FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030654
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC51780.5023994473412028No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCAAAGACCACGAGGTTCATGTTGCCTGCATATGAG43130.4184721545736979No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38000.36869793354510827No Hit
GTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27950.2711870326996257No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCGCACCATCAGCGAGA27820.26992569766381347No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGTTATCTGCCTTCCAG27710.26885841417197237No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27310.26497738329255016No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAG22630.21956932200331053No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTC22590.21918121891536832No Hit
GTGTATTACTGTGCGAGGCTGAAATTGACTGGTTATTACCCCAGGTATTACTTTGACTACTGGGGCCAGGGAACC22270.21607639421183053No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTT21120.20491843043349173No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTT20370.19764149753457513No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAGCTCTAGTCTCC20340.19735042021861846No Hit
GACCAAGGACGGTCAGCTTGGTCCCTCCGCCAAAGACCACGAGGTTCATG19900.19308128625125406No Hit
CTCCTGCACTGGAACCAGCGGTGACGTTGGTGAATATAATTATGTCTCCTGGTACCAACAATATCCAGGCAAAGC19860.19269318316331185No Hit
TCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19260.18687163684417854No Hit
GGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC18640.18085603898107414No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAG18430.1788184977693775No Hit
GTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAGCTGTAGCTTCT18100.1756166472938542No Hit
GTCTGAGGATGAGGCTGACTATTTCTGCAGCTCATATGCAGGCAACATGAACCTCGTGGTCTTTGGCGGAGGGAC17120.16610812163926983No Hit
GTTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16580.16086872995204987No Hit
GTCTATAATAGTGGGAGCACCAACTACAGTCCGTCCCTCAGGACTCGAGT16470.15980144646020875No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15810.15339774550916216No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15550.15087507543753773No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCGGTGACGTTGGTGAATATA15430.14971076617371104No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15310.14854645690988438No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC15070.1462178383822311No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACTCAGC15060.14612081261024554No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG15010.14563568375031774No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGTTATCTGCCTTCCAGGCCACTGTCACGGCTC14980.1453446064343611No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14890.1444713744864911No Hit
ATATAATTATGTCTCCTGGTACCAACAATATCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCAATAAGCG14470.1403962920630978No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAG14440.14010521474714113No Hit
TCTCTGGGCTTCAGTCTGAGGATGAGGCTGACTATTTCTGCAGCTCATAT13110.12720078707306234No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACT12810.1242900139134957No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12750.12370785928158237No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12680.1230286788776835No Hit
TCCTCAGACTGAAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCCGACCTGGAGCCAAAGAAGCGATCAGGGACG12660.12283462733371237No Hit
GTTTGGGGGCTTTGCCTGGATATTGTTGGTACCAGGAGACATAATTATAT12320.11953575108620351No Hit
ACAATATCCAGGCAAAGCCCCCAAACTCATCATTTATGAGGTCAATAAGCGCCCCTCAGGCGTCCCTGATCGCTT12300.11934169954223241No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGCGCACCATCAGCGA12280.1191476479982613No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC12190.1182744160503913No Hit
CCCCTCAGGCGTCCCTGATCGCTTCTTTGGCTCCAGGTCGGGCAACACGG12150.11788631296244909No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11970.1161398490667091No Hit
GTATTACTGTGCGAGGCTGAAATTGACTGGTTATTACCCCAGGTATTACT11850.11497553980288244No Hit
CCCCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11810.11458743671494023No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11800.11449041094295467No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAGCCTGCAGCTCTAGT11640.11293799859118578No Hit
GGGCTGTTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11610.11264692127522913No Hit
TCCTCACCCTCCTCACTCAGGGCACAGCGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGT11550.1120647666433158No Hit
CCTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11520.11177368932735914No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11480.11138558623941691No Hit
CCTCAGGCGTCCCTGATCGCTTCTTTGGCTCCAGGTCGGGCAACACGGCCTCCCTGACCGTCTCTGGGCTTCAGT11470.11128856046743135No Hit
ACATGAACCTCGTGGTCTTTGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGGCTGCCCCCTCGGTCA11470.11128856046743135No Hit
GTCAATAAGCGCCCCTCAGGCGTCCCTGATCGCTTCTTTGGCTCCAGGTCGGGCAACACGGCCTCCCTGACCGT11450.11109450892346025No Hit
AGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCA11420.11080343160750358No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11400.11060938006353248No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11220.10886291616779249No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11100.10769860690396584No Hit
GCTCATATGCAGGCAACATGAACCTCGTGGTCTTTGGCGGAGGGACCAAGCTGACCGTCCTTGGTCAGCCCAAGG10890.10566106569226917No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCAAGGACGGTCAGC10860.1053699883763125No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC10850.10527296260432696No Hit
CCCCTCCCCTGGGAGCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG10790.10469080797241365No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCCTCATGCGTGACCTGGCAG10790.10469080797241365No Hit
GCTTGGTCCCTCCGCCAAAGACCACGAGGTTCATGTTGCCTGCATATGAGCTGCAGAAATAGTCAGCCTCATCCT10730.1041086533405003No Hit
CCCCTGGGAGCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC10650.10333244716461587No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10600.10284731830468809No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10500.10187706058483255No Hit
TAATTATATTCACCAACGTCACCGCTGGTTCCAGTGCAGGAGATGGTGAC10400.100906802864977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGAA6550.067.87672470
GCTACGC200.00685172452.69277249
GGTATCA11600.045.4114881
CCACGGA7100.043.73998369
TAGGTGA350.001502678538.56523514
GTATCAA27900.035.7040021
GAGTAAG400.00288424733.7675131
TGGTATC3100.032.6623842
AGGTATC751.9808376E-631.5010532
GTGGTAT3250.030.1310121
CATAGAT8750.030.0509458
GTATGTA450.00513397430.0155661
ATCAGCG8400.030.01146541
TAGATTT8600.029.9233860
ATCAACG33600.029.7273653
CAGCGAG8550.029.61877443
TCAACGC33800.029.4516284
TTTGTCC8750.029.41309764
GTCCACG9200.029.04187467
CAACGCA34450.028.8973395
Produced by FastQC (version 0.11.5)