FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588952
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT50560.8584740352354691No Hit
CTATTATACTGTTGACAGTAATAAGTAGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTC42690.7248468466021No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT21670.3679416998329236No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19860.33720914437848926No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTTTCAGGGAGA16190.2748950678493324No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13880.23567285619201564No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12100.20544968010975428No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11530.19577147203846834No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11520.1956016789144107No Hit
GTCTATTACTGTGCGAGAGCGGGGCCCCATTACGATCTTTCGACTGGCTATTTTAGGCACGGCTACTTTGACTA11250.19101726456485418No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11170.18965891957239298No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10810.18354636710631766No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10790.18320678085820238No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC10720.18201822898979883No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10380.1762452627718388No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9930.16860457218924463No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9740.1653785028321493No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG9270.15739822600143985No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACCT9200.1562096741330363No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8790.14924815604667274No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8620.14636167293769273No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8100.137532430486695No Hit
GTAGGAGAGAGAGTCACCATCACCTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAGA8090.13736263736263737No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT8060.13685325799046444No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7980.13549491299800323No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7940.13481574050177264No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7820.13277822301308087No Hit
GACCAGGGAAAGTCCCTAAATTGCTGATCTATGCTGCATCCAGTCTGCAA7740.13141987802061966No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7720.13108029177250438No Hit
GTCTGCATCTGTAGGAGAGAGAGTCACCATCACCTGTCGGGCGAGTCAGG7710.13091049864844675No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA7680.1304011192762738No Hit
GTCTCTGGTGGCTCCATAAGCAGTAGTTTCTGGTGGAGTTGGGTCCGCCA7570.12853339491163968No Hit
ACTTATTACTGTCAACAGTATAATAGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA7500.12734484304323612No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT7470.12683546367106316No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC7380.12530732555454435No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7330.12445835993425611No Hit
CAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACAT7260.12326980806585255No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7260.12326980806585255No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7000.11885518684035372No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6900.11715725559977724No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6780.11511973811108546No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG6670.11325201374645132No Hit
ATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA6640.11274263437427838No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6520.11070511688558661No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6460.10968635814124071No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA6400.10866759939689483No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6370.10815822002472188No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT6360.10798842690066424No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAGGTC6330.1074790475284913No Hit
GTAATAAGTAGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG6310.10713946128037598No Hit
GATTTCACTCTCACCATCAGCAGCCTCCAGCCTGAAGATTTTGCTACTTATTACTGTCAACAGTATAATAGTTAT6200.10527173691574185No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6090.10340401255110775No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6010.10204566755864655No Hit
GTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCC6010.10204566755864655No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG5990.10170608131053124No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATG200.00582538854.8961866
CGGTAAA200.00746140351.5662937
AAACCGT200.0077303451.106549
ATGCCGT751.6370905E-1146.284844
TACTGAA4350.043.0692370
TCGTATG758.6401997E-1041.4340840
CGTATGC856.366463E-1140.69518341
CCGTCTT953.6379788E-1240.35178847
GACAGTA9900.039.92160814
AATCTGT11000.038.8135568
TTATACT10200.038.7474444
TATTATA10350.038.5150762
TATACTG10450.038.472555
ATACTGT10800.038.172176
CTATTAT10300.038.046931
CGAGACA901.4006218E-1037.8824323
AGAGTGA10850.037.80716761
TACTGTT10850.037.361887
ATTATAC10500.037.3158873
TTCAGGC10600.037.27083236
Produced by FastQC (version 0.11.5)