FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences687946
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38400.558183345785861No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC22980.33403784599372627No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22210.32284510702874936No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21780.3165946164379181No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAGCATCTCCTG21510.31266988978786125No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19040.27676590895215614No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGTTTGGTCCCTC16900.24565881624429822No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16220.23577431949600697No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC16040.23315783506263574No Hit
GCCTTGGGCTGACGAAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACAGTGGTGCTGCTTCCATATGAACTG15890.23097743136815974No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15100.21949397191058598No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15090.2193486116642876No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAG14970.2176042887087068No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14350.20859195343820588No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG13740.1997249784140034No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGATAATTTTGATTATGTCTCCTGGTATCAACAACACCCAGGCAAAGC13330.19376520831576896No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11980.17414157506548478No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC11980.17414157506548478No Hit
GTTTGGTCCCTCCGCCGAAGACCACAGTGGTGCTGCTTCCATATGAACTGCAATAATAGTCAGCCTCGTCCTCAG11860.17239725210990398No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA11640.16919932669133914No Hit
CTCCTGGACAGGCGATCAGCATCTCCTGCACTGGAACCAGCAGTGACGTT11590.16847252545984714No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG11320.16454779880979029No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11250.16353027708570148No Hit
GCAATAATAGTCAGCCTCGTCCTCAGCCTGGAGCGCAGAGATGCTCAGGG11020.16018699142083828No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC10760.15640762501707983No Hit
GGCCTGGGCTCTGCTGCTCCTAACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC10760.15640762501707983No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG10720.15582618403188622No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG10530.15306433935221658No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10290.14957569344105495No Hit
CTATTATTGCAGTTCATATGGAAGCAGCACCACTGTGGTCTTCGGCGGAG10210.14841281147066773No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAGCATCT10200.14826745122436935No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10070.14637776802249014No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC10000.14536024629840133No Hit
GAGCATCTCTGCGCTCCAGGCTGAGGACGAGGCTGACTATTATTGCAGTTCATATGGAAGCAGCACCACTGTGGT9690.1408540786631509No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9240.13431286757972283No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9230.13416750733342445No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA9090.13213246388524683No Hit
CCACTGTGGTCTTCGGCGGAGGGACCAAACTGACCGTCCTTCGTCAGCCC9070.13184174339265No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9060.1316963831463516No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAG9000.1308242216685612No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8980.1305335011759644No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGT8840.12849845772778679No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC8590.12486445157032673No Hit
CCACAGTGGTGCTGCTTCCATATGAACTGCAATAATAGTCAGCCTCGTCC8530.12399229009253633No Hit
CAACAACACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTCACTTATCGGCCCTCAGGGGTTTCTGATCG8430.1225386876295523No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT8350.12137580565916511No Hit
GGACCAAACTGACCGTCCTTCGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG8340.12123044541286672No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGAAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACAGTGGTG8200.1191954019646891No Hit
CTCCTGATTTATGAGGTCACTTATCGGCCCTCAGGGGTTTCTGATCGCTT7980.11599747654612426No Hit
CTGCTGCTCCTAACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC7920.11512531506833384No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC7890.11468923432943864No Hit
GGTATCAACAACACCCAGGCAAAGCCCCCAAACTCCTGATTTATGAGGTC7880.11454387408314025No Hit
GCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCGATCAGCATCTCCTGCA7760.11279955112755942No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG7720.11221811014236582No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC7350.10683978102932498No Hit
CAGTGGTGCTGCTTCCATATGAACTGCAATAATAGTCAGCCTCGTCCTCA7170.10422329659595375No Hit
GGGCTCTGCTGCTCCTAACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT7160.10407793634965536No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT7110.10335113511816335No Hit
GACGAAGGACGGTCAGTTTGGTCCCTCCGCCGAAGACCACAGTGGTGCTGCTTCCATATGAACTGCAATAATAGT7080.10291505437926814No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGGCG7040.10233361339407454No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG7000.10175217240888093No Hit
CCCCTGAGGGCCGATAAGTGACCTCATAAATCAGGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGATACCAGGAGA6890.10015320969959851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATATG608.792973E-636.69392464
GTGGTAT2700.035.0150381
GATACAC450.003891571431.76271655
CTAGATA450.0043970330.98010345
GATCGCA500.0059103529.16140762
AGACCGG500.00672021128.40486752
TCAACGC20900.027.1132164
ATATAGC500.00863580926.9821476
AACGCAG21200.026.886696
CAACGCA21200.026.7275985
TCGCACG550.00911192126.68649364
ATCGCAC550.00927470726.58975863
CGATCGC550.009563277526.4231861
ACGCAGA22350.025.503267
TACGACC700.00116530225.21867250
AGAGTAC23600.024.57761811
GCAGAGT23900.024.4102129
CAGAGTA24050.023.97752810
ATACGTA851.683565E-423.80431611
TACGTAC851.683565E-423.80431612
Produced by FastQC (version 0.11.5)