FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819948
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTTCATGGAGA46480.5668652158429559No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT31110.3794143043217375No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29780.3631937635069541No Hit
GTCTATTACTGTGCGAGCCCCTCTTGTAGTGGTACCAGTTGCTATGCCAT29060.35441271885534203No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25030.3052632605970135No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21320.2600164888505125No Hit
GTATGGTAGCTCACCTGAGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21020.2563577202456741No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19720.24050305629137458No Hit
GTCTCTGGTGGCTCCATCAGCAGAAGTAGTTACTACTGGGGCTGGATCCG19230.23452706757013858No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17870.21794064989487139No Hit
GTATTTCTGTCAGCAGTATGGTAGCTCACCTGAGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA17410.21233053803411925No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17250.21037919477820544No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16920.20635454931288325No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT16610.2025738217545503No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16130.19671979198680892No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16070.19598803826584127No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15420.18806070628869148No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT15420.18806070628869148No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14760.1800114153580471No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT14540.17732831838116564No Hit
CTCTTATAGTGGGAACACCCACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAGGAA14510.1769624415206818No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14500.17684048256718718No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14150.17257191919487577No Hit
GCACAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT13680.166839848380629No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13670.16671788942713442No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTTCTGTCAGCAGTATGGTAGCTCA13560.1653763409386937No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC13420.1636689155897691No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT13360.16293716186880144No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGAAGTAGTTACTACTGGG13180.16074190070589842No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG13090.15964427012444693No Hit
GTGTATTTCTGTCAGCAGTATGGTAGCTCACCTGAGTGGACGTTCGGCCA12990.1584246805895008No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12980.1583027216360062No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA12980.1583027216360062No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12890.15720509105455466No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTTCATGGA12500.15244869186826482No Hit
CTACAAGAGGGGCTCGCACAGTAATAGACAGCCGTGTCTGCGGCGGTCAC12410.1513510612868133No Hit
GTCAGAGTGTTAGCAGCAACTTCTTAGCCTGGTACCAGCACAAACCTGGC12390.1511071433798241No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA12310.1501314717518672No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12290.14988755384487798No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12170.14842404640294263No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11540.14074063233278208No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11480.1400088786118144No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACTCAGGTGAGCTACCATACTG11480.1400088786118144No Hit
CTACCATACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11060.1348866025650407No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCA11020.13439876675106224No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11020.13439876675106224No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10960.1336670130300946No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10810.1318376287276754No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTCTTAGCCTGGTACC10710.1306180391927293No Hit
GAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10590.12915453175079394No Hit
CAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10580.12903257279729935No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC10540.1285447369833209No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10490.12793494221584784No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10430.12720318849488016No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10370.12647143477391248No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10270.12525184523896638No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT10190.12427617361100947No Hit
CTCTTGTAGTGGTACCAGTTGCTATGCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC10010.12208091244810645No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA9980.12171503558762262No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC9950.12134915872713879No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9900.12073936395966572No Hit
GGTTTGTGCTGGTACCAGGCTAAGAAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC9850.12012956919219267No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9610.11720255430832198No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9590.11695863640133278No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC9550.11647080058735432No Hit
CTATAAGAGATACTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTACTT9480.11561708791289205No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGGCATAGCAA9170.11183636035455907No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9060.11049481186611834No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGAAGTTGCTGCTAACACTCTGA9030.1101289350056345No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC8970.10939718128466683No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8940.10903130442418299No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA8790.10720192012176383No Hit
GGACTGGAGTGGATTGGGAGTATCTCTTATAGTGGGAACACCCACTACAA8750.10671408430778537No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8700.10610428954031231No Hit
CTATTACTGTGCGAGCCCCTCTTGTAGTGGTACCAGTTGCTATGCCATTGACTACTGGGGCCAGGGAACCCTGGT8600.1048847000053662No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC8580.10464078209837697No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8510.10378706942391469No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8490.10354315151692546No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT8430.10281139779595778No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8360.10195768512149551No Hit
GTCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGAAGTTGCTG8220.10025025977257093No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACC8210.10012830081907634No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGT8200.10000634186558173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)