FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1393252
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55240.39648247409657406No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT53790.38607516802416214No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC51740.3713613904735109No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA51050.3664089482735356No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG47610.3417185117982964No Hit
GATTATTACTGCTGCTCATACACAACCAGTAACAGTCGAGTCTTCGGGACTGGGACCACGGTCACCGTCCTACGT45900.3294450680853141No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC43480.3120756331230818No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG43000.3086304559404903No Hit
GTCCATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATACACAACCAGTAACAGTCGAGT42580.30561592590572273No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT37590.2698004381116984No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC34220.2456124233089204No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTTTAAATATGTCTCCTGGTACCAACAACA33100.2375736765495402No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTTTAAATATGTCTCCTGGTACCAACAACACCCAGGCAAAGC33050.2372148039263536No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC32550.23362607769448746No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC31510.2261615271322058No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG30830.22128085945686782No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30130.21625664273225517No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29280.21015580813808268No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGACAGGGAGG29200.20958161194098412No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTA28990.20807434692360033No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28010.20104044350914263No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG26990.19371944199613567No Hit
ACCAGTAACAGTCGAGTCTTCGGGACTGGGACCACGGTCACCGTCCTACG26670.19142265720774132No Hit
GAGATGGACAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATT24670.17706775228027663No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC24420.17527338916434354No Hit
GTTACTGGTTGTGTATGAGCAGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGACAGGGAGGC23420.16809593670061124No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC23240.16680399525713943No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT23100.1657991519122169No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22870.16414833784555846No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA22390.16070316066296694No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22180.15919589564558312No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21980.15776040515283668No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT21250.15252086485431207No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21030.15094182531229094No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20000.14354904927464665No Hit
GACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTACTGGTTGTGTATGAGCAGCAGTAATAAT19760.1418264606833509No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA19610.14074984281379105No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19570.14046274471524176No Hit
GCCTTGGGCTGACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTACTGGTTGTGTATGAGCAG19270.13830950897612207No Hit
ACGTAGGACGGTGACCGTGGTCCCAGTCCCGAAGACTCGACTGTTACTGG19240.13809418540221008No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC19040.1366586949094636No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18760.13464900821961856No Hit
GTAATCGGCCCTCTGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC18670.13400303749788264No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17940.12876349719935803No Hit
GTTTTAAATATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAA17570.12610783978777707No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC17300.12416992762256936No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16860.12101184853852713No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG16650.11950458352114335No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG16340.11727957325738632No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG16160.1159876318139145No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16130.11577230824000252No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT16070.11534166109217858No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15960.11455214132116805No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15920.11426504322261874No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15860.1138343960747948No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG15700.11268600368059761No Hit
CTTCGGGACTGGGACCACGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC15430.11074809151538989No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15420.11067631699075257No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15360.11024566984292863No Hit
CCCCAGAGGGCCGATTACTGACCTCATAAATCACGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGGTACCAGGAGA15260.10952792459655539No Hit
CATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGC15240.10938437554728075No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15210.10916905197336876No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15100.10837953220235823No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG14990.10759001243134766No Hit
GTCCCGAAGACTCGACTGTTACTGGTTGTGTATGAGCAGCAGTAATAATC14970.10744646338207303No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14940.10723113980816104No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCA14930.10715936528352372No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14720.10565210026613994No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATAC14530.1042883842980308No Hit
GCCTGGAGCCCAGAGATGGACAGGGAGGCCGTGTTGCCAGACTTGGAGCC14340.10292466832992164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)