FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030654
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC53850.5224837821422126No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCAAAGACCACGAGGTTCATGTTGCCTGCATATGAG44180.42865986063218114No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCGCACCATCAGCGAGA31390.30456389826265656No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28840.27982232640634No Hit
GTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27770.26944056880388567No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGTTATCTGCCTTCCAG25400.2464454608433092No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC24470.2374220640486526No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCAAAGACCACGAGGTTCATG22720.22044255395118054No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTT22370.2170466519316861No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG21760.21112807984056725No Hit
GTGTATTACTGTGCGAGGCTGAAATTGACTGGTTATTACCCCAGGTATTACTTTGACTACTGGGGCCAGGGAACC21660.2101578221207117No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTT20870.20249278613385288No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAGCTCTAGTCTCC20790.2017165799579684No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGAATATAATTATGTCTCCTGGTACCAACAATATCCAGGCAAAGC19290.18716271416013522No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAG18400.17852742045342085No Hit
GTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCT17990.1745493638020131No Hit
GTCTATAATAGTGGGAGCACCAACTACAGTCCGTCCCTCAGGACTCGAGT17670.17144453909847535No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTTGGTGAATATA16950.1644586835155154No Hit
GTTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16910.16407058042757316No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAG16620.16125683303999208No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16600.16106278149602096No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16390.15902524028432433No Hit
GGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC15990.15514420940490212No Hit
GTCTGAGGATGAGGCTGACTATTTCTGCAGCTCATATGCAGGCAACATGAACCTCGTGGTCTTTGGCGGAGGGAC15750.1528155908772488No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15540.15077804966555217No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT15510.15048697234959552No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15470.15009886926165328No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15380.1492256373137833No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAG15060.14612081261024554No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG14980.1453446064343611No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGTTATCTGCCTTCCAGGCCACTGTCACGGCTC14950.14505352911840444No Hit
ATATAATTATGTCTCCTGGTACCAACAATATCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCAATAAGCG14930.14485947757443332No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACTCAGC14810.14369516831060666No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14650.1421427559588378No Hit
TCCTCAGACTGAAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCCGACCTGGAGCCAAAGAAGCGATCAGGGACG14610.1417546528708956No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14060.13641823541169004No Hit
ACATGAACCTCGTGGTCTTTGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCA13360.12962643137270122No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCGCACCATCAGCGA13140.12749186438901902No Hit
CCCCTCAGGCGTCCCTGATCGCTTCTTTGGCTCCAGGTCGGGCAACACGG13130.12739483861703346No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13130.12739483861703346No Hit
TCTCTGGGCTTCAGTCTGAGGATGAGGCTGACTATTTCTGCAGCTCATAT13100.12710376130107678No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACT12890.12506622008938015No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12810.1242900139134957No Hit
CCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG12710.12331975619364016No Hit
ACAATATCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCAATAAGCGCCCCTCAGGCGTCCCTGATCGCTT12610.12234949847378461No Hit
TCCTCACCCTCCTCACTCAGGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT12560.12186436961385684No Hit
CCTCAGGCGTCCCTGATCGCTTCTTTGGCTCCAGGTCGGGCAACACGGCCTCCCTGACCGTCTCTGGGCTTCAGT12490.12118518920995794No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12360.11992385417414572No Hit
CCCCTGGGAGCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12360.11992385417414572No Hit
GTATTACTGTGCGAGGCTGAAATTGACTGGTTATTACCCCAGGTATTACT12260.1189535964542902No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12230.11866251913833352No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11910.11555769443479577No Hit
CCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11800.11449041094295467No Hit
GTCAATAAGCGCCCCTCAGGCGTCCCTGATCGCTTCTTTGGCTCCAGGTCGGGCAACACGGCCTCCCTGACCGT11520.11177368932735914No Hit
GTTTGGGGGCTTTGCCTGGATATTGTTGGTACCAGGAGACATAATTATAT11470.11128856046743135No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11450.11109450892346025No Hit
TAATTATATTCACCAACGTCACCGCTGGTTCCAGTGCAGGAGATGGTGAC11430.11090045737948914No Hit
CCCCTCCCCTGGGAGCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG11390.11051235429154693No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAGCTCTAGT11350.1101242512036047No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGC11290.10954209657169137No Hit
GACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCT11210.10876589039580695No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAG10960.10634024609616807No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10950.10624322032418251No Hit
GCTCATATGCAGGCAACATGAACCTCGTGGTCTTTGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGG10840.1051759368323414No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10830.10507891106035586No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC10700.10381757602454364No Hit
GCTTGGTCCCTCCGCCAAAGACCACGAGGTTCATGTTGCCTGCATATGAGCTGCAGAAATAGTCAGCCTCATCCT10670.10352649870858698No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10630.10313839562064477No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC10500.10187706058483255No Hit
CCCTGACCGTCTCTGGGCTTCAGTCTGAGGATGAGGCTGACTATTTCTGCAGCTCATATGCAGGCAACATGAAC10440.1012949059529192No Hit
ATTATAGACAACTCCAATAAACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTACGAACTACTA10410.10100382863696256No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10410.10100382863696256No Hit
GCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA10350.10042167400504923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAA7150.060.69569870
ACTACGT200.00760325951.3239837
CGCGGAT200.00765066851.24334736
GGTATCA10050.047.7161751
CTAACGC452.1596552E-645.0170173
CGTATGC3900.044.28473741
TCGTATG3850.042.9482140
GCCGTCT3800.042.26307746
GTCTAGG405.6733486E-542.213691
CCGTCTT3950.040.74400747
ATGCCGT4200.040.5730244
CCTAACG504.4573044E-640.5153122
TATACAC4500.040.5153123
GTATGCC4550.040.34399442
TGCTTGA4500.039.70661555
ATCTCGT3400.039.24774637
CCACGGA7450.038.5937669
GTCGTAT901.2732926E-1038.18298339
ATCTCCG4600.038.1610410
CTCTTAT1350.037.523281
Produced by FastQC (version 0.11.5)