FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260922
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45490.36076775565816127No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37850.30017717194243576No Hit
GGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCAAAGAGG35920.28487091192000774No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34430.2730541619545063No Hit
GTAATAGATGGCCGTGTCTGCGGCGGTCACATCATTCAGGCGCAGGTAAA32440.2572720596515883No Hit
GTGTCACGCTGCTGACAGAAATAAACTGCAAAATCTTCAGGCTCTAGGCCGGCAATGGTGAGGGTGAAGTCTGTC30920.24521738854584185No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30660.2431554053303852No Hit
GTCAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA28130.2230907225030573No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26040.20651554973265596No Hit
GACTTCACCCTCACCATTGCCGGCCTAGAGCCTGAAGATTTTGCAGTTTA25390.2013605916940144No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25220.20001237189929277No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24830.1969193970761078No Hit
GCGTGACACCACGTGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTT24520.19446087862690953No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24300.19271612359844623No Hit
GTCTGGGACAGACTTCACCCTCACCATTGCCGGCCTAGAGCCTGAAGATT24260.19239889541145289No Hit
ATCTATTACTGTGCGAGAGCGGCCCTCCCAAATTGTTATAATGGTGAATGCTTCCCTGGCTATTACTATTACTAC23800.18875077126102963No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCGAAGTGTAGGCATATACTTGGCCTGGTA22400.17764778471626316No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC21920.17384104647234327No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21790.17281005486461493No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21490.170430843462165No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21390.16963777299468166No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21370.169479158901185No Hit
CTCCTGCAGGGCCAGTCGAAGTGTAGGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCT21240.16844816729345669No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT21140.16765509682597338No Hit
GTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCAGTTGGCCTCTT20240.1605174626186235No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG20160.15988300624463686No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19870.15758310188893523No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19240.15258675794379034No Hit
GGGTGAAGTCTGTCCCAGACCCACTGCCACTGAATCTGGCTGGGATGCCA18950.15028685358808871No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18650.14790764218563876No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18320.14529050964294382No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18040.14306991233399052No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18030.1429906052872422No Hit
GTATATGCCTACACTTCGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC17640.13989763046405723No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC17430.1382321824823423No Hit
GTGTAGGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCC17260.13688396268762065No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17000.134821979472164No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC16860.13371168081768736No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC16510.13093593418149577No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC16510.13093593418149577No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16330.12950840734002578No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16290.12919117915303246No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16110.1277636523115625No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16040.12720850298432418No Hit
CAGTAATAGATGGCCGTGTCTGCGGCGGTCACATCATTCAGGCGCAGGTA15160.12022948287047097No Hit
CTATTACTGTGCGAGAGCGGCCCTCCCAAATTGTTATAATGGTGAATGCTTCCCTGGCTATTACTATTACTAC14680.11642274462655106No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14640.11610551643955773No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG14200.11261600638263111No Hit
CATCTATGATGCATCAAAGAGGCCAACTGGCATCCCAGCCAGATTCAGTGGCAGTGGGTCTGGGACAGACTTCAC14030.11126778658790948No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14010.11110917249441282No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13860.10991956679318785No Hit
ACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCAAAGAGGCCAACTGGCATCCCAGCCAGATT13810.10952303155944618No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13800.10944372451269786No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13420.10643005673626124No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13300.10547837217528126No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13280.10531975808178459No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC13100.10389223124031462No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13040.10341638895982465No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC13030.1033370819130763No Hit
GTTTATTTCTGTCAGCAGCGTGACACCACGTGGACGTTCGGCCAGGGGAC12990.10301985372608298No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12910.10238539735209631No Hit
GTCGAAGTGTAGGCATATACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG12780.10135440574436802No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12720.10087856346387802No Hit
TACTTGGCCTGGTACCAACAGAAGCCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCAAAGAGGCCAA12700.10071994937038135No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATA200.007662650751.2237727
GGTATCA12600.041.6815721
GTGGTAT3550.038.426031
GTATCAA29100.035.9781881
GATTCAC2000.035.8082581
TGGTATC3900.034.0895772
TGATGAA14600.031.34679670
GACGTTT450.004899628530.30424916
TATCAAC35600.029.4932292
ATCAACG35900.029.1448733
TAGCTCG500.00639350728.69814363
TCAACGC36750.028.47194
ATTCACT2650.028.300782
CAACGCA37750.027.716585
AACGCAG37550.027.4103896
TGACGTC500.00817019127.2922324
GACAGGT2300.026.6893841
ACGCAGA39100.026.4109557
CGCAGAG39000.026.3912858
CACTTTA2750.026.0258435
Produced by FastQC (version 0.11.5)