FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1116417
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51790.46389476333663854No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47490.42537868914572247No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA38900.3484361130294505No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC34770.3114427673530589No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT32230.28869141190075037No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31460.28179434745260956No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGAGCCAGTCAGAGTGTTATCAC30230.27077695878869634No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29330.2627154548882721No Hit
CTCCTGCAGAGCCAGTCAGAGTGTTATCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCT28940.2592221365314215No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28860.25850555840693934No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28450.25483309551896827No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27600.2472194529463453No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26220.23485848029902806No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24470.2191833338259808No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTTACGCC24450.21900418929486024No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTATT24270.21739188851477542No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24220.21694402718697406No Hit
GCGTTACGCCTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT23410.20968867367659216No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23380.20941995687991133No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22640.20279160922845135No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22580.20225417563508974No Hit
GTTTATTACTGTCAGCAGCGTTACGCCTGGCCTCCGATCACCTTCGGCCA22530.2018063143072884No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGAGCCAGTCAGAGTGTTATCACCTACTTAGCCTGGTA21280.19060978111225466No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21260.1904306365811341No Hit
CAATAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAG20880.18702689048984386No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTTACGCCTGG20860.18684774595872333No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC20100.18004025377614277No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGGCGTAACGCTG19790.17726351354377443No Hit
GTAATAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA19580.17538249596700875No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19490.1745763455769663No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19440.17412848424916497No Hit
TCTATGATGCATCCAATAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT18920.16947072644003092No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA18700.167500136597705No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18380.16463382409977634No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17780.15925948816616012No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC17670.15827419324499717No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT17440.15621403113711096No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17150.15361643543586312No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16700.14958568348565096No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16580.1485108162989277No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA16400.14689851551884286No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA16240.14546535926987855No Hit
CATCTATGATGCATCCAATAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16030.14358434169311288No Hit
GTGTTATCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC15880.14224075770970884No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15820.1417033241163472No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14900.13346267568480236No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14750.1321190917013983No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAGTTGTGTTGACACAGTC14540.13023807412463265No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14320.1282674842823067No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14190.1271030448300232No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14060.12593860537773968No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13940.12486373819101644No Hit
ACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATGCATCCAATAGGGCCACTGGCATCCCAGCCAGGTT13620.1219974256930878No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13540.12128084756860565No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13510.12101213077192483No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13480.12074341397524402No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13390.11993726358520157No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13340.11948940225740023No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGGCGTAACGCTGCTGA13270.11886239639847834No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13140.11769795694619484No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13110.11742924014951403No Hit
GCCCTATTGGATGCATCATAGATGAGGAGCCGGGGAGCCTGGCCAGGTTT13110.11742924014951403No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12880.11536907804162783No Hit
GTCTATTACTGTGTGAGAGGTGGTACCCATAAAGATGTGCTACATGACTA12870.11527950577606755No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12850.115100361244947No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12740.11411506632378404No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12680.11357763273042241No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA12640.11321934366818134No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT12370.11080089249805404No Hit
CCCAGATACCACCGGAGAAGTTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCAC12220.10945730851464998No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC12140.10874073039016782No Hit
GAGCCACCCTCTCCTGCAGAGCCAGTCAGAGTGTTATCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG11920.1067701405478419No Hit
GTCAGAGTGTTATCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATG11760.10533698429887757No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11750.10524741203331729No Hit
TTATTACTGTCAGCAGCGTTACGCCTGGCCTCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA11740.10515783976775704No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11700.10479955070551594No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG11670.10453083390883514No Hit
TCATAGATGAGGAGCCGGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGC11540.10336639445655163No Hit
GAGTGTTATCACCTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATCTATGATGCATC11510.10309767765987081No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11430.10238109953538865No Hit
ACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCCGGCTCCTCATC11420.1022915272698284No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11390.10202281047314758No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC11370.10184366594202703No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11330.10148537687978595No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC11240.10067922648974352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)