FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393252
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT57610.41349303643561963No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA56260.403803475609581No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG48760.34997258213158855No Hit
GATTATTACTGCTGCTCATACACAACCAGTAACAGTCGAGTCTTCGGGACTGGGACCACGGTCACCGTCCTACGT48480.3479628954417435No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG46110.3309523331026979No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC45030.32320068444186695No Hit
GTCCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATACACAACCAGTAACAGTCGAGT43540.31250628027090577No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC41050.2946344236362123No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT38770.27826983201890254No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35520.25494311151177246No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34130.2449664525871845No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTTTAAATATGTCTCCTGGTACCAACAACA33460.24015755943648387No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTTTAAATATGTCTCCTGGTACCAACAACACCCAGGCAAAGC33050.2372148039263536No Hit
ACCAGTAACAGTCGAGTCTTCGGGACTGGGACCACGGTCACCGTCCTACG31580.2266639488046671No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGACAGGGAGG31290.22458248759018468No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT31130.2234340951959875No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC30160.21647196630616714No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29780.21374453436994886No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29150.2092227393177975No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28700.20599288570911797No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTA28670.20577756213520595No Hit
GAGATGGACAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT28610.20534691498738206No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26380.18934119599325894No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC25490.18295326330053716No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC25320.18173309638170265No Hit
GTTACTGGTTGTGTATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGACAGGGAGGC24900.1787185663469351No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT24040.17254595722812527No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23900.17154111388320276No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23210.16658867168322744No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC22720.1630717199759986No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC22620.16235397472962537No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA21950.15754508157892472No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT21690.1556789439383543No Hit
ACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTACTGG21680.15560716941371697No Hit
GACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTACTGGTTGTGTATGAGCAGCAGTAATAAT21310.152951512002136No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20910.15008053101664307No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20260.14541518691521707No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20070.14405147094710793No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19940.1431184021268227No Hit
GCCTTGGGCTGACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTACTGGTTGTGTATGAGCAG19310.13859660707467134No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19190.13773531277902346No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC19170.13759176372974882No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC19070.13687401848337558No Hit
GTAATCGGCCCTCTGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC18730.1344336846457066No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17910.12854817362544607No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17580.12617961431241442No Hit
GTTTTAAATATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA17540.12589251621386513No Hit
CTTCGGGACTGGGACCACGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC17420.12503122191821725No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG17010.12208846640808697No Hit
CCCCAGAGGGCCGATTACTGACCTCATAAATCACGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA16710.11993523066896729No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC16410.11778199492984759No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT16330.11720779873274899No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG16120.1157005337153652No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG15760.11311665082842155No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15580.11182470938494973No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15500.11125051318785116No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC15450.11089164056466454No Hit
GTCCCGAAGACTCGACTGTTACTGGTTGTGTATGAGCAGCAGTAATAATC15430.11074809151538989No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAC15410.11060454246611526No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15370.11031744436756595No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15290.10974324817046738No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15270.10959969912119272No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCGTGATTTATGAGGTCAGTAATCGGCCCTCTGGGGTTTCTAATCG15080.10823598315308357No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15060.10809243410380893No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15030.10787711052989697No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG14930.10715936528352372No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14740.10579564931541459No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14660.105221453118316No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14630.10500612954440403No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC14600.10479080597049206No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC14580.1046472569212174No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT14060.1009149816400766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)