FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687946
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAGCATCTCCTG24080.35002747308655036No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23620.34334090175682397No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23570.3426141005253319No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21240.3087451631378044No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGTTTGGTCCCTC19230.27952775363182575No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT17870.2597587601352431No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17740.25786907693336397No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16460.2392629654071686No Hit
GCCTTGGGCTGACGAAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACAGTGGTGCTGCTTCCATATGAACTG15740.22879702767368368No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15620.22705270471810288No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14380.2090280341771011No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14350.20859195343820588No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14120.20524866777334266No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAG14080.20466722678814908No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13580.197399214473229No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13460.19565489151764817No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGATAATTTTGATTATGTCTCCTGGTATCAACAACACCCAGGCAAAGC13430.195218810778753No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA12590.18300855008968728No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAGCATCT12510.18184566811930006No Hit
CTCCTGGACAGGCGATCAGCATCTCCTGCACTGGAACCAGCAGTGACGTT12430.18068278614891284No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12330.17922918368592883No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC12140.17646733900625922No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12090.1757405377747672No Hit
GCAATAATAGTCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATGCTCAGGG11820.17181581112471037No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11640.16919932669133914No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG11580.16832716521354874No Hit
CTATTATTGCAGTTCATATGGAAGCAGCACCACTGTGGTCTTCGGCGGAG11470.16672820250426632No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG11450.16643748201166952No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10920.15873338895785427No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10910.15858802871155586No Hit
GTTTGGTCCCTCCGCCGAAGACCACAGTGGTGCTGCTTCCATATGAACTGCAATAATAGTCAGCCTCGTCCTCAG10790.15684370575597503No Hit
CCACTGTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTTCGTCAGCCC10010.14550560654469974No Hit
GGCCTGGGCTCTGCTGCTCCTAACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC9970.14492416555950613No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9850.1431798426039253No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9710.14114479915574768No Hit
GAGCATCTCTGCGCTCCAGGCTGAGGACGAGGCTGACTATTATTGCAGTTCATATGGAAGCAGCACCACTGTGGT9550.13881903521497327No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9320.13547574955011002No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9190.13358606634823084No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9180.13344070610193243No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC8970.130388140929666No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGT8960.1302427806833676No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAG8890.12922525895927878No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTCACTTATCGGCCCTCAGGGGTTTCTGATCG8820.12820773723518997No Hit
CCACAGTGGTGCTGCTTCCATATGAACTGCAATAATAGTCAGCCTCGTCC8790.12777165649629477No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG8710.12660877452590755No Hit
GGACCAAACTGACCGTCCTTCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8410.12224796713695553No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCG8360.12152116590546352No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC8330.12108508516656831No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8320.12093972492026991No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8070.11730571876280987No Hit
CAGTGGTGCTGCTTCCATATGAACTGCAATAATAGTCAGCCTCGTCCTCA8050.11701499827021307No Hit
CTTATCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGAGCATCTCTG7860.11425315359054344No Hit
GGTATCAACAACACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTC7700.11192738964976902No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC7610.1106191474330834No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACAGTGGTG7580.1101830666941882No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7580.1101830666941882No Hit
CTCCTGATTTATGAGGTCACTTATCGGCCCTCAGGGGTTTCTGATCGCTT7540.1096016257089946No Hit
TCTCTGCGCTCCAGGCTGAGGACGAGGCTGACTATTATTGCAGTTCATAT7460.1084387437386074No Hit
GACGAAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACAGTGGTGCTGCTTCCATATGAACTGCAATAATAGT7460.1084387437386074No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7380.10727586176822018No Hit
CCCCTGAGGGCCGATAAGTGACCTCATAAATCAGGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGATACCAGGAGA7280.10582225930523617No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAGCATCTCCTGCA7240.10524081832004256No Hit
TCCTTCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG7220.10495009782744576No Hit
CTGCTGCTCCTAACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC7140.10378721585705855No Hit
GCACCACTGTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTTCGTCAGCCCAAGGCTGCCCCCTCGGTCACT7050.10247897364037294No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG7040.10233361339407454No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC6890.10015320969959851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2900.056.59012270
GCCGTCT3650.044.9624446
CGTATGC3550.044.7206541
TCGTATG3550.044.63437740
CGAGACA3800.044.42069223
TATACAC4000.043.874883
ATCTCCG3950.042.7245110
ACGAGAC3950.042.7245122
ATGCCGT3750.042.6398744
TACACAT4050.042.499895
CACGAGA3900.042.40373221
CCCACGA4000.042.1843219
GTATGCC3900.041.68827442
CCGTCTT3900.041.26560647
TCTCCGA4100.041.16142311
ATACACA4300.040.8138434
CTTATAC4150.040.6714131
CTTGAAA4150.039.8581157
GCCCACG4250.039.70000518
TTATACA4300.039.244082
Produced by FastQC (version 0.11.5)