FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819948
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTTCATGGAGA42210.5147887427007567No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32970.4020986696717353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28720.3502661144365253No Hit
GTCTATTACTGTGCGAGCCCCTCTTGTAGTGGTACCAGTTGCTATGCCAT27420.3344114504822257No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23670.2886768429217462No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA22670.2764809475722851No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21280.259528653036534No Hit
GTCTCTGGTGGCTCCATCAGCAGAAGTAGTTACTACTGGGGCTGGATCCG19850.24208852268680453No Hit
GTATGGTAGCTCACCTGAGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT19360.23611253396556856No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18360.22391663861610736No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT18020.21977003419729058No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17690.21574538873196836No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16830.20525691873143176No Hit
GTATTTCTGTCAGCAGTATGGTAGCTCACCTGAGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA16410.20013464268465805No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15990.19501236663788435No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14850.18110904593949861No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14720.17952357954406864No Hit
CTCTTATAGTGGGAACACCCACTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCAGGAA14530.17720635942767102No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14190.1730597550088542No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA14070.17159624756691888No Hit
GTGTATTTCTGTCAGCAGTATGGTAGCTCACCTGAGTGGACGTTCGGCCA13970.17037665803197277No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13410.1635469566362745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13380.16318107977579066No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTTCTGTCAGCAGTATGGTAGCTCA13140.16025406489191998No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13050.15915643431046847No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12860.15683921419407085No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12790.15598550151960858No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG12720.15513178884514628No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGAAGTAGTTACTACTGGG12460.15196085605428636No Hit
GCACAAACCTGGCCAGGCTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTT12370.15086322547283487No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12260.14952167698439414No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC12210.1489118822169211No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12170.14842404640294263No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12110.14769229268197495No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12070.14720445686799652No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11780.14366764721665276No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC11580.14122846814676052No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11310.137935576402406No Hit
GTCAGAGTGTTAGCAGCAACTTCTTAGCCTGGTACCAGCACAAACCTGGC11280.13756969954192216No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCACTCAGGTGAGCTACCATACTG11260.13732578163493295No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11210.13671598686745987No Hit
CTACCATACTGCTGACAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC11120.13561835628600838No Hit
CTACAAGAGGGGCTCGCACAGTAATAGACAGCCGTGTCTGCGGCGGTCAC11080.13513052047202995No Hit
CTCCCAGACTCCTCATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCA11060.1348866025650407No Hit
CAGTAATAGACAGCCGTGTCTGCGGCGGTCACAGACCTCAACTTCATGGA10940.13342309512310535No Hit
CTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGTCCA10870.13256938244864308No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10830.13208154663466462No Hit
CTCTTGTAGTGGTACCAGTTGCTATGCCATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATC10610.1293984496577832No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG10450.12744710640186938No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA10440.12732514744837478No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10300.1256177220994502No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTCTTAGCCTGGTACC10290.1254957631459556No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT10270.12525184523896638No Hit
GGTTTGTGCTGGTACCAGGCTAAGAAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCC10040.1224467893085903No Hit
GAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9930.12110524082014956No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9880.12049544605267652No Hit
CAGAAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9870.1203734870991819No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC9770.11915389756423578No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT9730.11866606175025733No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC9330.11378770361047286No Hit
CTATTACTGTGCGAGCCCCTCTTGTAGTGGTACCAGTTGCTATGCCATTGACTACTGGGGCCAGGGAACCCTGGT9140.11147048349407523No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9000.10976305814515068No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8890.10842150965670994No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC8890.10842150965670994No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8870.10817759174972072No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGTAGTCAATGGCATAGCAA8870.10817759174972072No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8860.1080556327962261No Hit
GGACTGGAGTGGATTGGGAGTATCTCTTATAGTGGGAACACCCACTACAA8850.10793367384273149No Hit
CCATAGATGAGGAGTCTGGGAGCCTGGCCAGGTTTGTGCTGGTACCAGGCTAAGAAGTTGCTGCTAACACTCTGA8810.10744583802875303No Hit
CTATAAGAGATACTCCCAATCCACTCCAGTCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTAACTACTT8750.10671408430778537No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA8650.10549449477283925No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA8370.10207964407499012No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8320.10146984930751707No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8320.10146984930751707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCTA250.001293257180.2473470
TACGGAT8600.071.5383370
CGAACTA200.006895641352.6067652
CTACGGA8350.048.37275369
GGTATCA6900.043.0081861
GTATCAA19250.041.463511
TCTACGG9900.037.03886868
TATCAAC21650.036.2169882
ATCAACG22050.035.701533
AATGGTA3000.035.66548570
TCAACGC22300.035.148474
TGTCTAC9950.035.08382466
GTGTCTA9700.034.9269165
CAACGCA22500.034.838165
ACGTGTC9700.034.7097563
AACGCAG22750.034.305526
GACGTGT10050.033.4323862
ACCCTGG11200.032.83931470
GGACGTG10400.032.58840661
ACGCAGA24150.032.4579167
Produced by FastQC (version 0.11.5)