FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445391
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCCCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT157731.0912618108179724No Hit
GTATAATATTGCTGACAGTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGAC93240.6450849631691356No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT88760.6140898898637116No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC79330.5488480279730537No Hit
GACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAA69080.4779329607005994No Hit
CTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAG65990.45655466237163506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60350.4175340790139139No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCCCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA51990.3596950582921853No Hit
GTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC51640.35727356819019906No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT50540.34966317072681374No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG50200.3473108660563128No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC50080.3464806408784889No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC47250.32690116376814304No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG44350.30683738863739984No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43370.30005721635183835No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT43280.2994345474684705No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG41200.2850439777195236No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTG40710.28165389157674287No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG40190.2780562491395062No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG38860.26885458675195845No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT38140.26387323568501536No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37380.2586151428921309No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATCCGGGGTCCCTGA37000.2559860964956887No Hit
GTCTCCAGACTCCCTGGCTCTCTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGCCAGAGTCTTTTATA34690.2400042618225795No Hit
CTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGCCAGAGTCTTTTATACACCTCCCACAGTAAGAACTA34280.23716765913168134No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCAC32830.22713577156630974No Hit
GTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCA31930.2209090827326308No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA31560.21834922176767393No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT31390.21717306943242345No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31170.2156509899397464No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30540.2112923077561712No Hit
CAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGC29940.20714118186705188No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG29580.20465050633358034No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTA29580.20465050633358034No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28670.19835463206841608No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT27800.19233549952919313No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGCGGCAGTTTATTACTGTCAGCAATATTATACTACTCCCCTTACTTT26990.18673147957888211No Hit
GTATAAAAGACTCTGGCTGGACTGGCAATTGATGGTGGCCCTCTCGCCCAGAGAGAGAGCCAGGGAGTCTGGAGA26280.18181931394342432No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA26220.18140420135451238No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25800.17849841323212887No Hit
ATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAACAGCC25080.17351706216518575No Hit
GTCAGGGACCCCGGATTCCCGGGCAGAAGCCCAGTAAATGAGTAATTTAGGAGGCTGTCCTGGTCTCTGCTGGTA24980.17282520785033254No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24680.1707496449057729No Hit
CTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGCCAGAGTCTTTTA24520.16964267800200777No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT24010.1661142209962564No Hit
CCCTGGCTCTCTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGC23970.16583747927031509No Hit
GAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTAC23770.16445377064060865No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC23740.16424621434615272No Hit
ATACTACTCCCCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA23480.16244739312753434No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT23190.16044101561446003No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAAGAC22990.1590573069847536No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTT22930.15864219439584168No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT22810.15781196921801782No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGCGGCAGTTTATTACTGTCAGCAATATTATACTACT22780.15760441292356187No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22760.15746604206059123No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22620.15649744601979673No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22460.15539047911603157No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22040.1524846909936481No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21870.1513085386583976No Hit
GGATCTCTGGTGCCTTCGCGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTCTCTCTGGGCGAGAGGG21860.1512393532269123No Hit
TTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAGAC21730.1503399426176031No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21240.14694985647482237No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC21090.14591207500254255No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20860.14432081007838019No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20620.14266035972273247No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA20610.14259117429124715No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20590.1424528034282765No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG20430.14134583652451138No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20420.14127665109302603No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20330.14065398220965813No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20320.14058479677817282No Hit
CAATTACACCATGCACTGGGTCCGTCAGGCTCCGGGGAAGGGTCTGGAAT20270.14023886962074622No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20260.1401696841892609No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATC20060.13878597555955446No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19860.13740226692984805No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19840.13726389606687742No Hit
CTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATCCGGGGTCCCT19540.13518833312231776No Hit
CCATCAACAGCCTGCAGGCTGAAGATGCGGCAGTTTATTACTGTCAGCAA19480.13477322053340585No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19460.1346348496704352No Hit
GCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATCCGGGGTCCCTGACCGATT19290.13345869733518473No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19230.1330435847462728No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC19200.13283602845181683No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18840.13034535291834526No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18740.12965349860349207No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18620.1288232734256682No Hit
GCAAGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGATC18590.12861571713121225No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC18370.12709363763853518No Hit
CATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTCTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAG18230.12612504159774068No Hit
GTAATACACGGCGGTGTCCTCAGTTGTGAGACTGTTCATTTGCAGAAACA18080.12508726012546087No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17760.12287332631793059No Hit
CCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGG17690.12238902829753333No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17380.12024427992148837No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17250.11934486931217919No Hit
ATTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAAGACT17160.11872220042881129No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGAT17000.11761523352504617No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT16740.11581641230642781No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAA16710.11560885601197184No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCCGGTCTTCA16620.11498618712860395No Hit
AGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTTCGCGGACATCGTGATGA16190.11201121357473515No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15930.11021239235611678No Hit
GGCTGAAGATGCGGCAGTTTATTACTGTCAGCAATATTATACTACTCCCC15850.10965890890423421No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA15660.10834438570601311No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15530.10744497509670393No Hit
GGGTCAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGCGGC15090.10440081611134981No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT15050.10412407438540852No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14910.10315547834461403No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14900.1030862929131287No Hit
ACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATT14790.10232525316679017No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC14720.10184095514639291No Hit
CTCCCTGGCTCTCTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGCCAGAGTCTTTTATACACCTCCCA14660.10142584255748098No Hit
CAGTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCA14520.10045724651668649No Hit
GGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC14480.1001805047907452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT200.007408123551.66369639
TCGTATG1050.049.25695840
ATCGCGT306.446845E-445.8030637
GGTATCA12500.045.771651
TATCGCG306.480181E-445.75514636
CGTATGC1300.045.14917441
GACAGGT2300.041.4598271
ATCTCGT1600.040.7933537
CCGATCA2150.039.68135528
TCTCCGA2150.039.59622211
TATCTCG1650.039.5158136
AGACCGA2250.039.3893725
GTATCGC350.001389937739.1831735
ACCGATC2200.038.76202827
GTATCAA31950.038.373281
ATGGCAA2500.037.20195470
CTTATAC2300.037.01771
GTGGTAT3450.036.524131
CACGAGA2350.036.23510721
CCCACGA2450.036.1376119
Produced by FastQC (version 0.11.5)