FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993041
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCTTCAGATCTCAGGCTTCTCAGCTCCATGTAGGCTGTGTCTGTGGACGTGTCCTCGGT49980.5033024819720434No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT34800.3504387029337157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28200.28397619030835586No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA26930.2711871916668093No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGGCA26440.2662528536082599No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25150.25326245341330317No Hit
GTGTATGGATAATTCAGTGAGGGTGTTTCCGGAAACCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC24230.24399798195643482No Hit
ATCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAATGGATGG22550.2270802514699796No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21610.21761437845970108No Hit
ACCTGGTACAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCGGAAACA21590.21741297690629088No Hit
GTCTTTGTCTCCAGGGCAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCACCACCTACTTAGCCTGGTA20820.2096590170999989No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20190.20331486816757816No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19910.20049524641983563No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19470.19606441224481164No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19090.1922377827300182No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18930.19062657030273675No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGGCT18730.18861255476863495No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18530.18659853923453312No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17980.18105999651575314No Hit
GTGTATTACTGTGCAACAAATGGGCTCAGGGACTTACCCCGGCTCTACTA17700.17824037476801058No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17630.17753546933107495No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16930.1704864149617186No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACGGG16810.16927800564125753No Hit
GTTTATTACTGTCAGCAGCGTAGCAACGGGCTCTCTTTCGGCGGAGGGAC16700.16817029709750153No Hit
TCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAATGGATGGG16390.1650485730196437No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16230.16343736059236225No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC16210.16323595903895205No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16140.16253105360201642No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG15970.16081914039802991No Hit
GTTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGAT15960.1607184396213248No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15350.15457569224231427No Hit
GAGTGAAGTCTGTCCCAGAACCACTGCCACTGAACCTGGCTGGGATGCCA15200.1530651805917379No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15170.15276307826162264No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA14790.14893644874682918No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG14520.14621752777579175No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14360.1446063153485103No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14230.14329720525134412No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14160.1425922998144085No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGAACCACTGCCA14130.1422901974842932No Hit
CTTCAGGCCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGTTCTGGGACAGACTTCACTCTCACCATCAGCAG13700.1379600640859743No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13670.137657961755859No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13540.13634885165869284No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13450.13544254466834701No Hit
GCGTAGCAACGGGCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT13330.13423413534788595No Hit
GAATTATCCATACACTGGGTGCGACAGGCTCCTGGAAAAGGGCTTGAATG13310.13403273379447575No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13290.13383133224106558No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13290.13383133224106558No Hit
TTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC13190.13282432447401468No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12960.1305082066097976No Hit
CAGTAATACACGGCCGTGTCTTCAGATCTCAGGCTTCTCAGCTCCATGTA12930.13020610427968232No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12860.1295011988427467No Hit
GCCTACATGGAGCTGAGAAGCCTGAGATCTGAAGACACGGCCGTGTATTACTGTGCAACAAATGGGCTCAGGGA12550.12637947476488887No Hit
GTCCCAGAACCACTGCCACTGAACCTGGCTGGGATGCCAGTGGGCCTGAA12460.12547316777454307No Hit
CCCCTACAGAAGCCCCCAGAGCGCAGCACCTCACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAG12410.12496966389101759No Hit
GTCAGAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAG12160.12245214447339033No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGAG11890.11973322350235288No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC11690.11771920796825107No Hit
GTCTTTCACCGGCCTCAGGATCAAAACCTCCCATCCATTCAAGCCCTTTT11690.11771920796825107No Hit
CTGCTACTCTGGCTCCCAGATGGCACCGGAGAGATTGTGTTGACACAATT11680.11761850719154597No Hit
GTGAAAGACTCTACGGACAGAAGTTCCAGGGCAGAGTCATCATGACCGAG11550.11630939709437978No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11300.11379187767675253No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11270.11348977534663726No Hit
ACCATGGACTGCACCTGGAGGATCCTCTTCTTGGTGGCAGCAGCTACAGGCACCCACGCCCAGGTCCACCTGGTA11130.11207996447276597No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG11080.11157646058924052No Hit
GAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGCCTCCCAGGCTCGTCATCTATGATGGATC11010.11087155515230489No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCGTGGTCCCTTTG10950.11026735049207434No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT10880.10956244505513871No Hit
GTATTACTGTGCAACAAATGGGCTCAGGGACTTACCCCGGCTCTACTACTACATGGACGTCTGGGGCAAAGGGAC10850.10926034272502344No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCACCACCTACTTAGCCTGGTACCAACAAAAACCTGGCCAGC10800.10875683884149798No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10790.10865613806479289No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG10760.10835403573467761No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10710.10785053185115216No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10630.10704492563751145No Hit
GTGTATTACTGTGCAACAAATGGGCTCAGGGACTTACCCCGGCTCTACTACTACATGGACGTCTGGGGCAAAGGG10550.10623931942387072No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10530.10603791787046053No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10460.1053330124335249No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC10430.10503091010340963No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10420.10493020932670453No Hit
GTCATGATGACTCTGCCCTGGAACTTCTGTCCGTAGAGTCTTTCACCGGC10340.1041246031130638No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG10310.10382250078294854No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA10190.10261409146248746No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9970.10039867437497546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACT150.002377459368.8382938
TACCGCT200.00762267451.2907633
GTGGTAT2300.047.231311
TGGTATC2400.046.6637152
GGTATCA8300.044.9906731
CGCGTGA350.001462641638.77761514
GTATCAA20500.036.1002771
TGTTAGA9400.035.46712570
AATGGTA2800.034.01948570
GTATACC400.002810007733.9475061
ACGTACC906.1045284E-933.93041213
GATTCAC1450.032.77691
ATACGTA951.0342774E-832.14460411
GTGCTAG851.3920544E-731.9505921
ATCAACG22950.031.6404113
CTCGTAT1951.29584805E-831.40260370
TATCAAC23050.031.3606662
TCAACGC23200.031.2994584
CAACGCA23400.031.031945
AAGGAGT703.0426872E-530.6090459
Produced by FastQC (version 0.11.5)