FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1445391
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATACTACTCCCCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT149951.0374355451223924No Hit
GTATAATATTGCTGACAGTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGAC112230.7764680975597607No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT85940.594579598184851No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67590.46762433140928644No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60290.4171189664250019No Hit
GACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAA57710.3992691251017891No Hit
CTTTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAG55410.3833564758601652No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51050.3531916277325651No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC50010.34599634285809167No Hit
GTTTATTACTGTCAGCAATATTATACTACTCCCCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA49750.3441975216394733No Hit
GTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC49640.3434364818931348No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC48720.3370714221964852No Hit
GTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCA47750.33036043534240905No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46540.32198899813268517No Hit
GTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTG42320.2927927460458796No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT42030.2907863685328053No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA40450.27985507035812457No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38650.26740169269076675No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG37910.2622819707608529No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37180.2572314342624245No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36830.2548099441604383No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36680.2537721626881584No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34270.237098473700196No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA34190.2365449902483134No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33990.235161281618607No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG33670.23294734781107673No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33430.23128689745542902No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTA31340.21682714227499686No Hit
GTCTCCAGACTCCCTGGCTCTCTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGCCAGAGTCTTTTATA30380.21018534085240603No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30230.20914755938012622No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCAC30000.20755629445596385No Hit
GTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATCCGGGGTCCCTGA28720.19870055922584268No Hit
CTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGCCAGAGTCTTTTATACACCTCCCACAGTAAGAACTA28620.19800870491098946No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26980.1866622941473968No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG26760.18514021465471972No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA26510.1834105788675867No Hit
GTATAAAAGACTCTGGCTGGACTGGCAATTGATGGTGGCCCTCTCGCCCAGAGAGAGAGCCAGGGAGTCTGGAGA26370.1824419828267922No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG25830.17870596952658485No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25560.17683796287648118No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT25510.17649203571905456No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25350.17538506881528942No Hit
GTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCACTGAATCGGT25320.17517751252083347No Hit
CACCATCAACAGCCTGCAGGCTGAAGATGCGGCAGTTTATTACTGTCAGCAATATTATACTACTCCCCTTACTTT24680.1707496449057729No Hit
CAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGC24380.16867408196121328No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA24080.16659851901665362No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA23770.16445377064060865No Hit
ATACTACTCCCCTTACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA23170.1603026447514894No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA23040.15940323414218022No Hit
CTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAAGAC22830.15795034008098846No Hit
GTCAGGGACCCCGGATTCCCGGGCAGAAGCCCAGTAAATGAGTAATTTAGGAGGCTGTCCTGGTCTCTGCTGGTA22580.15622070429385543No Hit
ATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAACAGCC22510.1557364062734582No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22120.15303817444553067No Hit
GATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGCGGCAGTTTATTACTGTCAGCAATATTATACTACT22110.15296898901404535No Hit
CAGTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCA21480.1486103068304701No Hit
GAGTGAAATCTGACCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG21400.14805682337858753No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21360.14778008165264622No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA21300.1473649690637343No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21260.14708822733779303No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC21250.1470190419063077No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21110.14605044586551322No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20640.1427987305857031No Hit
TCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAATCTGACCCAGACCCGCTGCCACTGAATCGGTCAGGGACC20590.1424528034282765No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT20560.14224524713382053No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG20290.14037724048371686No Hit
CTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGCCAGAGTCTTTTA20120.1392010881484664No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19810.13705633977242143No Hit
GAGTAGTATAATATTGCTGACAGTAATAAACTGCCGCATCTTCAGCCTGCAGGCTGTTGATGGTGAGAGTGAAAT19570.13539588941677375No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTT19520.13504996225934712No Hit
GAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTAC19450.1345656642389499No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT19450.1345656642389499No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA19430.13442729337597922No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19190.1327668430203315No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC19190.1327668430203315No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT18960.13117557809616914No Hit
CCCTGGCTCTCTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAGC18650.12903082972012417No Hit
CTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATCCGGGGTCCCT18620.1288232734256682No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT18580.12854653169972693No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC18530.12820060454230034No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18510.12806223367932967No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC18290.1265401541866526No Hit
TTTATACACCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAGAC18070.12501807469397552No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18060.12494888926249022No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT17890.12377273692723975No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17830.12335762433832782No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17820.12328843890684252No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17800.12315006804387185No Hit
GGCTGAAGATGCGGCAGTTTATTACTGTCAGCAATATTATACTACTCCCC17550.12142043225673883No Hit
CAATTACACCATGCACTGGGTCCGTCAGGCTCCGGGGAAGGGTCTGGAAT17470.12086694880485627No Hit
CTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATC17320.11982916733257645No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17320.11982916733257645No Hit
CCATCAACAGCCTGCAGGCTGAAGATGCGGCAGTTTATTACTGTCAGCAA17210.1190681275862379No Hit
GGATCTCTGGTGCCTTCGCGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTCTCTCTGGGCGAGAGGG17190.11892975672326726No Hit
ATTTAGGAGGCTGTCCTGGTCTCTGCTGGTACCAAGCTAAGTAGTTCTTACTGTGGGAGGTGTATAAAAGACT17130.11851464413435535No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC17100.11830708783989938No Hit
GCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGGCTTCTGCCCGGGAATCCGGGGTCCCTGACCGATT16660.11526292885454524No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC16130.11159610098582322No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGAT16080.1112501738283966No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTAAGGGGAGTAGT16070.11118098839691128No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT16000.11069669037651404No Hit
GGGTCAGATTTCACTCTCACCATCAACAGCCTGCAGGCTGAAGATGCGGC15860.10972809433571955No Hit
GTAATACACGGCGGTGTCCTCAGTTGTGAGACTGTTCATTTGCAGAAACA15860.10972809433571955No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT15820.10945135260977826No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15520.10737578966521862No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGTCAGATTTCACTCTCACCATCAA15300.10585371017254154No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG15070.10426244524837916No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14900.1030862929131287No Hit
CCTTGGTCCCTCCGCCGAAAGTAAGGGGAGTAGTATAATATTGCTGACAGTAATAAACTGCCGCATCTTCAGC14880.10294792205015806No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG14870.10287873661867274No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG14840.10267118032421677No Hit
AGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGCCTTCGCGGACATCGTGATGA14780.10225606773530485No Hit
GCAAGATGGTGTTGCAGACCCCGGTCTTCATTTCTCTGTTGCTCTGGATC14660.10142584255748098No Hit
CATCGTGATGACCCAGTCTCCAGACTCCCTGGCTCTCTCTCTGGGCGAGAGGGCCACCATCAATTGCCAGTCCAG14570.1008031736741131No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG14570.1008031736741131No Hit
CCTCCCACAGTAAGAACTACTTAGCTTGGTACCAGCAGAGACCAGGACAGCCTCCTAAATTACTCATTTACTGGG14500.10031887565371583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT451.614834E-460.6249770
TAATGCG200.00621283754.0142164
GGTATCA13800.045.3648341
GGTACGT508.905797E-539.1057868
GTGGTAT3600.037.804031
GTATCAA33750.036.3926771
ATTCACT4200.034.8204542
AATGGTA3550.034.5818570
TGGTATC4250.032.81032
TATCAAC38350.031.9265792
GACAGGT2350.031.8519081
GTACGTA550.003946954431.67316869
ATCAACG39800.030.6694813
CGACTCC4600.030.60452768
TCAACGC40400.030.2992044
AACGCAG40200.030.1961966
TCACTTT4850.030.1464944
CAACGCA40700.030.0758695
ACGACTC4600.029.96430467
ATAGCGG604.2794537E-429.93659863
Produced by FastQC (version 0.11.5)