FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481578
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTACACCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGAACTGTGGCTGCACCAT63940.43156688341754534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44610.30109788347289174No Hit
ATAGTACACCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGA42450.28651883329801064No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41880.2826715839463059No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36310.24507653326385787No Hit
CTATAATACTGATGACAGTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGT35720.24109429270683017No Hit
GTGTACTATAATACTGATGACAGTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGT32720.2208456119083842No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31360.211666209946422No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGCCTCAGTAGCAGGTGCCGT29040.19600723012895713No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27560.18601788093505708No Hit
CCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27340.18453297767650437No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25990.1754210713172037No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA25810.17420615046929694No Hit
GTCTCTGGTGCCGCCATCAGCAGTGGTACTTCCTACTGGGGCTGGTTCCG25680.1733287076346976No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25550.1724512648000983No Hit
ATAATAGACAGCCGTGTCAGCGACGGTCACAGAACTCAACCTCAGGGAGATCAAGTTCTGGGACGTGTCGGCCGA24310.1640818100700739No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23300.1572647542012638No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT22040.14876030826591646No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21920.14795036103397863No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21630.14599298855679554No Hit
CATCTGCACTGTCTCTGGTGCCGCCATCAGCAGTGGTACTTCCTACTGGG21440.1447105721062273No Hit
ATATATTACTGTCATCAGTATTATAGTACACCGTACACTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGAA21360.1441706072849354No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21110.1424832172183982No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20880.14093081835718402No Hit
ATATTACTGTCATCAGTATTATAGTACACCGTACACTTTTGGCCAGGGGA20470.13816349864806307No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20420.13782602063475566No Hit
ATTCAGGGGTGGGGACAGGCGGGCGCCTCAGTAGCAGGTGCCGTCCACCT20390.13762353382677117No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20310.1370835690054793No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20240.13661109978684888No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC20010.13505870092563468No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19930.1345187361043428No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19740.13323631965377455No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19170.1293890703020698No Hit
GTCTATTATTGTGCGAGACGCCGTTTGGTGGGAGCTATTCGACCCTTTGA18450.1245293869104428No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG18350.12385443088382793No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18330.12371943967850495No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18310.12358444847318197No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTCGGGCCAGTCAGACTATTAACAA18190.12277450124124412No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC17770.11993968592946169No Hit
ACTATAACCCGTCTCTCAAGAGTCGAGTCACCATGTCGGCCGACACGTCC17670.11926472990284682No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17610.11885975628687792No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17380.11730735742566373No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC17230.11629492338574143No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17150.11575495856444952No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17100.11541748055114211No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17070.11521499374315762No Hit
GTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGTCTGTCTCAGACCCACTGCCA17010.11481002012718872No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG16750.11305513445799006No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG16460.11109776198080694No Hit
GTCTGAGACAGACTTCACCCTCAACATCAGCAGACTGGAGCCTGAAGATT16410.11076028396749951No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGT16300.11001783233822317No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16200.1093428763116083No Hit
CTTTTGGCCAGGGGACCAGGCTGGACATCAAAGGAACTGTGGCTGCACCA16070.10846543347700897No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGTCGGGCCAGTCAGA15920.10745299943708668No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15830.10684553901313329No Hit
CAGTAATATATTGCACAATCTTCAGGCTCCAGTCTGCTGATGTTGAGGGTGAAGTCTGTCTCAGACCCACTGCCA15680.105833104973211No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCA15550.10495566213861167No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15050.10158088200553735No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC14960.10097342158158396No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14950.10090592597892248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)