FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716256
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACTGTGGTCCCTTAAAGCGGTTTTTCGGCGGAGGGA29410.4106073806013492No Hit
GTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATACA27380.382265558682929No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT20320.28369744895679755No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19460.2716905687352008No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17560.24516374033864988No Hit
GGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17470.24390720636197116No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17360.24237144261269714No Hit
ATATATTACTGTGCGAAAGATGTCTTTGATGGGCGAATACTACCTGCTGT17030.23776415136487514No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14520.20272081490416835No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14410.20118505115489432No Hit
GTGTATTACTGTCAGCACTGTGGTCCCTTAAAGCGGTTTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13770.1922496984318456No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13720.1915516240003574No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13530.18889894116070233No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA12600.17591475673502213No Hit
GTGTGAGGTGCAGCTGCTGGAATCTGGGGGAGGATTGGTACAGCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGC12580.17563552696242685No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12490.1743789929857481No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11950.16683978912567574No Hit
CATCTATGGTGCATCCAGCCGGGCCACTGGCATCCCGGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA11520.16083634901487737No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11480.16027788946968682No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10990.15343676004110263No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA10940.15273868560961446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10710.1495275432247688No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCA10530.14701447527141134No Hit
CAGTAATATATGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGGAGATA10520.14687486038511371No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10410.14533909663583972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10370.14478063709064914No Hit
CCTTTAGCACCTATGCCATGACGTGGGTCCGCCAGACTCCAGGGAAGGGG10200.14240718402358932No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9990.13947527141133897No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9960.13905642675244603No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9780.13654335879908858No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC9470.13221529732386186No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9400.1312379931197784No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC9360.13067953357458784No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9260.1292833847116115No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG9240.1290041549390162No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT9170.12802685073493278No Hit
ATATTACTGTGCGAAAGATGTCTTTGATGGGCGAATACTACCTGCTGTCGACTGGGGCCAGGGAACCCTGGTCAC9110.12718916141714692No Hit
GAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT9020.1259326274404682No Hit
CCATAGATGAGGAGCCGGGGAGGCTGGCCAGGCTTCTGCTGGTACCAGGTTAATGTGCTGCTGCTAAGACTCTGA9000.12565339766787295No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8890.12411763391859892No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCCGGGATGCCAGTGGCCCGGCT8870.12383840414600367No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8810.12300071482821784No Hit
GGGCTGGAGTGGGTCTCAACTATTGGTGGTAGGGGTGATAGTAACACATA8790.12272148505562257No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG8560.11951034267077693No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG8560.11951034267077693No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC8450.11797457892150293No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGGGTTCCCTGG8420.11755573426261003No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8400.11727650449001475No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG8350.11657843005852657No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT8340.11643881517222891No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG8240.11504266630925256No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC8240.11504266630925256No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG8230.11490305142295493No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8230.11490305142295493No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8190.11434459187776437No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8120.11336728767368091No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGTCATCCTCTCCTGCAGGGCCAGTCAGAGTCTTAGCAG8090.112948443014788No Hit
GCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGCCTCTGGATTCACCTTTAGCACCTATGCCATGACGTGGGTCCG8080.11280882812849037No Hit
TAGTAACACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCA7890.11015614528883527No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC7840.10945807085734709No Hit
GGATTGGTACAGCCTGGGGGGTCCCTGAGACTCTCGTGTGCAGCCTCTGG7830.10931845597104946No Hit
CTCCAGGGAAGGGGCTGGAGTGGGTCTCAACTATTGGTGGTAGGGGTGAT7810.10903922619845419No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTGCAGTGTGAGGTGCAGCTGCTGGAAT7800.10889961131215656No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7790.1087599964258589No Hit
GTCTTTGTCTCCAGGGGAAAGAGTCATCCTCTCCTGCAGGGCCAGTCAGA7770.10848076665326363No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG7700.10750346244918019No Hit
GCCATATATTACTGTGCGAAAGATGTCTTTGATGGGCGAATACTACCTGC7680.10722423267658492No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGACCAGGGTTCCCTGG7680.10722423267658492No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC7610.10624692847250146No Hit
GATCAGGACTGAACAGAGAGAACCCACCATGGAGTTTGGGCTGAGCTGGC7410.10345463074654872No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7400.10331501586025109No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAAAC7330.10233771165616763No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA7220.10080194790689362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)