FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004688807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1138486
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAATATGGTACCTCACCGTCCATGTTCACTTTTGGCC32930.2892437851673187No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28000.2459406615452452No Hit
GTCTATGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA26030.22863697928652613No Hit
CAGTAATACACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25210.22143443134127255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25190.2212587594401688No Hit
GTTTATTACTGTGTGAGACATGGTTATTATTTTGATGTTAATGCTGTCTT21500.18884729368652756No Hit
GTAATAAACAGCCGTGTCTGCGGCTGTCACAGAGGTCAGCCTCAGGGAGA21310.18717841062604196No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18920.16618561844414423No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA17820.1565236638834382No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC17120.15037514734480706No Hit
GTGTATTACTGTCAGCAATATGGTACCTCACCGTCCATGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGA17010.14940895188873643No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16520.14510499031169466No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15620.13719975476202606No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15300.13438900434436613No Hit
CTCCTGCAGGGCCAGTCAGACTATAAGGAACAACCACTTAGCCTGGTACC14980.13157825392670616No Hit
CATCTATGGTGCATCCAGCAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA14890.1307877303717393No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG14710.1292066832618056No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14040.12332167457483009No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13980.12279465887151886No Hit
GTAATACACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13930.12235547911875948No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13910.12217980721765573No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGGCTGAAGATT13770.1209501039099295No Hit
ATATGGTACCTCACCGTCCATGTTCACTTTTGGCCAGGGGACCAAGCTGG13630.11972040060220328No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT12920.11348404811302028No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT12670.11128814934922346No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12610.1107611336459122No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12500.10979493818984158No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12490.10970710223928973No Hit
GTACCATATTGCTGACAGTAATACACTGCAAAATCTTCAGCCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC12420.10909225058542661No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12400.10891657868432286No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12290.10795038322825225No Hit
GTCTCCAGGCACCCTGTCTATGTCTCCGGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATAAGGAA12010.1054909766127998No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT11950.10496396090948856No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT11860.1041734373545217No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC11800.10364642165121046No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11790.10355858570065861No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT11740.10311940594789923No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC11720.10294373404679548No Hit
GTACCTCACCGTCCATGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAGACGAACTGTGGCTGCACCAT11590.10180186668962113No Hit
GATACAACCCGTCCCTCCAGAGTCGACTCACCATATCCATAGACACGTCCAACAATCGCTTCTCCCTGAGGCTGA11480.10083567123355053No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT11410.1002208195796874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)