FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031137
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCATACTGGTCCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT114131.1068364339559147No Hit
GTATGATACTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC96760.9383816117547911No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52540.5095346205208425No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51810.5024550568935069No Hit
ACATAAAGCAGGGCAGCCTCCTAAGTTGCTCATTTCCTCGGCATCTTCACGGAATTCCGGGGTCCCTGACCGATT50710.49178722129067237No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37910.3676524070031431No Hit
CTCCTAAGTTGCTCATTTCCTCGGCATCTTCACGGAATTCCGGGGTCCCT36490.3538812010431204No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33690.3267267104177234No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32810.3181924419354557No Hit
ACTTAGGAGGCTGCCCTGCTTTATGTTGGTACCAACCTAAGAAGTTCCTATTGATGGAGCTGGAAAAAACACT30240.29326849875428773No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29370.2848312105956822No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29220.28337650574075024No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28960.28085501732553486No Hit
ATACTGGTCCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA28330.2747452569348205No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27870.2702841620463624No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27870.2702841620463624No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27330.26504722456860724No Hit
GCTTTATGTTGGTACCAACCTAAGAAGTTCCTATTGATGGAGCTGGAAAA27300.2647562835976209No Hit
GTTTATTACTGTCAGCAGTATCATACTGGTCCGATCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA27210.2638834606846617No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26440.2564159757626775No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26000.25214884152154365No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC24800.24051120268208784No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24100.23372258002573856No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23240.2253822721907952No Hit
CTTCTTAGGTTGGTACCAACATAAAGCAGGGCAGCCTCCTAAGTTGCTCATTTCCTCGGCATCTTCACGGAATTC22760.22072721665501285No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCAT22530.2184966692107838No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22260.21587820047190626No Hit
GACCAGTATGATACTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT22020.2135506727040151No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGC21240.20598620745836876No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20620.19997342739131654No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAGTATCATACTGGT20120.19512441120820997No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTC19410.18823880822819858No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19120.18542637884199675No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19030.18455355592903758No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18460.17902567748029602No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18270.17718305133071552No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17660.17126725158732545No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC17510.1698125467323935No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17230.16709709766985378No Hit
GTACCAACATAAAGCAGGGCAGCCTCCTAAGTTGCTCATTTCCTCGGCATCTTCACGGAATTCCGGGGTCCCTGA17180.16661219605154312No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16700.16195714051576077No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA16580.16079337663181517No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC16240.15749604562730268No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16140.15652624239068136No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG16130.1564292620670192No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16000.15516851785941152No Hit
GTCAGGGACCCCGGAATTCCGTGAAGATGCCGAGGAAATGAGCAACTTAGGAGGCTGCCCTGCTTTATGTTGGTA15960.15478059656476298No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCATCAATAGGAACTT15440.1497376197343321No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15110.14653726905348174No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15040.1458584067878468No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15030.14576142646418466No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14850.1440157806382663No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14830.14382181999094204No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14740.14294899707798284No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14390.13955468574980823No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14360.13926374477882184No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14300.13868186283684902No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14230.1380030005712141No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT14210.13780903992388985No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14020.13596641377430932No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG13860.1344147285957152No Hit
GGATCTCTGGTGCCTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGG13830.13412378762472882No Hit
CCTAAGAAGTTCCTATTGATGGAGCTGGAAAAAACACTCTGGCTGGACTT13820.1340268073010667No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13800.13383284665374243No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13740.13325096471176961No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAGTATCATACTGGTCCGATCACTTT13700.1328630434171211No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT13700.1328630434171211No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13440.13034155500190567No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC13210.12811100755767663No Hit
GAATTCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATT13210.12811100755767663No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA13160.12762610593936596No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAGTATCATACTGGTCCGA13090.12694724367373103No Hit
GTCTGGGGCTGAGGCGAAGAAGCCTGGGGCCTCAGTCAAGGTTCCCTGCA12720.12335897169823214No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12490.1211284242540031No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC12460.12083748328301672No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12430.12054654231203031No Hit
CTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCATCAATAGGAACTTCT12350.11977069972273326No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12300.1192857981044226No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12260.11889787680977407No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG12150.1178310932494906No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTG11880.11521262451061304No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT11820.11463074256864024No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC11750.11395188030300532No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC11480.11133341156412775No Hit
TTATTACTGTCAGCAGTATCATACTGGTCCGATCACTTTCGGCGGAGGGA11300.10958776573820937No Hit
GCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATC11290.10949078541454725No Hit
GGTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGAT11160.10823004120693953No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG11020.10687231667566967No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11020.10687231667566967No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC10830.10502969052608917No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT10780.1045447889077785No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC10770.10444780858411637No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10750.1042538479367921No Hit
ACCATCAACTGCAAGTCCAGCCAGAGTGTTTTTTCCAGCTCCATCAATAG10750.1042538479367921No Hit
ATGTTGGTACCAACCTAAGAAGTTCCTATTGATGGAGCTGGAAAAAACAC10500.1018293398452388No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10440.10124745790326602No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAT200.00751004851.48440630
GTGGTAT2450.047.530161
GGTATCA12150.043.6929971
CCTGGTA3950.042.09631770
TGGTATC3000.039.95022
CACGAGT4400.034.88401470
GTATCAA26500.034.7666471
ATGCCGT1500.034.6378444
CGTATGC1500.034.5581541
CTCTTAT701.0305375E-634.2496721
TCGTATG1550.033.43028340
ATCTCGT1350.033.17429437
TATCTCG1350.033.1451836
AATCTGT23150.033.06365668
ACGAGAC1600.032.11374322
CTTATAC1600.032.109071
GAGCCTA1500.032.04876331
CGTATTC450.004199464331.27406557
ACGTATT450.004226162631.23359356
TACGTAT450.004258170331.18547855
Produced by FastQC (version 0.11.5)