FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004688824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181635
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50560.42788170627985794No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC38600.3266660178481511No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30740.26014801525005604No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCAGCGTGACGCTGGTTGTATATGAGATG30490.25803230269922606No Hit
GTGTGGTGGTCTCCACTCCCGCCTGGACGGGGCTGCTATCTGCCTTCCAG28970.24516877039017973No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG28680.2427145438312169No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG28030.23721369119905894No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27440.23222060957910012No Hit
CTCCTGCACTGGCGCCAGCAGTGACGTTGGTGCTTATAATTATGTCTCCTGGTACCAACAACTCCCAGGCAAAGC27020.22866621249370578No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAAAACCAGCGTGACGCTGGTTGTATATGAGATGCAGTAATAAT24170.20454708941424382No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24150.2043778324101774No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG23670.20031566431258385No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC23330.19743829524345502No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22090.18694436099133827No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGCGCCAGCAGTGACGTT21970.18592881896693988No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTC21770.18423624892627588No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA21340.18059722333884828No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT21140.1789046532981843No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG20570.1740808286822919No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC20460.1731499151599267No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC20410.17272677264976072No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG20350.17221900163756151No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19770.16731054851963592No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGGTGATTATTACTGCATCTCATAT19450.16460243645457354No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19020.16096341086714594No Hit
GTCGATCACCATCTCCTGCACTGGCGCCAGCAGTGACGTTGGTGCTTATAATTATGTCTCCTGGTACCAACAACT18430.15597032924718715No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC18220.15419313070448995No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18080.15300833167602518No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17020.14403771046050598No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16470.13938314284868No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16370.13853685782834801No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTGGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16280.13777520131004922No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTGGA16170.136844287787684No Hit
GATTATTACTGCATCTCATATACAACCAGCGTCACGCTGGTTTTCGGCGGAGGGACCAGGCTGACCGTCCTAGGT16150.13667503078361762No Hit
TAGCAGCCCCGTCCAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG15750.1332898907022896No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC15680.1326974911880572No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15500.13117417815145963No Hit
GTCTCTGGTTACTCCATCAACAATGGTCGCTGCTGGGGCTGGGTCCGGCA15280.12931235110672923No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15060.12745052406199883No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT14940.12643498203760045No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC14790.12516555450710246No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14340.12135727191560845No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14330.12127264341357527No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14300.12101875790747565No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14200.12017247288714367No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG14130.11958007337291125No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13480.11407922074075329No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC13300.11255590770415569No Hit
GTAATAATCACCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG13300.11255590770415569No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA13200.11170962268382369No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC13100.1108633376634917No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12890.1090861391207945No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12500.1057856275414997No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT12180.1030775154764373No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12090.10231585895813852No Hit
GCCTGGTCCCTCCGCCGAAAACCAGCGTGACGCTGGTTGTATATGAGATGCAGTAATAATCACCCTCGTCCTCAG12000.1015542024398397No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT11920.1008771744235741No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT11860.10036940341137492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)