FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1279254
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC74470.5821361512256362No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC51700.4041417888863353No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48110.37607855828475034No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC48070.3757658760496352No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG47960.3749059999030685No Hit
GTGTATGGGAGTAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGC45870.35856835311830176No Hit
GCCTTGGGCTGACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTGCTGGGTGTATGGGAGTAG45500.3556760424434866No Hit
ACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTGCTGG43370.3390257134236047No Hit
CCCATACACCCAGCAGCGCGGGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCCAAGGCTGCCC42570.3327720687213016No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38210.2986897050937499No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT36710.2869641212769317No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36450.2849316867486832No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC35340.2762547547242377No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34940.2731279323730862No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC32570.2546015099425134No Hit
GATTATTACTGCTACTCCCATACACCCAGCAGCGCGGGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGT32570.2546015099425134No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAACAACACCCAGGCAAAGC30930.2417815383027921No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT30740.2402962976859951No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA30670.2397491037745436No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACCGTAAGCTTGGTCCCTC30610.23928008042187085No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG30290.23677862254094964No Hit
GTCCCGCGCTGCTGGGTGTATGGGAGTAGCAGTAATAATCAGCCTCGTCC30020.23466801745392235No Hit
GTAATAAACAGCCGTGTCCTCGCCTCTCAGGCTGTTCATTTGCAGATCCA29600.23138485398521325No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAATTATGTCTCCTGGTACCAACAACA29250.22864888442795567No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG29240.22857071386917688No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28820.2252875504004678No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTACTCCCAT26940.2105914853500556No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26250.2051977167943192No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25610.20019480103247675No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG25030.19566090862330704No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA24520.19167421012558883No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24360.1904234811851282No Hit
GCGCGGGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCC24100.18839104665687972No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG23730.18549873598206454No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG23580.18432617760038272No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACCG23460.18338813089503725No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23270.1819028902782403No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23030.18002679686754938No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22280.17416400495914025No Hit
GTTTATTACTGTGCGAGAGGTTGGAGAGCAGCAGTCCACCTTTCTGCTTT21730.1698646242263069No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA21030.16439268511179173No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20310.15876440487971896No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19730.15423051247054922No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19620.15337063632398257No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19490.15235441905985833No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC19120.14946210838504315No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18800.14696065050412194No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18790.14688247994534315No Hit
GTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC18790.14688247994534315No Hit
GACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTGCTGGGTGTATGGGAGTAGCAGTAATAAT18310.1431302931239613No Hit
GGACTGTTCGGCGGAGGGACCAAGCTTACGGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCA17800.13914359462624312No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17730.1385964007147916No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17520.13695481898043704No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC17330.13546957836364004No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17010.13296812048271883No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAG16660.13023215092546123No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG16220.12679264633919457No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT15990.12499472348728242No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT15650.12233692448880362No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG15200.11881924934375816No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC15190.11874107878497937No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGTCCCGCGCTG15030.11749034984451875No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC14860.11616145034527936No Hit
GCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCC14820.1158487681101642No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14700.11491072140481876No Hit
GACCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTACTCCCATACACCCAGCAGCGCGGGA14600.11412901581703086No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14290.11170572849488843No Hit
CCCCTGAGGGCCGATTATTGACATCATAAATCATGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA14140.1105331701132066No Hit
GGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACCGTAAGCTTGGTCCCTCCGCCGAACAGT14110.11029865843687023No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13960.10912610005518841No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13830.10810988279106416No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC13800.1078753711147278No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13640.10662464217426719No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGC13470.1052957426750278No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG13410.10482671932235506No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA13350.10435769596968233No Hit
GAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT13260.10365416094067323No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAAC12890.10076185026585806No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCATGATTTATGATGTCAATAATCGGCCCTCAGGGGTTTCTAATCG12870.1006055091483005No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA200.00775062851.076129
GAGTTAG200.00785578950.902271
GTATAGC200.00785578950.902271
AAAACCG1206.1390892E-945.52785570
ATACGAT306.7055086E-445.4384330
ACAATGG8700.043.9579370
GGTATCA13350.042.1961441
GTATCAA32450.037.1244131
GTGGTAT4550.036.545221
TCTGGAG7900.033.71366570
TGGTATC5150.032.934862
TATCAAC38200.031.4364132
AATGGTA3700.031.37730470
TAGTACT553.6032475E-430.828164
ATCAACG39150.030.5785683
AACGCAG40050.030.0584146
TGTGCGA7000.030.0354310
TCAACGC40050.029.889074
CAACGCA40500.029.556975
GTGCGAG7350.029.06654511
Produced by FastQC (version 0.11.5)