FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716256
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA31360.43783228342938835No Hit
GTATTACTGTCAGCACTGTGGTCCCTTAAAGCGGTTTTTCGGCGGAGGGA30900.4314099986596971No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA21080.29430818031541794No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20730.28942165929500063No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20490.2860709020238574No Hit
ATATATTACTGTGCGAAAGATGTCTTTGATGGGCGAATACTACCTGCTGT17570.2453033552249475No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16440.22952687307331454No Hit
GGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTT16220.22645534557476657No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14920.2083054103560738No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14140.19741544922485815No Hit
GTGTATTACTGTCAGCACTGTGGTCCCTTAAAGCGGTTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13840.19322700263592904No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA13620.19015547513738107No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13490.18834048161551176No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12700.1773109055979985No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12630.17633360139391502No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12250.17102823571460485No Hit
CATCTATGGTGCATCCAGCCGGGCCACTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA12190.17019054639681902No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATA12170.16991131662422373No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12060.16837555287494974No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT11780.16446633605861594No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC11770.16432672117231828No Hit
CCTTTAGCACCTATGCCATGACGTGGGTCCGCCAGACTCCAGGGAAGGGG11610.1620928829915561No Hit
GTGTGAGGTGCAGCTGCTGGAATCTGGGGGAGGATTGGTACAGCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGC11460.15999865969709154No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11400.1591609703793057No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11000.15357637492740026No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA10970.15315753026850734No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10910.15231984095072154No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10770.15036523254255463No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10570.1475729348166019No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC10500.14659563061251843No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG10420.14547871152213734No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA10420.14547871152213734No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG10300.1438033328865657No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCA10250.14310525845507752No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10050.14031296072912477No Hit
ATATTACTGTGCGAAAGATGTCTTTGATGGGCGAATACTACCTGCTGTCGACTGGGGCCAGGGAACCCTGGTCAC9730.1358452843676004No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9700.1354264397087075No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9680.13514720993611223No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC9590.13389067595943352No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9550.13333221641424295No Hit
GAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9530.13305298664164766No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT9490.13249452709645715No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGGGTTCCCTGG9310.12998145914309966No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9230.12886454005271858No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCAGTGGCCCGGCT9190.12830608050752804No Hit
CCATAGATGAGGAGCCGGGGAGGCTGGCCAGGCTTCTGCTGGTACCAGGTTAATGTGCTGCTGCTAAGACTCTGA9180.1281664656212304No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9120.12732877630344458No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9070.1266307018719564No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG9010.12579301255417058No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCATCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG8920.12453647857749185No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8910.12439686369119421No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8900.12425724880489658No Hit
GGATTGGTACAGCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGCCTCTGG8810.12300071482821784No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8770.12244225528302731No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8740.12202341062413438No Hit
GCCATATATTACTGTGCGAAAGATGTCTTTGATGGGCGAATACTACCTGC8580.1197895724433722No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8560.11951034267077693No Hit
TAGTAACACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCA8440.11783496403520528No Hit
GATCAGGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGC8140.11364651744627619No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8130.11350690255997856No Hit
GCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGCCATGACGTGGGTCCG7980.11141267926551401No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTCTCAACTATTGGTGGTAGGGGTGAT7950.1109938346066211No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT7870.10987691551624No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA7860.10973730062994237No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCATCCTCTCCTGCAGGGCCAGTCAGA7830.10931845597104946No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGGGTTCCCTGG7710.10764307733547782No Hit
CTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACAATTCCAAGAACACTCTGTATCTCCAAATGAA7640.10666577313139436No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7590.10596769869990617No Hit
GGGCTGGAGTGGGTCTCAACTATTGGTGGTAGGGGTGATAGTAACACATA7520.10499039449582272No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAAAC7500.10471116472322746No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT7480.10443193495063219No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCATCCTCTCCTGC7420.10359424563284635No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7410.10345463074654872No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7350.10261694142876292No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTGCAGTGTGAGGTGCAGCTGCTGGAAT7330.10233771165616763No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7250.10122079256578652No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT7240.10108117767948889No Hit
CTCCAGCCCCTTCCCTGGAGTCTGGCGGACCCACGTCATGGCATAGGTGCTAAAGGTGAATCCAGAGGCTGCACA7230.10094156279319128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)