FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences588952
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT50430.8562667246227196No Hit
CTATTATACTGTTGACAGTAATAAGTAGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTC40000.6791724962305926No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20290.3445102487129681No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15950.27082003287194883No Hit
GTAATAGACGGCCGTGTCCGCGGCGGTCACAGAACTCAGTTTCAGGGAGA14560.2472187886279357No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14240.24178540865809095No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12140.20612885260598485No Hit
GTCTATTACTGTGCGAGAGCGGGGCCCCATTACGATCTTTCGACTGGCTATTTTAGGCACGGCTACTTTGACTA11870.20154443825642834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11830.20086526576019778No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11700.19865795514744833No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11150.1893193333242777No Hit
AGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTC10760.18269740148602942No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10480.17794319401241526No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10470.1777734008883576No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9710.16486912345997637No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9370.1590961572420163No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9160.1555305016368057No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9090.15434194976840218No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8770.14890856979855743No Hit
GTCTCCATCCTCACTGTCTGCATCTGTAGGAGAGAGAGTCACCATCACCT8590.14585229356551976No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8520.14466374169711624No Hit
GTCTGCATCTGTAGGAGAGAGAGTCACCATCACCTGTCGGGCGAGTCAGG8500.14432415544900093No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8300.14092829296784798No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8300.14092829296784798No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7950.1349855336258303No Hit
GATCTATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCA7610.12921256740787024No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7480.1270052567951208No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7250.12310001494179493No Hit
ACTTATTACTGTCAACAGTATAATAGTTATCCCCGGACCTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA7230.12276042869367962No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7200.12225104932150667No Hit
GTAGGAGAGAGAGTCACCATCACCTGTCGGGCGAGTCAGGGCATTAGCAATTATTTAGCCTGGTATCAGCAGAGA7040.1195343593365843No Hit
GGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCT7020.11919477308846901No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7000.11885518684035372No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCGCTGAACTTTGATGGGACCCCA6960.11817601434412311No Hit
GTCTCTGGTGGCTCCATAAGCAGTAGTTTCTGGTGGAGTTGGGTCCGCCA6910.11732704872383487No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAGGTC6730.11427077249079721No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6720.11410097936673956No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6690.11359159999456661No Hit
GGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGTTTCC6620.11240304812616309No Hit
GCATGGACATGAGAGTCCTCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGT6580.11172387562993248No Hit
ATGCTGCATCCAGTCTGCAAAGTGGGGTCCCATCAAAGTTCAGCGGCAGTGGATCTGGGACAGATTTCACTCTCA6570.11155408250587484No Hit
GTAATAAGTAGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCG6470.10985615126529835No Hit
GACCAGGGAAAGTCCCTAAATTGCTGATCTATGCTGCATCCAGTCTGCAA6450.10951656501718306No Hit
GATTTCACTCTCACCATCAGCAGCCTCCAGCCTGAAGATTTTGCTACTTATTACTGTCAACAGTATAATAGTTAT6440.10934677189312543No Hit
GACCCAGTCAGGACACAGCATGGACATGAGAGTCCTCGCTCAGCTCCTGG6360.10798842690066424No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6320.10730925440443363No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG6170.10476235754356893No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6130.10408318504733832No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA6120.10391339192328067No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG6050.10272484005487714No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC5930.10068732256618534No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5930.10068732256618534No Hit
GCTCCTGGGGCTCCTGCTGCTCTGTTTCCCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCA5890.10000815006995477No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)