FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993041
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCAGATCTCAGGCTTCTCAGCTCCATGTAGGCTGTGTCTGTGGACGTGTCCTCGGT45890.46211586429966134No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33970.3420805384671932No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32780.3300971460392874No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCA29010.2921329532214682No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA28090.28286848176459983No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24350.24520639127689595No Hit
ATCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAATGGATGG23510.2367475260336683No Hit
GTGTATGGATAATTCAGTGAGGGTGTTTCCGGAAACCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC22300.2245627320523523No Hit
ACCTGGTACAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGAAACA21640.21791648078981632No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21380.21529826059548396No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21170.2131835442846771No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG20960.21106882797387017No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGGCT20520.20663799379884618No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19940.20079734874995092No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19270.19405039671070984No Hit
TCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAATGGATGGG19020.19153287729308258No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19010.19143217651637748No Hit
GTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCACCACCTACTTAGCCTGGTA18880.19012306641921128No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18720.18851185399192982No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18290.18418172059361096No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG18100.1822684058362142No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17760.17884457942824114No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC17640.17763617010778004No Hit
GTTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGAT17270.1739102413696917No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17190.17310463515605096No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16830.1694794071946677No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16620.1673646908838608No Hit
CTTCAGGCCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGTTCTGGGACAGACTTCACTCTCACCATCAGCAG16190.1630345574855419No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACGGG16120.16232965204860625No Hit
GTTTATTACTGTCAGCAGCGTAGCAACGGGCTCTCTTTCGGCGGAGGGAC16120.16232965204860625No Hit
GTGTATTACTGTGCAACAAATGGGCTCAGGGACTTACCCCGGCTCTACTA15990.16102054195144005No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15730.15840232175710772No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15420.1552805976792499No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15360.15467639301901936No Hit
GAGTGAAGTCTGTCCCAGAACCACTGCCACTGAACCTGGCTGGGATGCCA14780.1488357479701241No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14660.147627338649663No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14410.14510981923203575No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14390.14490841767862556No Hit
GCGTAGCAACGGGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT14360.1446063153485103No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14170.14269300059111356No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG14020.1411824889405372No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC14000.140981087387127No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAACCACTGCCA14000.140981087387127No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13910.1400747803967812No Hit
GTCAGAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAG13880.13977267806666593No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13790.1388663710763201No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13680.1377586625325641No Hit
GAATTATCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAATG13470.1356439462217572No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13460.1355432454450521No Hit
CAGTAATACACGGCCGTGTCTTCAGATCTCAGGCTTCTCAGCTCCATGTA13160.1325222221438994No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13030.13121311204673322No Hit
GCCTACATGGAGCTGAGAAGCCTGAGATCTGAAGACACGGCCGTGTATTACTGTGCAACAAATGGGCTCAGGGA12970.1306089073865027No Hit
GTCCCAGAACCACTGCCACTGAACCTGGCTGGGATGCCAGTGGGCCTGAA12730.1281920887455805No Hit
TTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC12610.1269836794251194No Hit
ACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAGGCACCCACGCCCAGGTCCACCTGGTA12550.12637947476488887No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12340.12426475845408196No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12320.12406335690067177No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG12270.12355985301714632No Hit
GAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGGCTCGTCATCTATGATGGATC12240.12325775068703106No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG12000.12084093204610888No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGC11970.1205388297159936No Hit
GTGAAAGACTCTACGGACAGAAGTTCCAGGGCAGAGTCATCATGACCGAG11960.12043812893928851No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTG11950.12033742816258342No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11890.11973322350235288No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT11720.11802131029836634No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11720.11802131029836634No Hit
GTGTATTACTGTGCAACAAATGGGCTCAGGGACTTACCCCGGCTCTACTACTACATGGACGTCTGGGGCAAAGGG11580.11661149942449507No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11290.11369117690004744No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG11060.11137505903583034No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11050.11127435825912527No Hit
CTGCTACTCTGGCTCCCAGATGGCACCGGAGAGATTGTGTTGACACAATT10830.10905894117161326No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10830.10905894117161326No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10830.10905894117161326No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10830.10905894117161326No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10790.10865613806479289No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10780.1085554372880878No Hit
GTCTTTCACCGGCCTCAGGATCAAAACCTCCCATCCATTCAAGCCCTTTT10620.10694422486080635No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10580.10654142175398598No Hit
GTATTACTGTGCAACAAATGGGCTCAGGGACTTACCCCGGCTCTACTACTACATGGACGTCTGGGGCAAAGGGAC10410.10482950854999945No Hit
CAGTGAAGGTCTCCTGCAAGGTTTCCGGAAACACCCTCACTGAATTATCC10370.10442670544317909No Hit
GGCCTGAAGGATCCATCATAGATGACGAGCCTGGGAGGCTGGCCAGGTTT10320.10392320155965364No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10260.10331899689942309No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTG10100.10170778447214164No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT10050.10120428058861618No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9970.10039867437497546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1850.054.15077241
AATCTCG2050.051.889836
TCGTATG2000.051.73983440
ATCTCGT2100.050.7162537
CTTATAC2300.050.211881
TCTCCGA2350.049.13364811
CGAGACA2400.048.1415423
CACGAGA2400.048.12456521
CCCACGA2350.047.69814719
ACGAGAC2450.047.1448122
ATGCCGT2150.046.80822844
GGTATCA9100.045.537991
TCCGAGC2550.045.28230713
GGCAATC2350.045.10035733
TTATACA2650.044.8573762
GTATGCC2250.044.5993542
AGGCAAT2650.043.8206832
ATCTCCG2650.043.5713510
CGTCTTC2500.043.32330348
GCAATCT2450.043.31661634
Produced by FastQC (version 0.11.5)