FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138580
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCAC66290.5822164450455831No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48450.4255300461978956No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCCT40880.3590437211263153No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36490.3204869223067329No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT36140.31741291784503506No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCCTCTGAGTACCA33890.2976514605912628No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTC33050.29027384988318783No Hit
CCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCT32610.286409387131339No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT31050.272708110102057No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT30510.2679653603611516No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATG29800.26172952273885014No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26830.23564439916387078No Hit
GCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATTTTTTT26830.23564439916387078No Hit
GTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGC26270.23072599202515415No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC26020.22853027455251276No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25900.22747633016564495No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT24970.21930826116741908No Hit
GAGTACCACTGTAGTCTATTGAATAACAGAAGTAGCCAGCTTCATCCTCC24860.21834214547945688No Hit
CTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAAACGG22990.20191817878409948No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG22940.2014790352895712No Hit
GGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGG22580.19831720212896767No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22360.1963849707530433No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC22130.1943649106782132No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22050.19366228108696798No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG21990.19313530889353406No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21950.19278399409791142No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT21320.18725078606685522No Hit
GTGCTGGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAG20500.18004883275659153No Hit
CCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCC20380.1789948883697237No Hit
CCCCAGGACAAACGGCCAGGATCACCTGCTCTGGAGATGCGTTGTCAAAA20360.17881923097191238No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20300.17829225877847846No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20100.17653568480036536No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG20020.17583305520912013No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19710.17311036554304485No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19350.1699485323824413No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG19070.16748932881308295No Hit
CCACTGTAGTCTATTGAATAACAGAAGTAGCCAGCTTCATCCTCCACCTG18590.16327355126561155No Hit
CTACAGTGGTACTCAGAGGGTATTCGGCGGAGGGACCAAGTTGACCGTCC18490.162395264276555No Hit
GTGGTACTCAGAGGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA17800.15633508405206487No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17510.15378805178380087No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17390.15273410739693302No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT17340.15229496390240474No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17320.15211930650459343No Hit
GCTCTGGAGATGCGTTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAG17180.15088970471991428No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG16950.14886964464508423No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16620.14597129758119762No Hit
TCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGC16570.14553215408666936No Hit
GGATCACCTGCTCTGGAGATGCGTTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGGCCAGGCCC16300.1431607792162167No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGTTATGTCATGAGTTGGGTCCGCCAGGCTCCAGGGAGGGGG16000.14052591824904706No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCCT15810.13885717296983963No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15730.1381545433785944No Hit
GGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGAGCTGACACAGC15690.13780322858297178No Hit
GACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGGCTACTTCTGTTATT15570.1367492841961039No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCCTCTGAGTACCAC15500.13613448330376435No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15350.1348170528201795No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15180.1333239649387834No Hit
GCTCAGGGACAATGGCCACCTTGACTATCAGTGGGGCCCAGGTGGAGGAT15180.1333239649387834No Hit
GAAGAACACAGCCGTGTCCTCGACTCTCATGTTGTTCATTTGCAGATACA14930.13112824746614204No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC14630.1284933864989724No Hit
CTGATAGTCAAGGTGGCCATTGTCCCTGAGCTGGAGCCAGAGAATCTCTC14470.12708812731648192No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCCTCTGAGTAC14330.12585852553180277No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14270.12533155333836885No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14230.12498023854274623No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14200.12471675244602927No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC14200.12471675244602927No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14170.12445326634931231No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13970.12269669237119922No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13780.12102794709199177No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13710.1204131461996522No Hit
GGATGAAGCTGGCTACTTCTGTTATTCAATAGACTACAGTGGTACTCAGA13640.11979834530731262No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13600.11944703051169No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13330.11707565564123733No Hit
CTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCTCCTATGA13310.11689999824342602No Hit
AGTCAGGCCAGGCCCCTGTGCTGGTCATCTATGAGGACAACAAACGACCC13040.11452862337297336No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13020.11435296597516204No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC12880.1131233641904829No Hit
GTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC12860.11294770679267158No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12850.11285987809376591No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12790.112332905900332No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12770.11215724850252068No Hit
GGAGATGCGTTGTCAAAAAAATATGCTTATTGGTACCAGCAGAAGTCAGG12760.11206941980361504No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12620.11083981801893587No Hit
CCTTGACTATCAGTGGGGCCCAGGTGGAGGATGAAGCTGGCTACTTCTGT12600.11066416062112455No Hit
GCTTCATCCTCCACCTGGGCCCCACTGATAGTCAAGGTGGCCATTGTCCC12450.10934673013753973No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12440.10925890143863408No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG12420.10908324404082279No Hit
GCCTTAGCCCTGGATTCCAAGGCCTATCCACTTGGTGATCAGCACTGAGC12280.1078536422561436No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12260.10767798485833231No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12260.10767798485833231No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12180.10697535526708708No Hit
GGTCATCTATGAGGACAACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGGACAATGGC12020.10557009608459661No Hit
GACCAGCACAGGGGCCTGGCCTGACTTCTGCTGGTACCAATAAGCATATT11910.10460398039663442No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11800.1036378647086722No Hit
ACAAACGACCCTCCGGGATCCCTGAGAGATTCTCTGGCTCCAGCTCAGGG11800.1036378647086722No Hit
CCCTCGGTGTCAGTGTCCCCAGGACAAACGGCCAGGATCACCTGCTCTGG11720.10293523511742697No Hit
GTACCACTGTAGTCTATTGAATAACAGAAGTAGCCAGCTTCATCCTCCAC11680.10258392032180434No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG11650.1023204342250874No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11540.1013543185371252No Hit
GCTCTGGGAATCTCACCATGGCCTGGACCCCTCTCCTGCTCCCCCTCCTCACTTTCTGCACAGTCTCTGAGGCCT11480.10082734634369125No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11440.10047603154806864No Hit
CAGTGGTACTCAGAGGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT11430.10038820284916299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4300.082.85831570
GTCGTAT500.068.81917639
TCTAACT200.006434377753.537156
AATCGCG200.007595897751.3369136
ATGTCGT800.047.14471437
CCGTCGT305.895961E-446.63987447
GTGGTAT1750.046.406731
TATACAC6350.045.8221243
GGTATCA7700.045.7035981
TCGTATG5750.045.5700740
CGTATGC5700.045.46464541
TTATACA6450.045.113692
ATGCCGT5800.044.97327844
CGTAGAG6550.044.53643428
CTTATAC6600.044.092251
CTTGAAA5450.043.9793957
ATCTCCG6700.043.92955410
ACGTAGA6650.043.84161827
GACGTAG6700.043.49529626
CACGAGA6700.043.43415521
Produced by FastQC (version 0.11.5)