FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539364
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATATACAGCCGTGTCCTCAGCTGTCAGGCTGTTCATTTGCAAAAACA57660.37457027707546753No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACTTTCCAGGAACAGC48930.3178585441779852No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG45420.2950569196109562No Hit
TGGTAACACCAATCGGCCCTCAGGGGTCCCTGACCGAATCTCTGGTTCCAAGTCTGGCACCTCAGCCTCCCTGGC44100.2864819496883128No Hit
GTATATAACTGTGCGCGAGATGCGGACGTGACTGGTCGGAACTGGTACTT44080.28635202590160613TruSeq Adapter, Index 18 (95% over 21bp)
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39690.25783375471948156No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTC39170.25445573626510687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA38020.2469851185294706No Hit
GCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACTGGACCACCCAGGCTGGTGTCGTAGGA35290.2292505216440036No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33980.2207405136147136No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33820.2197011233210599No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG33240.21593333350656504No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT32870.2135297434524908No Hit
GGTTATGATGTACACTGGTACCAGCACTTTCCAGGAACAGCCCCCAAGCTCCTCATCCATGGTAACACCAATCGG32310.20989187742470267No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG31460.20437011648966716No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT30690.19936805070145852No Hit
GAATGTATACTATGTAGATTCCGTGAAGGGCCGATTCACCATCTCCAGGG29980.19475575627337005No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG29270.19014346184528158No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29240.18994857616522148No Hit
ACCCAGGCTGGTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAG28930.18793475747126734No Hit
GATGTACACTGGTACCAGCACTTTCCAGGAACAGCCCCCAAGCTCCTCAT28830.18728513853773376No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28450.18481658659030611No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27870.18104879677581132No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG27680.1798145208020975No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27540.17890505429515047No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT27080.17591680720089595No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26580.17266871253322802No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAG26530.17234390306646122No Hit
GATTATTACTGCCAGTCCTACGACACCAGCCTGGGTGGTCCAGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTA26110.16961550354562013No Hit
GTGTTACCATGGATGAGGAGCTTGGGGGCTGTTCCTGGAAAGTGCTGGTA25450.16532801858429844No Hit
GACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACTGGACCACCCAGG25210.16376893314381785No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACCAGAGATTCGGTC24980.16227480959669058No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT24010.15597350594141476No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC23890.15519396322117446No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC23640.15356991588734048No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23350.1516860209800931No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT22900.1487627357791919No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22560.14655403140517773No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA22400.14551464111152398No Hit
ACACTGGACCACCCAGGCTGGTGTCGTAGGACTGGCAGTAATAATCAGCC22240.14447525081787024No Hit
GTCCAGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC22120.1436957080976299No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAG22090.14350082241756987No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21840.1418767750837359No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC21170.13752432822906083No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20710.13453608113480633No Hit
CTCCTGTGCAGCGTCTGGATTCAGCTTCAGTCACTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG20250.13154783404055181No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTACGACACCAGCCTGGGTGGTCCAGTGTTCGGCGGAGGGACCAGG20010.1299887486000712No Hit
GCCTGGTCCCTCCGCCGAACACTGGACCACCCAGGCTGGTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCT19890.1292092058798309No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19660.12771508233270365No Hit
TTCCAGGAACAGCCCCCAAGCTCCTCATCCATGGTAACACCAATCGGCCC19640.12758515854599692No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG19620.1274552347592902No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAC19580.12719538718587675No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACA19510.12674065393240325No Hit
GCTCCAACATCGGGGCAGGTTATGATGTACACTGGTACCAGCACTTTCCA19430.12622095878557638No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC19350.1257012636387495No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19260.12511660659856927No Hit
GCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCC19240.12498668281186257No Hit
CAGTTATATACAGCCGTGTCCTCAGCTGTCAGGCTGTTCATTTGCAAAAA19170.12453194955838905No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19090.12401225441156216No Hit
AGCCTGGGTGGTCCAGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACG18810.12219332139766813No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGTGGCTCAGTAGCAGGTGCCGT18310.11894522673000019No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18010.11699636992939942No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC17920.1164117128892192No Hit
GGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAG17600.11433293230191169No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC17510.11374827526173147No Hit
GCCCTCAGGGGTCCCTGACCGAATCTCTGGTTCCAAGTCTGGCACCTCAG17180.11160453278107063No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17130.11127972331430383No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17100.11108483763424375No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17030.11063010438077023No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16900.10978559976717657No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAGCCTGGTC16870.10959071408711649No Hit
GCCTGGAGCCCAGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAACC16840.10939582840705643No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT16820.10926590462034969No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT16460.10692727645962878No Hit
ACACCAGCCTGGGTGGTCCAGTGTTCGGCGGAGGGACCAGGCTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCT16440.10679735267292206No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16050.10426383883214106No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT16040.10419887693878772No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG16040.10419887693878772No Hit
GCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC16020.10406895315208098No Hit
GTATACATTCCTTCCATCAGACGAAAAAGCTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTG15780.10250986771160038No Hit
ACGTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACTGGACCACCCAGGC15700.1019901725647735No Hit
CCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG15590.10127559173788656No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15570.10114566795117984No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTA15530.1008858203777664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)