FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242891
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGAGTTACCCTTTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCAT96710.7781052401216196No Hit
CTCTTATACTGCTGACAGTAATAAGTTGCAAAGTCTTCAGGCTGCAGGCCGTTGATGGTGAGAGTGAAATCTGTC96050.7727950399512106No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55710.44822916892953607No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50030.40252926443268155No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC37510.30179637635158674No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36820.296244803446159No Hit
GCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAACTCCTGAT33360.2684064813406807No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTT32000.2574642506865043No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA29280.23557978937815144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28430.22874089521929117No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28410.22857998006261207No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27160.2185227827701705No Hit
GTCTTCAGGCTGCAGGCCGTTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGAC26610.2140976159614962No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25930.20862650063440802No Hit
AGTCAGGACATTACCACTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAA25160.202431267102264No Hit
GTCTCCATCCTCCCTGTCTGCGTCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTACCA24430.1965578638834781No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24340.19583374567842235No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23540.1893971394112597No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23470.18883393636288298No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCT22430.1804663482155716No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22420.18038589063723207No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGACATTACCACTGCTTTAGCCTGGTATCAGCAGAAA21560.17346653890003227No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21270.17113326912818583No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21210.17065052365814862No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20330.16357025676426976No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20320.16348979918593023No Hit
GTATATTACTGTGCCAAAAATAACGGTTATTGGACTGGTTGGGGCCAGGG20280.1631679688725721No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19950.1605128687873675No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19180.15431763525522352No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGG18810.1513407048566608No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT18610.14973155328987015No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18330.14747874109636325No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17170.13814566200897746No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCAT16910.1360537649721496No Hit
GATTTCACTCTCACCATCAACGGCCTGCAGCCTGAAGACTTTGCAACTTATTACTGTCAGCAGTATAAGAGTTAC16910.1360537649721496No Hit
GTGGGGTCCCGTCAAGGTTCAGCGGCAGTGGATCTGGGACAGATTTCACT16870.1357319346587915No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACCTTGACGGGACCCCA16590.13347912246528457No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTG15630.12575519494468942No Hit
GGACATTACCACTGCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCTCCTAAACTCCTGATCTATGATGCCTC15580.1253529070529918No Hit
GACCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15390.12382421306454064No Hit
GCCTGAAGACTTTGCAACTTATTACTGTCAGCAGTATAAGAGTTACCCTTTCACTTTCGGCCCTGGGACCAAA15370.12366329790786158No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGTTTGGTCCCAGGGCCGAAAGTGAAAGGGTAACT14980.12052545235261981No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14970.12044499477428029No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14940.1202036220392617No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14900.11988179172590357No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14800.11907721594250824No Hit
GGGTAACTCTTATACTGCTGACAGTAATAAGTTGCAAAGTCTTCAGGCTG14650.11787035226741524No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14600.11746806437571758No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14550.11706577648401993No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14300.1150543370255316No Hit
GCTCCTGGGGCTTCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGCCATCCAGTTGACCCAGTCTCCATCCTCC14110.11352564303708049No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA14000.11264060967534563No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13770.11079008537353638No Hit
CTCCTGTGCAGCCTCTGGATTCAGGTTTAGAGGATATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG13660.10990505201180151No Hit
ACTTATTACTGTCAGCAGTATAAGAGTTACCCTTTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAA13630.10966367927678293No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13620.10958322169844338No Hit
GAGTTACCCTTTCACTTTCGGCCCTGGGACCAAACTGGATATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT13580.10926139138508525No Hit
GGCTAAAGCAGTGGTAATGTCCTGACTTGCCCGGCAAGTGATGGTGACTC13560.10910047622840618No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13330.10724995192659693No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12930.10403164879301564No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12900.10379027605799704No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12860.1034684457446389No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12780.10282478511792265No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12490.1004915153460762No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12480.10041105776773668No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12450.10016968503271807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)