FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481578
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACACCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGAACTGTGGCTGCACCAT66040.4457409599764576No Hit
ATAGTACACCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGA47840.3228989631325519No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43340.29252594193488296No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42400.2861813552847032No Hit
CTATAATACTGATGACAGTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGT39090.26384031080375114No Hit
CCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC35180.23744953016310988No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGCCTCAGTAGCAGGTGCCGT32620.2201706558817693No Hit
GTGTACTATAATACTGATGACAGTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGT31920.21544596369546523No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30920.20869640342931656No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26620.17967329428487733No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26520.17899833825826247No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA26380.17805339982100166No Hit
ATTCAGGGGTGGGGACAGGCGGGCGCCTCAGTAGCAGGTGCCGTCCACCT26090.17609602734381855No Hit
ATAATAGACAGCCGTGTCAGCGACGGTCACAGAACTCAACCTCAGGGAGATCAAGTTCTGGGACGTGTCGGCCGA25990.1754210713172037No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG25540.17238376919743678No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25240.1703589011175922No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25230.17029140551493072No Hit
GTCTCTGGTGCCGCCATCAGCAGTGGTACTTCCTACTGGGGCTGGTTCCG24970.16853651984573206No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24120.16279939361950568No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23620.15942461348643136No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT23230.1567922849826334No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG23110.15598233775069556No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23000.1552398861214192No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22980.15510489491609622No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC22600.1525400620149597No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC22210.14990773351116174No Hit
ATATATTACTGTCATCAGTATTATAGTACACCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGAA22150.14950275989519282No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21980.14835533464994755No Hit
ATATTACTGTCATCAGTATTATAGTACACCGTACACTTTTGGCCAGGGGA21790.14707291819937932No Hit
CATCTGCACTGTCTCTGGTGCCGCCATCAGCAGTGGTACTTCCTACTGGG21620.14592549295413404No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21400.14444058969558135No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20790.14032335793323067No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC19510.13168392079256036No Hit
ACTATAACCCGTCTCTCAAGAGTCGAGTCACCATGTCGGCCGACACGTCC19360.13067148675263807No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19180.1294565659047313No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG19170.1293890703020698No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19130.12911908789142387No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19060.12864661867279348No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTCGGGCCAGTCAGACTATTAACAA18540.12513684733439615No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18500.12486686492375022No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA18340.12378693528116644No Hit
GTCTATTATTGTGCGAGACGCCGTTTGGTGGGAGCTATTCGACCCTTTGA18250.12317947485721305No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18080.12203204961196779No Hit
GTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGTCTGTCTCAGACCCACTGCCA17960.12122210238002995No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17900.12081712876406102No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17440.11771233104163263No Hit
CAGTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGTCTGTCTCAGACCCACTGCCA17350.11710487061767927No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT17160.11582245416711101No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17110.11548497615380358No Hit
GGACAGGCGGGCGCCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAA16890.11400007289525088No Hit
CTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGAACTGTGGCTGCACCA16680.11258266523935966No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16380.11055779715951507No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16160.10907289390096235No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16000.10799296425837858No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCC15970.10779047745039409No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG15950.10765548624507112No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15660.10569811376788803No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG15430.10414571490667383No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15180.10245832484013667No Hit
GTCTGAGACAGACTTCACCCTCAACATCAGCAGACTGGAGCCTGAAGATT14920.10070343917093802No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTCGGGCCAGTCAGA14920.10070343917093802No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)