FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279254
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC81190.6346667667249819No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC55380.4329085545169294No Hit
CCCATACACCCAGCAGCGCGGGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCCAAGGCTGCCC50750.39671558580235045No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG49670.3882731654542413No Hit
ACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTGCTGG49050.38342659080995645No Hit
GTGTATGGGAGTAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGC45690.35716128306028355No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC45570.3562232363549381No Hit
GCCTTGGGCTGACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTGCTGGGTGTATGGGAGTAG44380.3469209398602623No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43560.34051095404040166No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT39760.31080614170446214No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT37550.2935304482143499No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC36360.2842281517196741No Hit
GATTATTACTGCTACTCCCATACACCCAGCAGCGCGGGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGT35480.27734914254714077No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACCGTAAGCTTGGTCCCTC34670.2710173272860589No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC33520.26202771302649824No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA33500.26187137190894066No Hit
GTAATAAACAGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATCCA32020.25030212920968004No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31590.2469407951821921No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA31120.2432667789195891No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30910.2416251971852345No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30740.2402962976859951No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30440.23795118092263148No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTACTCCCAT29500.2306031483974254No Hit
GTCCCGCGCTGCTGGGTGTATGGGAGTAGCAGTAATAATCAGCCTCGTCC29340.22935241945696477No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG28690.22427133313634354No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27730.21676695949357985No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27530.21520354831800406No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26320.20574491070577072No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT25770.20144552997293733No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG25720.20105467717904343No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT25670.2006638243851495No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25500.19933492488591006No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC24720.1932376213011646No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC24680.19292493906604943No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24500.19151786900803125No Hit
GCGCGGGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCC23560.18416983648282514No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23060.18026130854388575No Hit
GTTTATTACTGTGCGAGAGGTTGGAGAGCAGCAGTCCACCTTTCTGCTTT22360.17478936942937057No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21830.17064632981409478No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21520.16822304249195236No Hit
GACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTGCTGGGTGTATGGGAGTAGCAGTAATAAT21370.16705048411027051No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20980.16400183231789778No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20590.160953180525525No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA20440.1597806221438432No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20390.15938976934994925No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19060.1489930850323704No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT18620.14555358044610375No Hit
GTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18610.14547540988732496No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACCG18580.1452408982109886No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18520.14477187485831586No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG18340.14336480480029767No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17870.13969078853769462No Hit
GGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA17630.13781469512700373No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT16940.13242092657126733No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG16890.13203007377737339No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC16560.12945044533767336No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16330.12765252248576123No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15960.12476021181094606No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTACTCCCATACACCCAGCAGCGCGGGA15770.1232749711941491No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT15660.12241509504758241No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15610.12202424225368846No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15510.1212425366659006No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT15180.11866290822620058No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15150.11842839654986421No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15140.11835022599108543No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAATCGGCCCTCAGGGGTTTCTAATCG15020.11741217928573996No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT14860.11616145034527936No Hit
CCCCTGAGGGCCGATTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA14790.11561425643382783No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14780.11553608587504904No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTG14700.11491072140481876No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC14490.11326913967046419No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14130.11045499955442781No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA13970.1092042706139672No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG13900.10865707670251568No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC13760.10756268887961264No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13750.10748451832083385No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13750.10748451832083385No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13710.10717183608571872No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGT13560.10599927770403687No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13340.10427952541090355No Hit
GGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAATCGGCCCTCAGG13310.10404501373456718No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13280.10381050205823081No Hit
CGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13230.10341964926433686No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13220.10334147870555807No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC13200.10318513758800052No Hit
CTCCTGTGCAGCGTCTGGATTCACCTTCACTAACTATGGCATACACTGGGTCCGCCAGGCTCCAGGCAAGGGG13150.10279428479410657No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC13150.10279428479410657No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13100.10240343200021262No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA12960.10130904417730958No Hit
CGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC12860.10052733858952172No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG12840.10037099747196414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)