FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1496832
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC47960.3204100393364118No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT45130.30150344193603557No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA42220.2820623824183342No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC40700.2719076021891568No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGCGCTCAGTTTCAGGGAGA38890.2598153967846759No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38650.25821201043270053No Hit
GATTATTACTGCTGCTCATATGCTGGCAGCTACCTTCGTTATGTCTTCGG37920.2533350436121088No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC36910.24658745938087911No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35440.23676671797502993No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34340.22941786386180946No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG33980.2270127843338464No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCACCTGCGCTGGAACCAG31630.21131295963742092No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30350.20276156576021892No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAACGAAGGTAGCTGCCAGCATATGAG30210.20182625705489993No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29800.19908713870360867No Hit
ACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAACGAAGGTAGC29700.19841906105695228No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT29690.19835225329228665No Hit
GACCTAGGACGGTGACCTGGGTCCCAGTTCCGAAGACATAACGAAGGTAG29170.19487824952967334No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG27740.18532473918248674No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27710.18512431588848982No Hit
CTCCTGGACAGTCAGTCACCATCACCTGCGCTGGAACCAGCAGTGATGTT27130.18124946553788268No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT26930.17991331024456986No Hit
TCTCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCTGCTCATAT26260.17543719001197194No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25920.17316572601334018No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC25280.16889002907473918No Hit
GTAATAATCAGCCTCATCGTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG25070.16748706601676075No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC23240.15526124508294853No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG22930.15319020437831368No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22520.15045108602702242No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG22340.14924854626304088No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTGGGTC22170.148112814263725No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG21920.14644262014708398No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21490.14356988626646142No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG21220.14176607662048915No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCACCTG20770.1387597272105353No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGG20690.13822526509321018No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20610.13769080297588507No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC20100.1342836069779374No Hit
GTGTATTACTGTGCGACATGGTTTCTGGATCCAAAAAGTGGAAACAACTA20060.13401637591927484No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC19890.13288064391995896No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19870.13274702839062769No Hit
GTCAGTCACCATCACCTGCGCTGGAACCAGCAGTGATGTTGGTGCTTATAATTATGTCTCCTGGTACCAACAATA19830.1324797973319651No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC19310.1290057935693518No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAG19170.12807048486403283No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18690.12486371216008209No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA18600.12426244227809133No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17820.11905143663417136No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17390.11617870275354883No Hit
CCCCTGAGGGCCGCTTAGTGACTTCATAAATCATGATCTTGGGGGCTTTGCCTGGGTATTGTTGGTACCAGGAGA16800.11223704463827604No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16750.11190300581494783No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16740.11183619805028219No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT16730.11176939028561655No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC16180.10809496322900633No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC16120.1076941166410125No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC16000.1068924234650248No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG15910.10629115358303404No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCACCTGCGCTGGAACCAGCAGTGATGTTGGTG15860.10595711475970583No Hit
ACAATACCCAGGCAAAGCCCCCAAGATCATGATTTATGAAGTCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTT15460.1032848041730802No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15320.10234949546776123No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15260.1019486488797674No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15240.10181503335043612No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15200.10154780229177357No Hit
CCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACCATCTCTGGGCTCCAGG15160.101280571233111No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAG15010.1002784547631264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAT10000.055.52012370
CTAACGC453.5826815E-852.5364463
GGTATCA13650.043.8190041
GTATCAA33450.037.581031
CCTAACG656.4299275E-736.379892
TAACGCC853.6579877E-935.7601054
CTATGGA10650.035.12405869
GTGGTAT4250.034.9853021
TACTTGG9350.034.0347770
TAGCGCG502.1096627E-433.773434
TGGTATC4350.033.3930362
TATCAAC39500.031.899842
ATCAACG40700.030.869083
TCAACGC41250.030.5393684
GCGCTCA10250.030.5350932
CAACGCA41400.030.5113145
AGCGCTC10150.030.46794531
AACGCAG41450.030.3125576
GCGACGC450.005123612530.0288517
ACGCCAT1052.8661816E-828.9505886
Produced by FastQC (version 0.11.5)