FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242891
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTTACCCTTTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCAT101590.8173685383513115No Hit
CTCTTATACTGCTGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCCGTTGATGGTGAGAGTGAAATCTGTC91650.7373937054818162No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50990.4102531919532767No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45670.36744976027664533No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34720.2793487119948571No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT33820.2721075299442992No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAACTCCTGAT33620.27049837837750856No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA31410.25271725356447183No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28780.2315569104611748No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28690.230832792256119No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28550.22970638615936556No Hit
AGTCAGGACATTACCACTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAA27920.224637558723975No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT27870.22423527083227732No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26840.2159481402633055No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTACCA25410.2044427065607523No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25190.2026726398372826No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25000.2011439458488315No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24990.20106348827049195No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24940.20066120037879426No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24420.1964774063051386No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTACCACTGCTTTAGCCTGGTATCAGCAGAAA24280.1953510002083851No Hit
GTCTTCAGGCTGCAGGCCGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGAC23230.1869029544827342No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG22960.18473059986756682No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22890.18416739681919012No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT22180.17845490875708328No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22070.17756987539534844No Hit
GTATATTACTGTGCCAAAAATAACGGTTATTGGACTGGTTGGGGCCAGGG21940.1765239268769345No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21820.17555843593686013No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT19840.15962783542563266No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT18830.15150162001333986No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18210.14651325015628885No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17780.14305357428768894No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17630.14184671061259596No Hit
GATTTCACTCTCACCATCAACGGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGTCAGCAGTATAAGAGTTAC17370.1397548135757681No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17280.1390306953707123No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17130.13782383169561932No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTGAAAGGGTAACT16890.13589284981547053No Hit
GGACATTACCACTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAACTCCTGATCTATGATGCCTC16750.1347664437187171No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16380.1317895133201544No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16090.12945624354830795No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15960.128410295029894No Hit
GCCTGAAGACTTTGCAACTTATTACTGTCAGCAGTATAAGAGTTACCCTTTCACTTTCGGCCCTGGGACCAAA15800.12712297377646148No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTG15750.12672068588476382No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15300.12310009485948487No Hit
CATTACCACTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTA14900.11988179172590357No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14830.11931858867752683No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14580.11730714921903851No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC14560.11714623406235945No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT14560.11714623406235945No Hit
GGCTAAAGCAGTGGTAATGTCCTGACTTGCCCGGCAAGTGATGGTGACTC14420.11601982796560599No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14310.11513479460387113No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14250.11465204913383394No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14180.11408884608545722No Hit
ACTTATTACTGTCAGCAGTATAAGAGTTACCCTTTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAA13840.11135328842191311No Hit
CTCCTGTGCAGCCTCTGGATTCAGGTTTAGAGGATATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13580.10926139138508525No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13520.10877864591504806No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13450.10821544286667134No Hit
GAGTTACCCTTTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT13420.10797407013165275No Hit
GCTTAGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGA13290.10692812161323881No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13230.10644537614320161No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13210.10628446098652254No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13150.10580171551648535No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13130.10564080035980628No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13090.10531897004644815No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT12930.10403164879301564No Hit
GGGTAACTCTTATACTGCTGACAGTAATAAGTTGCAAAGTCTTCAGGCTG12880.10362936090131797No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12830.10322707300962032No Hit
CTCCTAAACTCCTGATCTATGATGCCTCCAGTTTGCAAAGTGGGGTCCCG12790.10290524269626218No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12680.10202020933452731No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12590.10129609112947152No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTG350.005064815756.87282670
GTTGCGA200.007939490550.7658925
ATAGAGT200.00797621350.7066654
CTAACGC352.5638592E-548.292063
ATCTCGT4450.047.68923637
CGTATGC5400.045.34036341
TCGTATG5500.045.05053340
AATCTCG4650.044.8325936
GGCAATC4750.043.015333
ACGAGAC5700.042.71753322
GCAATCT4950.042.0061234
ATGCCGT5950.041.3648344
CCCACGA6000.041.13535719
TATACAC6250.041.1061973
CACGAGA6050.040.8003221
GTATGCC6200.040.10873842
TACACAT6450.039.8315935
AGGCAAT5950.039.45096632
TTATACA6450.039.307492
CGTCTTC6250.039.1884348
Produced by FastQC (version 0.11.5)