FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004688832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377891
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16510.43689847072303917No Hit
GTATCAACGCAGAGTACGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCC3950.10452749602398576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGT150.002628304867.110496
TCTATCG252.8005432E-454.2265731
TAGCGAT200.00820852950.33286713
TACGACG307.2304806E-444.7403265
CGGTAGA405.128968E-542.9325838
GTCATAT504.6381065E-640.2716261
ACTCGCT350.00143054938.94668634
GTATCAA16400.036.2199061
GGTATCA7400.034.01321
CCGAAAC1001.5183468E-731.62300968
ATCAACG20000.029.5325243
AACGCAG20050.029.4549796
TCAACGC20500.028.8122224
CAACGCA20600.028.6685625
AGAGTAC20700.028.53006711
ACGCAGA21000.028.2822787
CGCAGAG21000.028.1224928
CCGAGTT656.371059E-427.96147259
CCATAAG550.007554483627.72810667
ACGGGAC855.460357E-627.64836516
Produced by FastQC (version 0.11.5)