FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260922
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43520.3451442674487399No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC41530.32936216514582184No Hit
GGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCAAAGAGG40660.322462452078717No Hit
GTAATAGATGGCCGTGTCTGCGGCGGTCACATCATTCAGGCGCAGGTAAA36610.29034309814564263No Hit
GTCAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA31130.24688283652755683No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30730.24371055465762353No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29080.2306248919441488No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26690.21167050777129748No Hit
GACTTCACCCTCACCATTGCCGGCCTAGAGCCTGAAGATTTTGCAGTTTA26030.2064362426859076No Hit
GTCTGGGACAGACTTCACCCTCACCATTGCCGGCCTAGAGCCTGAAGATT25680.203660496049716No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT25060.19874345915131944No Hit
GCGTGACACCACGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT24930.1977124675435911No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT24630.19533325614114116No Hit
ATCTATTACTGTGCGAGAGCGGCCCTCCCAAATTGTTATAATGGTGAATGCTTCCCTGGCTATTACTATTACTAC24340.19303335178543954No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24080.19097136856998292No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24040.19065414038298958No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23670.1877197796533013No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23470.18613363871833466No Hit
CTCCTGCAGGGCCAGTCGAAGTGTAGGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT23300.18478541892361303No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23230.18423026959637473No Hit
GTGTCACGCTGCTGACAGAAATAAACTGCAAAATCTTCAGGCTCTAGGCCGGCAATGGTGAGGGTGAAGTCTGTC22980.18224759342766642No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22880.1814545229601831No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21950.17407896761258826No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21380.16955846594793333No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTTGGCCTCTT21200.16813093910646335No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21030.1667827193117417No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGAAGTGTAGGCATATACTTGGCCTGGTA20860.16543449951702008No Hit
GTATATGCCTACACTTCGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC20490.16250013878733183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20400.16178637536659682No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC20270.1607553837588685No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18910.1499696254010954No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC18560.14719387876490378No Hit
GTGTAGGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCC18320.14529050964294382No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17680.14021485865105057No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17270.13696326973436898No Hit
CAGTAATAGATGGCCGTGTCTGCGGCGGTCACATCATTCAGGCGCAGGTA17230.13664604154737564No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16720.1326013821632107No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16560.13133246941523744No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16380.12990494257376745No Hit
GGGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCA16270.12903256505953578No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA16040.12720850298432418No Hit
CTATTACTGTGCGAGAGCGGCCCTCCCAAATTGTTATAATGGTGAATGCTTCCCTGGCTATTACTATTACTAC15980.12673266070383418No Hit
CATCTATGATGCATCAAAGAGGCCAACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCAC15850.12570166909610586No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15840.12562236204935753No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC15540.12324315064690758No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCAAAGAGGCCAACTGGCATCCCAGCCAGATT15480.1227673083664176No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15450.12252938722617258No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15350.12173631675868926No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15240.12086393924445762No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15090.11967433354323266No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC15030.11919849126274266No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14720.11673997281354438No Hit
TACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCAAAGAGGCCAA14710.11666066576679604No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14340.11372630503710776No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14320.1135676909436111No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14260.11309184866312111No Hit
GCTCAGTCAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT14040.1113470936346578No Hit
CTGCTACTCTGGCTCCCAGAGACCACCGGAGATGTTGTCTTGACACAGTC13950.11063333021392283No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT13670.10841273290496953No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13600.10785758357773122No Hit
CACCATTGCCGGCCTAGAGCCTGAAGATTTTGCAGTTTATTTCTGTCAGCAGCGTGACACCACGTGGACGTTCGG13520.10722312720374456No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13470.10682659197000291No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13360.10595421445577125No Hit
GTCGAAGTGTAGGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG13290.10539906512853293No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13290.10539906512853293No Hit
CTACAACCCCTCCCTCGAGAGTCGAGCCACCATTTCATCAGACAAGTCTA13260.10516114398828794No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13170.10444738056755296No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13160.10436807352080461No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCGAAGTGTAGGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGG13150.10428876647405629No Hit
GTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCAAAGAGGCCAACTGGCATCCCAGC13090.1038129241935663No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12930.10254401144559298No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12890.10222678325859966No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12840.101830248024858No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12770.10127509869761966No Hit
GTTTATTTCTGTCAGCAGCGTGACACCACGTGGACGTTCGGCCAGGGGAC12750.10111648460412301No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)