FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377891
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT7870.20826111233133363No Hit
ATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCT4330.11458330576806539No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC3870.10241048344628478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1200.046.68982346
CTAACGC606.9921953E-944.8538783
TCGTATG1250.044.26103640
CCTAACG551.7877574E-742.8150632
CTCGTAT1050.042.67842539
ACGAGAC1350.042.36760322
GGTATCA7200.041.5887871
CGTATGC1350.041.07747341
TCGATCC602.8517752E-740.3420940
TTCGATC602.9225885E-740.2162139
GAGACCG1350.039.9229724
CCGTCTT1500.039.7850447
GTATGCC1400.039.70248842
TATACAC1500.038.1257973
TCTCGTA1200.037.23753438
GTATCAA15250.036.1820141
CACGAGA1500.035.88310221
AGACCGA1600.033.69840625
CGGTCCA400.002943344433.622610
GTCCAAC400.002943344433.62268
Produced by FastQC (version 0.11.5)