCTGTC23570.15041326955181059No Hit
GAATTCACTCTCACCATCAGCAGCCTTCTACCTGATGATTTTGCAACTTATTACTGTCAACAATATAATGTTTAT23550.15028563843636566No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23140.1476692005697453No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23020.14690341387707592No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22950.14645670497301877No Hit
GTTTAGGGACTTCCCCTGGTTTCTGCTGATACCAGGCCAACCACTTACTA22610.14428697601045554No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC21980.1402665958739413No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21830.13930936250810458No Hit
ATACTATACAGACTCCGTGAAGGGCCGATTTACCATCTCCAGCGACACTT21490.13713963354554135No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21100.13465082679436585No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC20760.1324810978318026No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20390.13011992219607202No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20340.1298008444074598No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20330.12973702884973734No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC20320.1296732132920149No Hit
GTATAGTATGCAGTCTTGCCATCATGCCACATAAGTGCCACCCACTCCGGCCCCTTGCCTGGAGCCTGGCGGACC20240.1291626888302353No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGGCCCCAGAGGGCCAAAGGGTACAAAC19890.12692914430994962No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19830.1265462509636149No Hit
ATATTACTGTGTGAGGGGACTTTTTTTTCTTTATAGGCCTGGTTTGTACCCTTTGGCCCTCTGGGGCCAGGGAAC19790.12629098873272515No Hit
GGTTTGTACCCTTTGGCCCTCTGGGGCCAGGGAACCCAGGTCACCGTCTC19720.12584427982866797No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC19700.12571664871322308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19430.123993628654717No Hit
CCTTCCACCCTGTCTGCATATGTGGGCGACAGCGTCACCCTCACTTGCCG19420.12392981309699454No Hit
GTCCCTAAACTCCTCATCTTTGAGGCGTCTGAGTTAGTAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGAT19210.12258968638482312No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19160.12227060859621088No Hit
GAGTTAGTAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC18840.12022851074909255No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18820.12010087963364766No Hit
GTATTAGTAAGTGGTTGGCCTGGTATCAGCAGAAACCAGGGGAAGTCCCT18580.11856930624830889No Hit
GCCTAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTC18580.11856930624830889No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18450.11773970399791706No Hit
GTCTGCATATGTGGGCGACAGCGTCACCCTCACTTGCCGGGCCAGTCAGA18290.1167186550743579No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18210.1162081306125783No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG18210.1162081306125783No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT18210.1162081306125783No Hit
GCCTTCTACCTGATGATTTTGCAACTTATTACTGTCAACAATATAATGTTTATCCGTGGACGTTCGGCCTAGGGA18200.11614431505485585No Hit
TCTTTGAGGCGTCTGAGTTAGTAAGTGGGGTCCCATCACGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT18160.11588905282396607No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18060.11525089724674159No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18020.11499563501585178No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18010.11493181945812934No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17830.11378313941912527No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC17790.11352787718823547No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17770.11340024607279058No Hit
GTACAAACCAGGCCTATAAAGAAAAAAAAGTCCCCTCACACAGTAATATACAGCCGTGTCCTCGACCGACAGG17680.11282590605328854No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGT17420.1111667015525049No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG17320.1105285459752804No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17170.1095713126094437No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT17080.10899697258994163No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCGTGATGGGACCCCA16810.10727395253143554No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTAGGCCGAACGTC16790.10714632141599065No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16680.10644435028104371No Hit
CTTCCAAGGGCACGCTGTATCTGCAAATGAACAGCCTGTCGGTCGAGGAC16550.10561474803065188No Hit
ACTTATTACTGTCAACAATATAATGTTTATCCGTGGACGTTCGGCCTAGGGACCAAGGTGGAAATCAAACGAA16210.10344501906808866No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCTGGGTTCCCTGG16190.10331738795264374No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC16140.1029983101640315No Hit
GGGCCGGAGTGGGTGGCACTTATGTGGCATGATGGCAAGACTGCATACTA16050.10242397014452947No Hit
CTCCAGTTATGTCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCCGG15980.10197726124047235No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG15860.10121147454780295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)