FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004688833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004688833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789383
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT36220.45883937201586555No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34390.435656709100652No Hit
GAGTTACAGTACCCCTTGGACGTTCGGCCAGGGGACCAAGGTAGAAATCAAACGAACTGTGGCTGCACCAT29920.3790302046028354No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25440.32227701888690286No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21980.27844531741879414No Hit
GTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCT20320.2574162352115513No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20310.2572895539934354No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG20250.25652946668473986No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19690.24943531847024827No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19650.2489285935977846No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCACCTATTTAAATTGGTATCGGCAGAGA19390.2456348819267707No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17770.22511252459199146No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT17740.22473248093764372No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17140.21713160785068847No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAC17020.21561143323329737No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTC16930.21447130227025413No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG16780.2125710839985153No Hit
GATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA16460.20851728501880581No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15830.2005363682775028No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15520.19660925051590925No Hit
GTAATACACGGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATACA14960.1895151023014177No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14710.18634807184851968No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACTTCCAGATGACCCAGTC14640.18546130332170824No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14370.18204091043257836No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCCTGGCCGAACGTCCAAGGGGTA14240.1803940545970714No Hit
GACCAGGGAAAGCCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAA13750.17418667490939127No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13500.17101964445649323No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13410.16987951349344996No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTTGGACGTTCGGCCAGGGGACCAAGGTAGAAATCAAACGAA13040.16519230842316088No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACTT12790.16202527797026287No Hit
GATTTCACTCTCACCATCAGTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC12610.1597450160441763No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12430.1574647541180897No Hit
CCATCAGTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAG12330.15619794193693048No Hit
ATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCA12230.15493112975577128No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12200.1545510861014235No Hit
GTGCAGCCACAGTTCGTTTGATTTCTACCTTGGTCCCCTGGCCGAACGTC12050.1526508678296847No Hit
CATCAGTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCCCTTGGACGTTCGG12040.15252418661156877No Hit
ACCAAATACTACGCAGGCTCTGTGAAGGGCCGATTTACCATCTCCAGGGACAACGCCAAGAACTCACTGTATCTG11940.15125737443040957No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11670.14783698154127972No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11450.14504999474272945No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11180.1416296018535996No Hit
ACAGTACCCCTTGGACGTTCGGCCAGGGGACCAAGGTAGAAATCAAACGA10930.13846257140070156No Hit
GTACTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTAC10890.13795584652823786No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10680.13529554094780352No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG10620.13453545363910802No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10310.13060833587751447No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10280.1302282922231667No Hit
GGGAAAGCCCCTGACCTCCTGATCTATGCTACATCCAATTTGCAAAGTGG9880.12516104349852986No Hit
CAGTAATACACGGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAGATA9780.12389423131737066No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9730.12326082522679106No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9700.12288078157244328No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGGAGCCTCTGGATTCACCTTC9660.12237405669997961No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACTTCCAGATGACCCAGTCTCCATCCTCC9620.12186733182751593No Hit
AGTCAGAGCATTAGCACCTATTTAAATTGGTATCGGCAGAGACCAGGGAA9480.12009379477389304No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9470.1199671135557771No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA9280.1175601704115746No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT9250.11718012675722685No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9180.1162933582304154No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC9120.11553327092171986No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9090.11515322726737211No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9080.1150265460492562No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9050.11464650239490842No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9040.1145198211767925No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9020.11426645874056066No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9020.11426645874056066No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8970.11363305264998107No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8950.11337969021374922No Hit
GGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGAGTACTGATGGTGAGAGTGAAAT8930.11312632777751737No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC8820.11173283437824226No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8800.11147947194201041No Hit
GCATTAGCACCTATTTAAATTGGTATCGGCAGAGACCAGGGAAAGCCCCT8710.11033934097896712No Hit
CTCCTGATCTATGCTACATCCAATTTGCAAAGTGGGGTCCCATCAAGGTT8690.11008597854273527No Hit
GTGTATTACTGTGTGAGAATCTGGTGGCGTATGCTTCAGGGAGTTCCCCT8580.10869248514346014No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA8330.10552545469056213No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC8290.10501872981809844No Hit
CTCCTGTGGAGCCTCTGGATTCACCTTCAGTGATTATTATATGGGTTGGATTCGCCAGGCCCCAGGGAAGGGG8260.10463868616375069No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8240.10438532372751884No Hit
GATCAGGAGGTCAGGGGCTTTCCCTGGTCTCTGCCGATACCAATTTAAAT8150.10324519276447555No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8150.10324519276447555No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8140.10311851154635962No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA8120.10286514911012778No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT8060.10210506180143225No Hit
CCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG7970.10096493083838896No Hit
GTATTTGGTAGTACCACTATCACTAATGTGAGAAATCCACTGCAGCCCCTTCCCTGGGGCCTGGCGAATCCAACC7960.10083824962027306No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT7930.10045820596592528No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT7900.10007816231157751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)