FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences676650
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACAATCTCCTG21580.31892411143131605No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC20780.3071011601270967No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT20660.3053277174314638No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA20020.29586935638808837No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC18730.27680484741003475No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG16910.2499076331929358No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC16140.2385280425626247No Hit
GTGTATGAGTTGCAATAATAATCAGCCTCGTCGTCAGGCTGGAGCCCAGA16130.23838025567132193No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC14910.2203502549323875No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14690.2170989433237272No Hit
CTCCTGGACAGTCGATCACAATCTCCTGCACTGGAAGCAACAATGACATT14420.21310869725855316No Hit
CTCATACACAGGCAGCAATACCTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAA14380.2125175496933422No Hit
CTCCTGCACTGGAAGCAACAATGACATTGGTGTTTATGACTTTGTCTCCTGGTATCAACAACGTTCAGGCAAAGC14360.21222197591073672No Hit
GTAATAAAGGGCCGTGTCCGCGCCTGTCACACTGTCCATTTGAAGGTAGA14140.2089706643020764No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT13770.20350254932387496No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC13330.19699992610655434No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG12220.18059558117195007No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12180.1800044336067391No Hit
GTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC12090.1786743515850144No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTG11460.1693637774329417No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT11140.16463459691125398No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACAATCT10860.1604965639547772No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT10620.1569496785635114No Hit
GACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTGCTGCCTGTGTATGAGTTGCAATAATAAT10510.15532402275918125No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10270.15177713736791548No Hit
ACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTGCTGC10140.14985590778097982No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG10140.14985590778097982No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG9940.146900169954925No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9890.1461612354984113No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA9800.1448311534766866No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG9720.14364885834626467No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9550.14113648119411806No Hit
CTTTATTACTGTGCGAGGCGCAGCCAAAATTCTTTTGACATCTGGGGCCA9380.13862410404197148No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9300.13744180891154956No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC9260.13685066134633858No Hit
GTGTGACAGGCGCGGACACGGCCCTTTATTACTGTGCGAGGCGCAGCCAA9160.13537279243331116No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8940.13212148082465086No Hit
GTCGATCACAATCTCCTGCACTGGAAGCAACAATGACATTGGTGTTTATG8900.13153033325943989No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA8810.13020025123771523No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8590.1269489396290549No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT8590.1269489396290549No Hit
CATTTATACCGATGGTCGGATATTCTACGCAGACTCCGTGAAGGGCCGAT8540.1262100051725412No Hit
GTTTATGACTTTGTCTCCTGGTATCAACAACGTTCAGGCAAAGCCCCCACACGCATCATTTTTGATGTCACTAAT8530.12606221828123845No Hit
GCCTTGGGCTGACGTAGGACGGTGACCCTGGTCCCAGTTCCGAAGACATAGGTATTGCTGCCTGTGTATGAGTTG8360.12354984112909184No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG8110.11985516884652331No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8100.11970738195522057No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC8090.11955959506391783No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT7810.11542156210744108No Hit
GACATAGGTATTGCTGCCTGTGTATGAGTTGCAATAATAATCAGCCTCGTCGTCAGGCTGGAGCCCAGAGATCGT7780.11497820143353285No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7680.11350033252050544No Hit
GGTATCAACAACGTTCAGGCAAAGCCCCCACACGCATCATTTTTGATGTC7500.11084016847705608No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC7450.11010123402054237No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7410.1095100864553314No Hit
ACCTATGTCTTCGGAACTGGGACCAGGGTCACCGTCCTACGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTC7410.1095100864553314No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC7380.10906672578142318No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG7380.10906672578142318No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA7240.10699770930318481No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC7150.10566762728146013No Hit
GTGTGAGGTAGAACTGGTGGAGACTGGAGGAGGCTTGATCCAGCCGGGGGGGTCCCTGAGACTCTCTTGTGAAGT7090.10478090593364368No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC7050.10418975836843272No Hit
GCCCCCACACGCATCATTTTTGATGTCACTAATCGACCCTCAGAGGTTTCTGGTCGCTTCTCTGGCTCCAAGT6920.10226852878149709No Hit
ACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACT6830.10093844675977241No Hit
GGCAAAGCCCCCACACGCATCATTTTTGATGTCACTAATCGACCCTCAGA6830.10093844675977241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT2100.040.4826621
TCTGGAG7100.036.01391270
ATCAACG18200.031.3641153
TCAACGC18450.030.9345534
AACGCAG18600.030.867736
CAACGCA18750.030.43965
GTAGTCA450.00491125830.28754628
GGCGTTG450.00498288330.1980179
TACCGCC450.00498288330.1980177
AATGGTA2951.8189894E-1229.65284770
CGCAGAG19550.029.3677648
GTGCTAT605.8975955E-428.3378621
CAGAGTA20750.027.99890510
AGAGTAC20900.027.96051611
GCAGAGT20550.027.9386779
TGTCCAT5200.027.62942933
ACACTGT5100.027.404629
GTGGCCG5900.027.37185970
TAGGTCA500.0082254627.25273127
GCTTACA500.00829707527.2043511
Produced by FastQC (version 0.11.5)