FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676650
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT23990.35454075223527676No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22350.3303037020616271No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACAATCTCCTG21380.3159683736052612No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG18460.2728146013448607No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC18010.26616419123623736No Hit
CTCCTGGACAGTCGATCACAATCTCCTGCACTGGAAGCAACAATGACATT17220.25448902682332075No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC16750.24754304293209192No Hit
GTGTATGAGTTGCAATAATAATCAGCCTCGTCGTCAGGCTGGAGCCCAGA16080.23764132121480824No Hit
GTAATAAAGGGCCGTGTCCGCGCCTGTCACACTGTCCATTTGAAGGTAGA15850.23424222271484518No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC15820.23379886204093697No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC15710.2321732062366068No Hit
CTCATACACAGGCAGCAATACCTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAA15430.22803517328013004No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC14850.21946353358457105No Hit
CTCCTGCACTGGAAGCAACAATGACATTGGTGTTTATGACTTTGTCTCCTGGTATCAACAACGTTCAGGCAAAGC14580.215473287519397No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT13010.19227074558486662No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12850.18990615532402275No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT12480.18443804034582134No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTG11850.1751274661937486No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11520.17025049878075815No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTGCTGC11430.16892041675903346No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG11380.16818148230251978No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11330.16744254784600607No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACAATCT11320.16729476095470333No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11070.1636000886721348No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10530.15561959654178675No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10260.15162935047661272No Hit
CTTTATTACTGTGCGAGGCGCAGCCAAAATTCTTTTGACATCTGGGGCCA10250.15148156358530998No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10220.15103820291140177No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10170.15029926845488806No Hit
CATTTATACCGATGGTCGGATATTCTACGCAGACTCCGTGAAGGGCCGAT10120.14956033399837435No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTGCTGCCTGTGTATGAGTTGCAATAATAAT10070.14882139954186063No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9570.14143205497672356No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9490.14024975984630164No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC9400.13891967782457695No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9280.13714623512894406No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9200.13596393999852213No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9180.13566836621591666No Hit
GTGTGACAGGCGCGGACACGGCCCTTTATTACTGTGCGAGGCGCAGCCAA8820.13034803812901796No Hit
GCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTGCTGCCTGTGTATGAGTTG8770.12960910367250425No Hit
GTCGATCACAATCTCCTGCACTGGAAGCAACAATGACATTGGTGTTTATG8740.12916574299859604No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA8730.1290179561072933No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8580.12680115273775217No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8540.1262100051725412No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA8430.12458434936821103No Hit
GTTTATGACTTTGTCTCCTGGTATCAACAACGTTCAGGCAAAGCCCCCACACGCATCATTTTTGATGTCACTAAT8420.12443656247690829No Hit
ACCTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC8340.12325426734648637No Hit
GACATAGGTATTGCTGCCTGTGTATGAGTTGCAATAATAATCAGCCTCGTCGTCAGGCTGGAGCCCAGAGATCGT8230.1216286115421562No Hit
GGTATCAACAACGTTCAGGCAAAGCCCCCACACGCATCATTTTTGATGTC8180.12088967708564251No Hit
GGCAAAGCCCCCACACGCATCATTTTTGATGTCACTAATCGACCCTCAGA8060.11911623439000961No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8000.11822951304219316No Hit
GCCCCCACACGCATCATTTTTGATGTCACTAATCGACCCTCAGAGGTTTCTGGTCGCTTCTCTGGCTCCAAGT7910.11689943102046849No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7820.11556934899874381No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7740.11438705386832189No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC7620.11261361117268899No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7590.11217025049878077No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7540.11143131604226707No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7470.11039680780314785No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT7430.10980566023793689No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7340.10847557821621222No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC7310.108032217542304No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7190.1062587748466711No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7170.1059632010640656No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG7090.10478090593364368No Hit
CCTCTGAGGGTCGATTAGTGACATCAAAAATGATGCGTGTGGGGGCTTTGCCTGAACGTTGTTGATACCAGGAGA7060.10433754525973546No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC7040.10404197147712997No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT6860.10138180743368064No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC6840.10108623365107515No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC6780.1001995123032587No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG6770.10005172541195596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAC150.002091716571.0944858
CGCATAA200.00685811852.6783551
CCGCATA200.006907898552.58210450
AATACGA200.00771892851.12646528
TCCGGTG200.00773701551.09620726
CGTATGC750.050.77200741
TCGTATG707.2759576E-1249.375340
CTACGCT1150.047.42164228
TCTCGTA800.047.3779338
ATCTCGT903.6379788E-1242.0413637
TACGCTG1300.041.9623429
ACTACGC1300.041.9437127
TTATACA1300.041.8910032
CGAGACT1300.041.8879123
ACGAGAC1300.041.8879122
CACGAGA1300.041.8848121
GAGACTA1400.038.91029424
CTTATAC1400.038.898791
TATACAC1500.038.574633
TGCATCG451.041431E-438.07719834
Produced by FastQC (version 0.11.5)